Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR5342] to HEC1/HEC - BSA and Azide free
- Suitable for: WB, IP
- Reacts with: Mouse, Rat, Human
製品名Anti-HEC1/HEC antibody [EPR5342] - BSA and Azide free
HEC1/HEC 一次抗体 製品一覧
製品の詳細Rabbit monoclonal [EPR5342] to HEC1/HEC - BSA and Azide free
アプリケーション適用あり: WB, IPmore details
適用なし: Flow Cyt,ICC or IHC-P
種交差性交差種: Mouse, Rat, Human
Synthetic peptide within Human HEC1/HEC aa 600-700 (C terminal). The exact sequence is proprietary.
ab247878 is the carrier-free version of ab109496 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Ab247878 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product was previously labelled as HEC1
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Concentration information loading...
Our Abpromise guarantee covers the use of ab247878 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent concentration. Predicted molecular weight: 74 kDa.|
|IP||Use at an assay dependent concentration.|
機能Acts as a component of the essential kinetochore-associated NDC80 complex, which is required for chromosome segregation and spindle checkpoint activity. Required for kinetochore integrity and the organization of stable microtubule binding sites in the outer plate of the kinetochore.
配列類似性Belongs to the NDC80/HEC1 family.
発生段階Expression peaks in mitosis.
翻訳後修飾Phosphorylation begins in S phase of the cell cycle and peaks in mitosis. Phosphorylated by NEK2. May also be phosphorylated by AURKA and AURKB.
細胞内局在Nucleus. Chromosome > centromere > kinetochore. Localizes to kinetochores from late prophase to anaphase. Localizes specifically to the outer plate of the kinetochore.
- Information by UniProt
- from Entrez Gene ; antibody
- HEC antibody
- Highly expressed in cancer antibody
All lanes : Anti-HEC1/HEC antibody [EPR5342] (ab109496) at 1/10000 dilution
Lane 1 : Jurkat lysate
Lane 2 : HeLa lysate
Lane 3 : NCCIT lysate
Lane 4 : Human spleen lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 74 kDa
This data was developed using ab109496, the same antibody clone in a different buffer formulation.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.