Application
Western blot
Sample
Human Cell lysate - whole cell (kidney)
Loading amount
20 µg
Specification
kidney
Treatment
Cadmium
Gel Running Conditions
Reduced Denaturing (4-12% gradient)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Other product details
Dilution
1/250
Incubation time
18 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: TBS-T
Secondary antibody
Name
Non-Abcam antibody was used: GE CY3
Host species: Goat
Clonality: Polyclonal
Conjugation: Cy3 ®
Host species: Goat
Clonality: Polyclonal
Conjugation: Cy3 ®
Dilution
1/5000
Detection
Detection method
Fluorescence
Exposure
2 minute(s) and 0 second(s)
Bands
Specific: 33 kDa Non-specific: 40 kDa
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Dec 01 2011