Anti-Heparan Sulfate Proteoglycan 2/Perlecan 抗体 [A76] (ab26265)
Key features and details
- Mouse monoclonal [A76] to Heparan Sulfate Proteoglycan 2/Perlecan
- Suitable for: IP, WB, ELISA, IHC-Fr, IHC-P
- Reacts with: Sheep, Cow, Human
- Isotype: IgG1
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-Heparan Sulfate Proteoglycan 2/Perlecan antibody [A76]
Heparan Sulfate Proteoglycan 2/Perlecan 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [A76] to Heparan Sulfate Proteoglycan 2/Perlecan -
由来種
Mouse -
特異性
This antibody is highly specific for Heparan Sulfate Proteoglycan 2/Perlecan. There is no evidence for cross-reactivity with other connective tissue proteins (vitronectin, fibronectin, elastin, collagen, laminin) but it does cross-react with human thrombospondin.
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アプリケーション
適用あり: IP, WB, ELISA, IHC-Fr, IHC-Pmore details -
種交差性
交差種: Sheep, Cow, Human -
免疫原
Tissue, cells or virus corresponding to Cow Heparan Sulfate Proteoglycan 2/Perlecan.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7.40
Preservative: 0.097% Sodium azide
Constituents: 0.0268% PBS, 2.9% Sodium chloride -
Concentration information loading...
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精製度
Protein G purified -
特記事項(精製)
Protein-A/G purified. -
ポリ/モノ
モノクローナル -
クローン名
A76 -
ミエローマ
Sp2/0 -
アイソタイプ
IgG1 -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab26265の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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IP |
Use at an assay dependent concentration.
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WB |
Use at an assay dependent concentration.
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ELISA |
Use at an assay dependent concentration.
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IHC-Fr |
Use at an assay dependent concentration.
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IHC-P |
Use at an assay dependent concentration. PubMed: 18568676
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AP |
Use at an assay dependent concentration.
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特記事項 |
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IP
Use at an assay dependent concentration. |
WB
Use at an assay dependent concentration. |
ELISA
Use at an assay dependent concentration. |
IHC-Fr
Use at an assay dependent concentration. |
IHC-P
Use at an assay dependent concentration. PubMed: 18568676 |
AP
Use at an assay dependent concentration. |
ターゲット情報
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機能
Integral component of basement membranes. Component of the glomerular basement membrane (GBM), responsible for the fixed negative electrostatic membrane charge, and which provides a barrier which is both size- and charge-selective. It serves as an attachment substrate for cells. Plays essential roles in vascularization. Critical for normal heart development and for regulating the vascular response to injury. Also required for avascular cartilage development.
Endorepellin in an anti-angiogenic and anti-tumor peptide that inhibits endothelial cell migration, collagen-induced endothelial tube morphogenesis and blood vessel growth in the chorioallantoic membrane. Blocks endothelial cell adhesion to fibronectin and type I collagen. Anti-tumor agent in neovascularization. Interaction with its ligand, integrin alpha2/beta1, is required for the anti-angiogenic properties. Evokes a reduction in phosphorylation of receptor tyrosine kinases via alpha2/beta1 integrin-mediated activation of the tyrosine phosphatase, PTPN6.
The LG3 peptide has anti-angiogenic properties that require binding of calcium ions for full activity. -
組織特異性
Found in the basement membranes. -
関連疾患
Defects in HSPG2 are the cause of Schwartz-Jampel syndrome (SJS1) [MIM:255800]; a rare autosomal recessive disorder characterized by permanent myotonia (prolonged failure of muscle relaxation) and skeletal dysplasia, resulting in reduced stature, kyphoscoliosis, bowing of the diaphyses and irregular epiphyses.
Defects in HSPG2 are the cause of dyssegmental dysplasia Silverman-Handmaker type (DDSH) [MIM:224410]. The dyssegmental dysplasias are rare, autosomal recessive skeletal dysplasias with anisospondyly and micromelia. There are two recognized types: the severe, lethal DDSH and the milder Rolland-Desbuquois form. Individuals with DDSH also have a flat face, micrognathia, cleft palate and reduced joint mobility, and frequently have an encephalocoele. The endochondral growth plate is short, the calcospherites (which are spherical calcium-phosphorus crystals produced by hypertrophic chondrocytes) are unfused, and there is mucoid degeneration of the resting cartilage. -
配列類似性
Contains 4 EGF-like domains.
Contains 22 Ig-like C2-type (immunoglobulin-like) domains.
Contains 11 laminin EGF-like domains.
Contains 3 laminin G-like domains.
Contains 3 laminin IV type A domains.
Contains 4 LDL-receptor class A domains.
Contains 1 SEA domain. -
翻訳後修飾
Proteolytic processing produces the C-terminal angiogenic peptide, endorepellin. This peptide can be further processed to produce the LG3 peptide.
N- and O-glycosylated; contains three heparan sulfate chains. The LG3 peptide contains at least three and up to five potential O-glycosylation sites but no N-glycosylation. -
細胞内局在
Secreted > extracellular space > extracellular matrix > basement membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 444872 Cow
- Entrez Gene: 3339 Human
- Omim: 142461 Human
- SwissProt: P98160 Human
- Unigene: 562227 Human
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別名
- Basement membrane specific heparan sulfate proteoglycan core protein antibody
- Endorepellin (domain V region) antibody
- Heparan sulfate proteoglycan of basement membrane antibody
see all
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (8)
ab26265 は 8 報の論文で使用されています。
- Beyer S et al. Lectin Staining of Microvascular Glycocalyx in Microfluidic Cancer Cell Extravasation Assays. Life (Basel) 11:N/A (2021). PubMed: 33668945
- Salameh S et al. A perfusable vascularized full-thickness skin model for potential topical and systemic applications. Biofabrication 13:N/A (2021). PubMed: 33910175
- Smith SM & Melrose J Type XI collagen-perlecan-HS interactions stabilise the pericellular matrix of annulus fibrosus cells and chondrocytes providing matrix stabilisation and homeostasis. J Mol Histol 50:285-294 (2019). PubMed: 30993430
- Bird IM et al. The skeletal phenotype of achondrogenesis type 1A is caused exclusively by cartilage defects. Development 145:N/A (2018). PubMed: 29180569
- Puperi DS et al. 3-Dimensional spatially organized PEG-based hydrogels for an aortic valve co-culture model. Biomaterials 67:354-64 (2015). PubMed: 26241755
- Rees MD et al. Myeloperoxidase-derived oxidants selectively disrupt the protein core of the heparan sulfate proteoglycan perlecan. Matrix Biol 29:63-73 (2010). WB, ELISA ; Human . PubMed: 19788922
- Smith SM et al. Comparative immunolocalisation of perlecan with collagen II and aggrecan in human foetal, newborn and adult ovine joint tissues demonstrates perlecan as an early developmental chondrogenic marker. Histochem Cell Biol 134:251-63 (2010). IHC-P ; Human, Sheep . PubMed: 20690028
- Melrose J et al. The use of Histochoice for histological examination of articular and growth plate cartilages, intervertebral disc and meniscus. Biotech Histochem 83:47-53 (2008). IHC-P ; Sheep . PubMed: 18568676