Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (hiPSC-SMC (smooth muscle cells))
Permeabilization
Yes - 0.05% Triton-X 100
Specification
hiPSC-SMC (smooth muscle cells)
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10%
Fixative
Formaldehyde
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 0.05% Triton-X 100 + 10% FBS in PBS
Dilution
1/400
Secondary antibody
Name
Non-Abcam antibody was used: AlexaFluor 568 Donkey anti-Rabbit (Thermo Fisher)
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor® 568
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor® 568
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
MRS. Alessandra Granata
Verified customer
投稿 Feb 06 2020