Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Rat Tissue sections (adult Sprague Dawley rat cervical spinal cord)
Antigen retrieval step
None
Permeabilization
Yes - 0.1% Triton X-100
Specification
adult Sprague Dawley rat cervical spinal cord
Blocking step
normal goat serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Fixative
Paraformaldehyde
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 25°C · Diluent: 3%normalgoat serum, 0.1%tritonX-100, PBS
Dilution
1/1000
Secondary antibody
Name
Non-Abcam antibody was used: Molecular Probes
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Additional data
Additional Notes
Image is:
Rat cervical spinal cord section stained with HDAC4 1:1000 (green) (Abcam Ab12171) and NeuN 1:3000 (red) (EMDMillipore Cat# ABN90) and DAPI in blue.
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投稿 Jul 05 2018