Application
Western blot
Loading amount
20 µg
Gel Running Conditions
Reduced Denaturing (8%)
Sample
Rat Cell lysate - whole cell (RAT AORTIC SMOOTH MUSCLE CELLS)
Specification
RAT AORTIC SMOOTH MUSCLE CELLS
Blocking step
Milk as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Other product details
Incubation time
18 hour(s) and 0 minute(s) · Temperature: 4°C
Secondary antibody
Name
Non-Abcam antibody was used: anti-mouse
Host species: Mouse
Clonality: Monoclonal
Conjugation: Horse Radish Peroxidase
Host species: Mouse
Clonality: Monoclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL
Bands
Specific: 30-40 kDa
Exposure
15 minute(s) and 0 second(s)
Positive control
Rat spleen
Additional data
Abcam response
Heme Oxygenase can be enriched in the sample by preparing a microsomal fraction. The protocol at the following link has instructions for isolating membranes, including microsomes, using ultracentrifugation.
https://www.abcam.com/index.html?pageconfig=resource&rid=11473
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
MR. P Bingi
Verified customer
投稿 Oct 14 2013