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Epigenetics and Nuclear Signaling Chromatin Modifying Enzymes Acetylation
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Anti-HDAC3 抗体 (ab7030)

  • Datasheet
  • SDS
Reviews (10)Q&A (5)References (100)

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Western blot - Anti-HDAC3 antibody - ChIP Grade (ab7030)
  • Immunocytochemistry - Anti-HDAC3 antibody - ChIP Grade (ab7030)
  • Western blot - Anti-HDAC3 antibody - ChIP Grade (ab7030)
  • Immunocytochemistry - Anti-HDAC3 antibody (ab7030)

Key features and details

  • Rabbit polyclonal to HDAC3
  • Suitable for: ICC, WB
  • Reacts with: Mouse, Rat, Human, Monkey, Chinese hamster
  • Isotype: IgG

リコンビナント抗体で、ロット間での高い再現性を実現

Product image
Anti-HDAC3 antibody [Y415] (ab32369)
  • 異なるロット間での安定した再現性
  • 容易なスケールアップ
  • 評価試験による特異性の確認済み
  • 倫理基準に準拠 - アニマル・フリーの生産

製品の概要

  • 製品名

    Anti-HDAC3 antibody
    HDAC3 一次抗体 製品一覧
  • 製品の詳細

    Rabbit polyclonal to HDAC3
  • 由来種

    Rabbit
  • アプリケーション

    適用あり: ICC, WBmore details
  • 種交差性

    交差種: Mouse, Rat, Human, Monkey, Chinese hamster
  • 免疫原

    Synthetic peptide corresponding to Human HDAC3 aa 411-428 conjugated to Keyhole Limpet Haemocyanin (KLH). The epitope recognized by the antibody is resistant to routine formalin-fixation and paraffin-embedding.
    Sequence:

    C-NEFYDGDHDNDKESDVEI

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • 特記事項

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

製品の特性

  • 製品の状態

    Liquid
  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファー

    pH: 7.40
    Preservative: 0.097% Sodium azide
    Constituent: 0.0268% PBS
  • Concentration information loading...
  • 精製度

    IgG fraction
  • 特記事項(精製)

    Whole antiserum is fractionated and further purified by anion-exchange chromatography to provide the IgG fraction of antiserum that is essentially free of other rabbit serum proteins.
  • ポリ/モノ

    ポリクローナル
  • アイソタイプ

    IgG
  • 研究分野

    • Epigenetics and Nuclear Signaling
    • Chromatin Modifying Enzymes
    • Acetylation
    • Stem Cells
    • Signaling Pathways
    • Wnt
    • HDACs
    • Epigenetics and Nuclear Signaling
    • Chromatin Modifying Enzymes
    • Acetylation
    • HDACs
    • Class I
    • Cancer
    • Oncoproteins/suppressors
    • Tumor suppressors
    • Other
    • Cardiovascular
    • Heart
    • Hypertrophy
    • Other
    • Cancer
    • Cancer Metabolism
    • Metabolic signaling pathway
    • Metabolism of lipids and lipoproteins
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Lipid and lipoprotein metabolism
    • Lipid metabolism
    • Metabolism
    • Types of disease
    • Cancer

関連製品

  • ChIP Related Products

    • Rabbit Anti-Mouse IgG H&L (ab46540)
    • ChIP Kit (ab500)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Recombinant Protein

    • Recombinant human HDAC3 protein (ab101663)

アプリケーション

Our Abpromise guarantee covers the use of ab7030 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC 1/200.
WB Use a concentration of 2000 - 10000 µg/ml. Detects a band of approximately 49 kDa (predicted molecular weight: 49 kDa).

ターゲット情報

  • 機能

    Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Probably participates in the regulation of transcription through its binding to the zinc-finger transcription factor YY1; increases YY1 repression activity. Required to repress transcription of the POU1F1 transcription factor. Acts as a molecular chaperone for shuttling phosphorylated NR2C1 to PML bodies for sumoylation.
  • 組織特異性

    Widely expressed.
  • 配列類似性

    Belongs to the histone deacetylase family. HD type 1 subfamily.
  • 翻訳後修飾

    Sumoylated in vitro.
  • 細胞内局在

    Nucleus.
  • Target information above from: UniProt accession O15379 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 参照データベース

    • Entrez Gene: 8841 Human
    • Entrez Gene: 15183 Mouse
    • Entrez Gene: 84578 Rat
    • Omim: 605166 Human
    • SwissProt: O15379 Human
    • SwissProt: O88895 Mouse
    • SwissProt: Q6P6W3 Rat
    • Unigene: 519632 Human
    • Unigene: 20521 Mouse
    • Unigene: 17284 Rat
    see all
  • 別名

    • HD3 antibody
    • HDAC 3 antibody
    • HDAC3 antibody
    • HDAC3_HUMAN antibody
    • Histone deacetylase 3 antibody
    • RPD3 2 antibody
    • RPD3 antibody
    • RPD3-2 antibody
    • SMAP45 antibody
    see all

画像

  • Western blot - Anti-HDAC3 antibody - ChIP Grade (ab7030)
    Western blot - Anti-HDAC3 antibody - ChIP Grade (ab7030)
    All lanes : Anti-HDAC3 antibody (ab7030) at 1/5000 dilution

    Lane 1 : HEK-293T whole cell lysate.
    Lane 2 : HeLa whole cell lysate.
    Lane 3 : K562 whole cell lysate.
    Lane 4 : COS-7 whole cell lysate.
    Lane 5 : CHO whole cell lysate.
    Lane 6 : NIH-3T3 whole cell lysate.
    Lane 7 : Neuro-2a whole cell lysate.
    Lane 8 : PC-12 whole cell lysate.
    Lane 9 : NRK whole cell lysate.

    Secondary
    All lanes : Goat Anti-Rabbit IgG-Peroxidase and a chemiluminescent substrate.

    Predicted band size: 49 kDa

  • Immunocytochemistry - Anti-HDAC3 antibody - ChIP Grade (ab7030)
    Immunocytochemistry - Anti-HDAC3 antibody - ChIP Grade (ab7030)

    Immunocytochemical immunofluorescence analysis of HeLa cells labelling HDAC3 with ab7030. Cells were fixed and permeabilized with cold methanol followed by a cold methanol and acetone solution. Fixed cells were stained with ab7030 at a 1/200 concentration. The secondary used was a Goat Anti-Rabbit IgG, Cy3 conjugate.

  • Western blot - Anti-HDAC3 antibody - ChIP Grade (ab7030)
    Western blot - Anti-HDAC3 antibody - ChIP Grade (ab7030)This image is courtesy of an anonymous Abreview
    All lanes : Anti-HDAC3 antibody (ab7030) at 1/10000 dilution

    Lane 1 : Whole cell lysate from human HEK293 cell line
    Lane 2 : Whole cell lysate from human HEK293 cell line treated with HDAC3 gene silencing shRNA

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : HRP-conjugated goat anti-rabbit Ig at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 49 kDa


    Exposure time: 10 seconds

    See Abreview

  • Immunocytochemistry - Anti-HDAC3 antibody (ab7030)
    Immunocytochemistry - Anti-HDAC3 antibody (ab7030)This image is courtesy of Michael Mancini, Baylor College of Medicine
    Cells were fixed with 4% formaldehyde in PEM buffer. The coverslip was incubated in blocking buffer of 5% powdered milk in TBS-T plus 0.02% sodium azide for 1 hour at room temperature. Blocking buffer was removed and primary antibody was added at a dilution of 1/5000 and incubated overnight at 4 degrees celsius. The coverslips were then washed 4-5 times with blocking buffer for 5 minutes. Secondary antibody, goat anti-rabbit Alexa 594, was added at a dilution of 1/1000 and incubated at room temperature for one hour. From this point on coverslips were covered with foil to protect them from light. They were washed 5 times with TBS-T and then one time with PEM, for 5 minutes each wash. The coverslips were fixed 10-30 minutes in 4% formaldehyde in PEM buffer, then washed 3 times with PEM buffer for 5 minutes. 0.1M ammonium chloride in PEM buffer was added for 10 minutes to quench auto-florescence, and then slips were washed 2 times for 5 minutes in PEM followed by 3 washes for 5 minutes in

プロトコール

  • ChIP protocols
  • Immunoprecipitation protocols
  • Immunocytochemistry & immunofluorescence protocols
  • Western blot protocols

Click here to view the general protocols

データシートおよび資料

    • Datasheet
    • SDS
  • 参考文献 (100)

    ab7030 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab7030 は 100 報の論文で使用されています。

    • Cheng S  et al. Romidepsin (FK228) in a Mouse Model of Lipopolysaccharide-Induced Acute Kidney Injury is Associated with Down-Regulation of the CYP2E1 Gene. Med Sci Monit 26:e918528 (2020). PubMed: 31954012
    • Ferrante F  et al. HDAC3 functions as a positive regulator in Notch signal transduction. Nucleic Acids Res 48:3496-3512 (2020). PubMed: 32107550
    • Elmansi AM  et al. Age-related increase of kynurenine enhances miR29b-1-5p to decrease both CXCL12 signaling and the epigenetic enzyme Hdac3 in bone marrow stromal cells. Bone Rep 12:100270 (2020). PubMed: 32395570
    • Sun J  et al. N-terminal truncated carboxypeptidase E represses E-cadherin expression in lung cancer by stabilizing the Snail-HDAC complex. Am J Cancer Res 10:925-938 (2020). PubMed: 32266100
    • Aboukhatwa SM  et al. Structurally Diverse Histone Deacetylase Photoreactive Probes: Design, Synthesis, and Photolabeling Studies in Live Cells and Tissue. ChemMedChem 14:1096-1107 (2019). PubMed: 30921497
    View all Publications for this product

    レビューと Q&A

    Show All レビュー Q&A
    レビューを送る 質問を送る

    1-10 of 15 Abreviews or Q&A

    Question

    Inquiry: Dear Scientific Support group, We have been using ab16047 Ab to detect HDAC3 in WB for many years, results are always consistent. I recently performed a ChIP assay using this Ab, but it failed. I know that this Ab is not recommended for ChIP. I found another Ab #ab7030, which is ChIP grade. I also noticed that both of these HDAC3 antibodies are raised against C-terminus of human HDAC3. I was wondering what makes these two Abs so different in terms of ChIP assays, since they both detect the similar region. I was wondering whether you have documented this difference? In addition, which HDAC3 Ab do you recommend for ChIP assay based on your experience? Thanks, Yue

    Read More

    Abcam community

    Verified customer

    Asked on Nov 15 2012

    Answer

    Thank you for contacting us.

    While the specific immunogen for both ab16047 and ab7030 are similar, some antibodies work better in certain applications due to the nature in which they are derived, stored, and their specific affinity for the antigen. Specifically, our "ChIP Grade" antibodies such as the ab7030 that you mentioned have been tested and validated to work specifically in ChIP.

    I recommend the HDAC3 ChIP Grade antibody that you referenced (ab7030: https://www.abcam.com/hdac3-antibody-chip-grade-ab7030.html) as it has 10 Abreviews with an average rating of 4/5 stars (2 Abreviews for ChIP) and 20 specific references.

    For your convencience, I've included links to the 2 ChIP Abreviews for ab7030 below:
    https://www.abcam.com/HDAC3-antibody-ab7030.html&tab=abreviews&intabreviewid=25057
    https://www.abcam.com/HDAC3-antibody-ab7030.html&tab=abreviews&intabreviewid=18386


    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Use our products? Submit an Abreview. Earn rewards!
    https://www.abcam.com/abreviews

    Read More

    Abcam Scientific Support

    Answered on Nov 15 2012

    Western blot abreview for Anti-HDAC3 antibody - ChIP Grade

    Below Average
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Monkey Cell lysate - whole cell (Brain)
    Gel Running Conditions
    Reduced Denaturing (4-20%)
    Loading amount
    15 µg
    Specification
    Brain
    Blocking step
    Milk as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
    Read More

    Abcam user community

    Verified customer

    投稿 May 29 2015

    ChIP abreview for Anti-HDAC3 antibody - ChIP Grade

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    ChIP
    Detection step
    Real-time PCR
    Sample
    Mouse Cell lysate - whole cell (Embryonic Stem Cell)
    Specification
    Embryonic Stem Cell
    Negative control
    Immuno-precipitation with non-specific IgG antibody.
    Type
    Cross-linking (X-ChIP)
    Duration of cross-linking step: 60 minute(s) and 0 second(s)
    Specification of the cross-linking agent: formaldehyde
    Positive control
    WT embryonic stem cells treated with all-trans retinoic acid (1uM)
    Read More

    Abcam user community

    Verified customer

    投稿 Feb 04 2015

    Question

    I just purchased anti-HDAC3 antibody (ab7030) from abcam. The western blot with NIH3T3 cell lysate showes two band, one is a little bigger than 56KDa and the other is a little smalled than 56KDa. I suppose the samller band is what indicate on the datasheet as 49KDa HDAC3 band. Please see the attached picture.

    1.Could you please let meknow why there is a non specific band? any other people showed the same result?

    2. I am palnning to use this antibody for ChIP experiment, since this is ChIP grade antibody. Do you think it is okay for ChIP experient since there is non specific band?

    Read More

    Abcam community

    Verified customer

    Asked on Jun 26 2012

    Answer

    Thank you for contacting us.

    The mouse HDAC3 has 2 isoforms: 27 and 48 kDa (http://www.uniprot.org/uniprot/O88895).
    Also, 2 customers had submitted Abreviews for mouse and WB, one of them for 3T3 cells where they also obtained 2 bands (https://www.abcam.com/HDAC3-antibody-ab7030.html&tab=abreviews&intabreviewid=22723).
    I am not sure how their blot compares to yours since the MW markers are not indicated, but please check the link.
    The other review shows one band at 49 kDa for amouse brain sample:
    https://www.abcam.com/HDAC3-antibody-ab7030.html&tab=abreviews&intabreviewid=20928


    Thus, the bands you see could be specific to thesample you are using.It might be worthwhile including a positive human or mouse positive control (e.g. Hek293 cells). Please keep in mind that the human protein has a different set of 2 isoforms: 48.8 and 49.1 kDa (http://www.uniprot.org/uniprot/O15379).


    As for using the antibody in ChIP:
    The antibody has been tested in this application and would be guaranteed. If the 2 bands you are seeing are specific to the NIH3T3 cells, then it would be no problem using the antibody in ChIP.
    Please see also the Abreviews submitted by other customers: https://www.abcam.com/HDAC3-antibody-ChIP-Grade-ab7030-reviews.html


    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Use our products? Submit an Abreview. Earn rewards!
    https://www.abcam.com/abreviews

    Read More

    Abcam Scientific Support

    Answered on Jun 26 2012

    ChIP abreview for Anti-HDAC3 antibody - ChIP Grade

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    ChIP
    Sample
    Human Cell lysate - whole cell (lymphoblastoid cell line)
    Specification
    lymphoblastoid cell line
    Type
    Cross-linking (X-ChIP)
    Duration of cross-linking step: 10 minute(s) and 0 second(s)
    Specification of the cross-linking agent: Formaldehyde
    Detection step
    Real-time PCR
    Positive control
    NFKBIA promoter
    Negative control
    GAPDH coding sequence
    Read More

    Abcam user community

    Verified customer

    投稿 Jul 15 2011

    Western blot abreview for Anti-HDAC3 antibody - ChIP Grade

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Cell lysate - whole cell (3T3)
    Loading amount
    500000 cells
    Specification
    3T3
    Gel Running Conditions
    Reduced Denaturing
    Blocking step
    Milk as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
    Read More

    Abcam user community

    Verified customer

    投稿 Jan 19 2011

    Western blot abreview for Anti-HDAC3 antibody - ChIP Grade

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Tissue lysate - whole (Mouse brain)
    Loading amount
    20 µg
    Specification
    Mouse brain
    Gel Running Conditions
    Reduced Denaturing (4-12%)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More

    Abcam user community

    Verified customer

    投稿 Oct 04 2010

    Western blot abreview for Anti-HDAC3 antibody - ChIP Grade

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (lymphoblastoid cell line)
    Loading amount
    20 µg
    Specification
    lymphoblastoid cell line
    Gel Running Conditions
    Non-reduced Denaturing
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More

    Abcam user community

    Verified customer

    投稿 Jun 02 2010

    ChIP abreview for Anti-HDAC3 antibody - ChIP Grade

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    ChIP
    Sample
    Human Cell lysate - nuclear (Jurkat Cells)
    Specification
    Jurkat Cells
    Type
    Cross-linking (X-ChIP)
    Duration of cross-linking step: 10 minute(s) and 0 second(s)
    Specification of the cross-linking agent: 1% formaldehyde
    Detection step
    Real-time PCR
    Positive control
    Total Histone 3 antibody from Upstate
    Negative control
    IgG
    Read More

    Abcam user community

    Verified customer

    投稿 May 19 2010

    Western blot abreview for Anti-HDAC3 antibody - ChIP Grade

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (HEK293)
    Loading amount
    20 µg
    Specification
    HEK293
    Treatment
    HDAC3 - shRNA 48 hours
    Gel Running Conditions
    Reduced Denaturing (10%)
    Blocking step
    Milk as blocking agent for 18 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
    Read More

    Abcam user community

    Verified customer

    投稿 Oct 10 2008

    1-10 of 15 Abreviews or Q&A

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