Anti-Hemoglobin subunit beta 抗体 [EPR20614] - BSA and Azide free (ab240390)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR20614] to Hemoglobin subunit beta - BSA and Azide free
- Suitable for: WB, IP
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
製品の概要
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製品名
Anti-Hemoglobin subunit beta antibody [EPR20614] - BSA and Azide free
Hemoglobin subunit beta 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR20614] to Hemoglobin subunit beta - BSA and Azide free -
由来種
Rabbit -
アプリケーション
適用あり: WB, IPmore details -
種交差性
交差種: Mouse, Rat, Human -
免疫原
This product was produced with the following immunogens:
Recombinant full length protein. This information is proprietary to Abcam and/or its suppliers.
Recombinant full length protein within Human Hemoglobin subunit beta aa 1 to the C-terminus. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: P68871 -
ポジティブ・コントロール
- WB: Rat heart lysate; Mouse heart and lung lysates.
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特記事項
ab240390 is the carrier-free version of ab214049.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
バッファー
pH: 7.2
Constituent: PBS -
キャリア・フリー
はい -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR20614 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Recombinant Protein
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Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab240390の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
Use at an assay dependent concentration. Detects a band of approximately 13 kDa (predicted molecular weight: 15 kDa).
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IP |
Use at an assay dependent concentration.
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特記事項 |
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WB
Use at an assay dependent concentration. Detects a band of approximately 13 kDa (predicted molecular weight: 15 kDa). |
IP
Use at an assay dependent concentration. |
ターゲット情報
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機能
Involved in oxygen transport from the lung to the various peripheral tissues.
LVV-hemorphin-7 potentiates the activity of bradykinin, causing a decrease in blood pressure.
Spinorphin: functions as an endogenous inhibitor of enkephalin-degrading enzymes such as DPP3, and as a selective antagonist of the P2RX3 receptor which is involved in pain signaling, these properties implicate it as a regulator of pain and inflammation. -
組織特異性
Red blood cells. -
関連疾患
Heinz body anemias
Beta-thalassemia
Sickle cell anemia
Beta-thalassemia, dominant, inclusion body type -
配列類似性
Belongs to the globin family. -
翻訳後修飾
Glucose reacts non-enzymatically with the N-terminus of the beta chain to form a stable ketoamine linkage. This takes place slowly and continuously throughout the 120-day life span of the red blood cell. The rate of glycation is increased in patients with diabetes mellitus.
S-nitrosylated; a nitric oxide group is first bound to Fe(2+) and then transferred to Cys-94 to allow capture of O(2).
Acetylated on Lys-60, Lys-83 and Lys-145 upon aspirin exposure. - Information by UniProt
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参照データベース
- Entrez Gene: 3043 Human
- Entrez Gene: 15127 Mouse
- Entrez Gene: 24440 Rat
- Omim: 141900 Human
- SwissProt: P68871 Human
- Unigene: 523443 Human
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別名
- Beta-globin antibody
- HBB antibody
- HBB_HUMAN antibody
see all
画像
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Hemoglobin subunit beta was immunoprecipitated from 0.35 mg of TF-1 (human bone marrow erythroleukemia cell line) lysate with ab214049 at 1/40 dilution. Western blot was performed from the immunoprecipitate using ab214049 at 1/1,000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10,000 dilution.
Lane 1: TF-1 whole cell lysate 10 µg (Input).
Lane 2: ab214049 IP in TF-1 whole cell lysate (+).
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab214049 in TF-1 whole cell lysate (-).
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 3 minutes.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab214049).
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All lanes : Anti-Hemoglobin subunit beta antibody [EPR20614] (ab214049) at 1/1000 dilution
Lane 1 : Rat heart lysate at 20 µg
Lane 2 : Mouse heart lysate at 20 µg
Lane 3 : Mouse lung lysate at 10 µg
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Developed using the ECL technique.
Predicted band size: 15 kDa
Observed band size: 13 kDa why is the actual band size different from the predicted?Blocking/Dilution buffer: 5% NFDM/TBST.
Exposure times: Lane 1-2: 3 minutes; Lane 3: 10 seconds.This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab214049).
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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Datasheet download
Certificate of Compliance
参考文献 (0)
ab240390 は論文での使用が確認できていません。