Application
Western blot
Loading amount
10 µg
Gel Running Conditions
Reduced Denaturing
Sample
Mouse Cell lysate - whole cell (RAW 264.7)
Specification
RAW 264.7
Treatment
Control No treatment, 5mM N-ACETYL CYSTEINE, 50uM buthionine sulphoximine
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Other product details
Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C
Dilution
1/2000
Secondary antibody
Name
Non-Abcam antibody was used: Jackson Immuno Research Goat
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/2000
Detection
Detection method
ECL
Bands
Specific: 30-40 kDa
Exposure
2 second(s)
Negative control
NAC
Positive control
BSO
Additional data
Additional Notes
The first Lane is Biotin Ladder
The Second Lane is Control Cells With NO Treatment
The Third Lane is NAC (anti oxidant)
The Fourth Lane is BSO
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Vamsi Kodali
Verified customer
投稿 Jun 06 2014