Anti-HDGF 抗体 [EPR7898] - BSA and Azide free (ab248221)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR7898] to HDGF - BSA and Azide free
- Suitable for: IHC-P, Flow Cyt (Intra), WB, ICC/IF
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-HDGF antibody [EPR7898] - BSA and Azide free
HDGF 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR7898] to HDGF - BSA and Azide free -
由来種
Rabbit -
アプリケーション
適用あり: IHC-P, Flow Cyt (Intra), WB, ICC/IFmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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特記事項
ab248221 is the carrier-free version of ab128921.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
バッファー
pH: 7.2
Constituent: PBS -
キャリア・フリー
はい -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR7898 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab248221の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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IHC-P |
Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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Flow Cyt (Intra) |
Use at an assay dependent concentration.
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WB |
Use at an assay dependent concentration. Detects a band of approximately 40 kDa (predicted molecular weight: 27 kDa).
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ICC/IF |
Use at an assay dependent concentration.
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特記事項 |
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IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
Flow Cyt (Intra)
Use at an assay dependent concentration. |
WB
Use at an assay dependent concentration. Detects a band of approximately 40 kDa (predicted molecular weight: 27 kDa). |
ICC/IF
Use at an assay dependent concentration. |
ターゲット情報
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機能
Heparin-binding protein, with mitogenic activity for fibroblasts. Acts as a transcriptional repressor. -
組織特異性
Ubiquitous. -
配列類似性
Belongs to the HDGF family.
Contains 1 PWWP domain. -
ドメイン
The PWWP domain harbors the heparin-binding sites and is responsible for DNA-binding, while the C-terminal region is essentially unstructured. -
翻訳後修飾
Sumoylated by SUMO1. Sumoylation prevents binding to chromatin. -
細胞内局在
Cytoplasm. Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 3068 Human
- Entrez Gene: 15191 Mouse
- Entrez Gene: 114499 Rat
- Omim: 600339 Human
- SwissProt: P51858 Human
- SwissProt: P51859 Mouse
- SwissProt: Q8VHK7 Rat
- Unigene: 506748 Human
see all -
別名
- FLJ96580 antibody
- HDGF antibody
- HDGF_HUMAN antibody
see all
画像
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All lanes : Anti-HDGF antibody [EPR7898] (ab128921) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : A549 cell lysate
Lane 3 : SKBR-3 cell lysate
Lane 4 : ECV-304 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 27 kDaThis data was developed using ab128921, the same antibody clone in a different buffer formulation.
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This data was developed using ab128921, the same antibody clone in a different buffer formulation.ab128921 staining HDGF in MCF-7 (human breast carcinoma) cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 4% PFA and permeabilized with 0.1% triton X-100. Samples were incubated with primary antibody at a dilution of 1/500. A goat anti rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody at a dilution of 1/1000. DAPI was used as a nuclear counterstain and the negative control was PBS only.
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This data was developed using ab128921, the same antibody clone in a different buffer formulation.Overlay histogram showing HeLa cells stained with ab128921 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab128921, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1?g/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
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This data was developed using ab128921, the same antibody clone in a different buffer formulation.ab128921, at a 1/100 dilution, staining HDGF in paraffin embedded Human hepatocellular carcinoma tissue by Immunohistochemistry. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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Datasheet download
Certificate of Compliance
参考文献 (0)
ab248221 は論文での使用が確認できていません。