Anti-Hemoglobin subunit zeta + Hemoglobin subunit alpha 抗体 [EP3607] (ab109444)

Vielen Dank für Ihren Anruf.

Es tut mir leid, dass Sie Probleme mit diesem Antikörper hatten. Wie am Telefon besprochen habe ich eine kostenlose Ersatzlieferung für Sie in Auftrag gegeben. Sie hat die Referenznummer 123456und sollteMorgen bei Ihnen ankommen.

Ich wünsche Ihnen viel Erfolg für Ihr Projekt. Bitte zögern Sie nicht, sich wieder bei uns zu melden, falls Sie weitere Fragen haben.

Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement with the order number xxx with ab92492. The estimated delivery date is xxx as the item is currently backordered. I hope this is OK.

To check the status of the order please contact our Customer Service team and reference this number.

Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know.

I wish you the best of luck with your research.

Thank you for your reply and for testing the antibodies.

Unfortunately, we do not have another lot in stock for ab109444 - just the same lot. Please let me know if one of the other Anti-Hemoglobin subunit alpha antibodies we carry, ab92492 or ab102758, would be a suitable replacement.

Also, please let me know the species of the protein you tested (human, mouse or rat).
Originally, when you had called, the antibodies gave you high background according to our records, but from the dot blot you sent it looks now more like a weak signal/no staining.
Could you please let me know what samples were used for the original WB and what primary antibody dilution was used then? Also, please confirm if indeed high background was the issue.

We would need this information to update our records as we evaluatethe complaints we receive.

I look forward to hear back from you and resolve this case for you.

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According to our records, ab6721 or ab109444 was proving difficult to use in WBand we were in contact in order to help resolve the issue.

Looking at our correspondence, it appears that we are awaiting more details in order to help us better understand the difficulties experienced.

As we discussed over the phone, trying a no-primary control (with secondary antibody only) would be another way to check if the problem comes from the primary or secondary antibody.

Please let me know if thisproblem hasbeenresolved. If not, please let me know as well, so that we can resolve it in a different way (e.g. replacement, credit or refund).

If the requested information has already been sent, it appears that it did not reach our Scientific Support team and we apologize for this inconvenience. In this case we would like to ask for the information again so that we can reach a resolution.

If the issue has already been settled, please let us know so that we can be assured that the problem has been solved to your satisfaction and update our records.

Iwish you the best of luck with your research and look forward to a reply.

Thank you for contacting us.
I am sorry to hear that one of these antibodies is notworking properly.

As we discussed over the phone, trying a no-primary control (with secondary antibody only) would be another way to check if the problem comes from the primary or secondary antibody.

I look forard to hear back from and assist you in resolving this problem.

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Thank you for the reply.

The recommended dilutions on our datasheets provide a concentration to begin with, however the optimal dilution does have to be determined by the end user. Therefore I strongly recommend that you try various concentrations of the primary antibody, as there is still a good chance that this antibody will work for you, if you can identify the optimal dilution.

Also, I understand that you are worried about high background but could you try and increase the concentration of the protein loaded to 10ug.

If after you have tried the above suggestions you are still not seeing a signal from ab109444, then I would be happy to refund or replace the antibody.

Please let me know if there is anything else I can help you with.

Thank you for sending that protocol information.

If I have read the information correctly, then there are a few protocol variations that may work:

1 - Did the customer only try a 1/5000 primary antibody dilution? If so, I strongly suggest that they try range of dilutions from 1/500-1/2000, to see if they can find the concentration that will work for them.

2 - According to the protocol, they loaded 5ug of protein on the gel. Did they try increasing the amount of protein, to say 20ug?

3 - How long did they incubate the primary antibody for? We recommend overnight at 4C.

4 - Did they try using 5% milk as a blocking reagent instead of BSA?

If the customer has not tried the protocol variations above, then I would strongly suggest that they do. If they have already tried those variations, then I would be happy to process a refund for this antibody, as it is not working as we guarantee.

I look forward to your reply.

I am sorry that the replacement antibody did not prove to succesful. Unfortunately the protocol that you attached did not come through. Could you resend the protocol so that I can look it over and see if there are any suggestions that I can make.

I look forward to your reply.