Hela-DFO treated (0.5mM, 24h) Nuclear Lysate (ab180880)

製品の概要

  • 製品名
    Hela-DFO treated (0.5mM, 24h) Nuclear Lysate
  • 特記事項
    We recommend aliquoting the extracts into single use fractions and then storing them at -80°C.


    The cells were treated for 24 hours with a 0.5mM solution of Deferoxamine (DFO). DFO is used as a hypoxia-mimetic agent to stabilize Hypoxia Inducible Factor 1 (HIF-1). DFO stabilizes HIF-1 through the inhibition of Prolyl Hydroxylases (PHDs) which target HIF-1 through degradation. The mechanism of DFO inhibition is likely through the chelation of Fe2+ bound to the active site of PHD which is required for enzymatic activity.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped on Dry Ice. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.
  • バッファー
    Constituent: 10% DTT
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • ライゼート 備考
    The cells were treated for 24 hours with a 0.5mM solution of Deferoxamine (DFO). DFO is used as a hypoxia-mimetic agent to stabilize Hypoxia Inducible Factor 1 (HIF-1). DFO stabilizes HIF-1 through the inhibition of Prolyl Hydroxylases (PHDs) which target HIF-1 through degradation. The mechanism of DFO inhibition is likely through the chelation of Fe2+ bound to the active site of PHD which is required for enzymatic activity.
  • 背景
    HeLa cells are human epithelial cells from a fatal cervical carcinoma. The cell line was derived from cervical cancer cells taken from Henrietta Lacks, in 1951. Horizontal gene transfer from human papillomavirus 18 (HPV18) to human cervical cells created the HeLa genome which is different from either parent genome in various ways including its number of chromosomes. HeLa cells have a modal chromosome number of 82, with 4 copies of chromosome 12 and 3 copies of chromosomes 6, 8, and 17. HeLa cells are adherent cells (they stick to surfaces) and maintain contact inhibition in vitro.

画像

  • All lanes : Simply Blue Coomassie Stain 20ml

    Lane 1 : HeLa nuclear extract lysate (ab150036)
    Lane 2 : Hela-DFO treated (0.5mM, 24h) Nuclear Lysate (ab180880)

    Lysates/proteins at 10 µg per lane.
  • All lanes : Anti-HIF-1 alpha antibody [H1alpha67] - ChIP Grade (ab1) at 5 µg/ml

    Lane 1 : HeLa whole cell lysate (ab150035)
    Lane 2 : Hela-DFO treated (0.5mM, 24h) Nuclear Lysate (ab180880)

    Lysates/proteins at 40 µg per lane.

    Secondary
    All lanes : Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/10000 dilution
  • All lanes : Anti-HIF-1 alpha antibody [EP1215Y] (ab51608) at 1/2000 dilution

    Lane 1 : HeLa nuclear extract lysate (ab150036)
    Lane 2 : Hela-DFO treated (0.5mM, 24h) Nuclear Lysate (ab180880)

    Lysates/proteins at 40 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution

参考文献

ab180880 has not yet been referenced specifically in any publications.

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