Application
Western blot
Sample
Human Cell lysate - whole cell (DU145)
Gel Running Conditions
Reduced Denaturing (10)
Loading amount
25 µg
Treatment
20nM siNeg or siNFE2L2 for 24, 48 and 72h
Specification
DU145
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% milk in 1xTBST
Dilution
1/500
Secondary antibody
Dilution
1/10000
Name
Non-Abcam antibody was used: IRDye 800CW Goat anti-Mouse
Host species: Goat
Clonality: Polyclonal
Conjugation: IRDye® 800CW
Host species: Goat
Clonality: Polyclonal
Conjugation: IRDye® 800CW
Detection
Detection method
LI-COR scan
Exposure
3 minute(s) and 0 second(s)
Bands
Specific: Approx. 30 kDa Non-specific: Approx. 33 kDa
Additional data
Additional Notes
Lane 1: siNeg 24h, lane 2: siNFE2L2 24h, lane 3: siNeg 48h, lane 4: siNFE2L2 48h, lane 5: siNeg 72h, lane 6: siNFE2L2 72h
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Jan 17 2019