製品の概要
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製品名Anti-HDAC6 antibody
HDAC6 一次抗体 製品一覧 -
製品の詳細Rabbit polyclonal to HDAC6
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由来種Rabbit
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特異性The antibody detects ~134 kDa histone deacetylase 6. It does not cross-react with other HDAC proteins including HDAC1, 2, 3, 4, 5, 7, and 8.
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アプリケーション適用あり: IHC-P, WBmore details
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種交差性交差種: Mouse, Rat, Human
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免疫原
Synthetic peptide (Mouse) - N terminal.
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ポジティブ･コントロール
- WB: Jurkat cell lysate.
製品の特性
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製品の状態Liquid
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保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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バッファーPBS pH7.2 with 50% glycerol, 1% BSA and 0.02% sodium azide
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Concentration information loading... -
精製度Immunogen affinity purified
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ポリ/モノポリクローナル
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アイソタイプIgG
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研究分野
関連製品
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Compatible Secondaries
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Immunizing Peptide (Blocking)
アプリケーション
Our Abpromise guarantee covers the use of ab1440 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IHC-P | Use a concentration of 20 µg/ml. | |
| WB | Use a concentration of 0.5 - 4 µg/ml. Detects a band of approximately 134 kDa (predicted molecular weight: 131 kDa).Can be blocked with HDAC6 peptide (ab41708). Block with 5% milk in TBST and incubate the antibody in blocking buffer. |
ターゲット情報
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機能Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes (By similarity). Plays a central role in microtubule-dependent cell motility via deacetylation of tubulin.
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配列類似性Belongs to the histone deacetylase family. HD type 2 subfamily.
Contains 1 UBP-type zinc finger. -
翻訳後修飾Ubiquitinated. Its polyubiquitination however does not lead to its degradation.
Sumoylated in vitro. -
細胞内局在Nucleus. Cytoplasm. It is mainly cytoplasmic, where it is associated with microtubules.
- Information by UniProt
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参照データベース
- Entrez Gene: 10013 Human
- Entrez Gene: 15185 Mouse
- Entrez Gene: 84581 Rat
- Omim: 300272 Human
- SwissProt: Q9UBN7 Human
- SwissProt: Q9Z2V5 Mouse
- Unigene: 6764 Human
- Unigene: 29854 Mouse
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別名
- CPBHM antibody
- FLJ16239 antibody
- HD 6 antibody
see all
画像
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Western blot - Anti-HDAC6 antibody (ab1440)Anti-HDAC6 antibody (ab1440) + Jurkat cell lysate
Predicted band size: 131 kDa
Observed band size: 131 kDa
参考文献
This product has been referenced in:
- Yano M et al. Association of histone deacetylase expression with histology and prognosis of ovarian cancer. Oncol Lett 15:3524-3531 (2018). Read more (PubMed: 29456726) »
- Li C et al. The immunohistochemical expression and potential prognostic value of HDAC6 and AR in invasive breast cancer. Hum Pathol 75:16-25 (2018). Read more (PubMed: 29180246) »
レビューと Q&A
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"