Application
Flow Cytometry
Sample
Rhesus monkey Cell (chorioretinal endothelial cell)
Permeabilization
No
Gating Strategy
size and granularity
Specification
chorioretinal endothelial cell
Preparation
Cell harvesting/tissue preparation method: cells were detached from tissue culture plates with Accutase
Sample buffer: 2mM EDTA + 0.5% BSA in PBS
Sample buffer: 2mM EDTA + 0.5% BSA in PBS
Fixation
Paraformaldehyde
Other product details
Incubation time
20 minute(s) · Temperature: 4°C · Diluent: 2mM EDTA + 0.5% BSA in PBS
Dilution
1/100
Secondary antibody
Dilution
1/200
Name
Non-Abcam antibody was used: Vector Labs R-Phycoerythrin Horse Anti-Mouse
Host species: Mouse
Clonality: Monoclonal
Conjugation: Phycoerythrin
Host species: Mouse
Clonality: Monoclonal
Conjugation: Phycoerythrin
Additional data
Abcam response
Thank you for taking part in our AbTrial program and your valuable feedback in Flow Cytometry and Rhesus Monkey. Both species and application have not been tested before.
We encourage all researchers to contact us should they wish to test any of our products in untested species or applications, as these may qualify for our AbTrial program.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Andrea Cabrera
Verified customer
投稿 Sep 22 2017