Heat Mediated High pH Antigen Retrieving Solution (ab972)

Submit a review Q&A (1)References (1)

製品の概要

  • 製品名

    Heat Mediated High pH Antigen Retrieving Solution
    Antigen retrieval solution ihc 試薬 製品一覧

  • アプリケーション

    適用あり: IHC-Pmore details

  • 特記事項

    This antigen retrieval solution (pH 9.5) incorporates a colored pH indicator. The end-user is assured, by visual inspection, that the solution is at the correct dilution and pH. This product is specially formulated for superior pH stability at high temperatures, and will help prevent the possibility of losing pH sensitive antigens. When heating, the solution should turn gray. If the color of the solution turns yellow or red, it is an indication that the pH of the solution is incorrect and that staining may be hindered.


    This one-step high pH solution (9.5) allows the end-user to simultaneously deparaffinize and to heat-pretreat formalin-fixed paraffin-embedded tissues. Xylenes and alcohols are not needed for deparaffinization, thus saving valuable technician time and reduce costs for reagents and hazardous waste. Alternatively, tissues can be deparaffinized in xylene and hydrated to water, and then placed into the solution in the pressure cooker and/or other heating methods. This product also has blocking reagents to reduced non-specific background staining.

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Store at room temperature.

  • バッファー

    pH: 9.50
    Preservative: Proprietary preservative
    Constituents: Carrier protein, PBS
    Note: Proprietary preservative
  • Concentration information loading...

  • IHC 試薬 備考

    This one-step high pH solution (9.5) allows the end-user to simultaneously deparaffinize and to heat-pretreat formalin-fixed paraffin-embedded tissues. Xylenes and alcohols are not needed for deparaffinization, thus saving valuable technician time and reduce costs for reagents and hazardous waste. Alternatively, tissues can be deparaffinized in xylene and hydrated to water, and then placed into the solution in the pressure cooker and/or other heating methods. This product also has blocking reagents to reduced non-specific background staining.

  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab972 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P Use at an assay dependent concentration.

参考文献

This product has been referenced in:

  • Rosen D  et al. Accelerated thymic maturation and autoreactive T cells in bronchopulmonary dysplasia. Am J Respir Crit Care Med 174:75-83 (2006). IHC-P ; Baboon . Read more (PubMed: 16574933) »
See 1 Publication for this product

ab972 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

レビューと Q&A

Show All レビュー Q&A
レビューを送る 質問を送る

Question

Thank you for your answer. I received additional enquiries from the customer. 1. Can you inform us of the temperature of antigen retrieval solution at 2nd part of the procedure shown on the web? 2. Can you inform us of an appropriate time of heating using Copin jar/autoclave? 3. Please let us know if an expiration date is set for ab972. Again, thank you for your assistance.

Read More
Answer

Thank you for your enquiry. Our QC procedure is to heat the Borg up to 125 degrees for 30 second and then cool down to 90 degrees. This procedure takes place in our pressure cooker. The entire process takes 35 minutes. We have not tried it in an autoclave, but it should work and I would use the above temperature and pressure as a guideline. We would expect the product to be sable for up to one year from the time of purchase if stored correctly.

Read More

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

登録