Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (MDA-MB-231)
Permeabilization
Yes - 0.2% Triton X-100
Specification
MDA-MB-231
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 25°C
Fixative
Paraformaldehyde
Other product details
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 37°C
Dilution
1/200
Secondary antibody
Dilution
1/500
Name
Non-Abcam antibody was used: Alexa Fluor 594- conjugated
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 594
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 594
Additional data
Additional Notes
Cells were grown on sterile coverslips embedded in a 6-well plate. Cells were fixed with 3.7% paraformaldehyde and permeabilized with 0.2% Triton X-100. After blocking with 2% goat serum in PBS, the protein was detected using anti-HO-1 antibodies followed by reaction with Alexa Fluor 594- conjugated secondary antibodies.
For the green channel, it's protein disulfide isomerase-A1.
I incubated the primary antibody in 37 degree for 1 hour.
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投稿 Jan 13 2021