Anti-HDAC3 抗体 (ab7030)
Key features and details
- Rabbit polyclonal to HDAC3
- Suitable for: ICC, ICC/IF, IHC-P, ChIP, WB, Dot blot, IP
- Reacts with: Mouse, Rat, Human, Monkey, African green monkey, Chinese hamster
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-HDAC3 antibody
HDAC3 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to HDAC3 -
由来種
Rabbit -
アプリケーション
適用あり: ICC, ICC/IF, IHC-P, ChIP, WB, Dot blot, IPmore details -
種交差性
交差種: Mouse, Rat, Human, Monkey, African green monkey, Chinese hamster -
免疫原
Synthetic peptide corresponding to Human HDAC3 aa 411-428 conjugated to Keyhole Limpet Haemocyanin (KLH). The epitope recognized by the antibody is resistant to routine formalin-fixation and paraffin-embedding.
Sequence:C-NEFYDGDHDNDKESDVEI
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.097% Sodium azide
Constituent: 0.0268% PBS -
Concentration information loading... -
精製度
IgG fraction -
特記事項（精製）
Whole antiserum is fractionated and further purified by anion-exchange chromatography to provide the IgG fraction of antiserum that is essentially free of other rabbit serum proteins. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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ChIP Related Products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab7030の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ICC |
1/200.
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| ICC/IF |
Use at an assay dependent concentration.
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| IHC-P | (1) |
Use at an assay dependent concentration.
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| ChIP | (3) |
Use at an assay dependent concentration. PubMed: 18830415
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| WB | (6) |
Use a concentration of 2000 - 10000 µg/ml. Detects a band of approximately 49 kDa (predicted molecular weight: 49 kDa).
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| Dot blot |
Use at an assay dependent concentration.
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| IP |
Use at an assay dependent concentration.
|
| 特記事項 |
|---|
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ICC
1/200. |
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ICC/IF
Use at an assay dependent concentration. |
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IHC-P
Use at an assay dependent concentration. |
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ChIP
Use at an assay dependent concentration. PubMed: 18830415 |
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WB
Use a concentration of 2000 - 10000 µg/ml. Detects a band of approximately 49 kDa (predicted molecular weight: 49 kDa). |
|
Dot blot
Use at an assay dependent concentration. |
|
IP
Use at an assay dependent concentration. |
ターゲット情報
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機能
Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Probably participates in the regulation of transcription through its binding to the zinc-finger transcription factor YY1; increases YY1 repression activity. Required to repress transcription of the POU1F1 transcription factor. Acts as a molecular chaperone for shuttling phosphorylated NR2C1 to PML bodies for sumoylation. -
組織特異性
Widely expressed. -
配列類似性
Belongs to the histone deacetylase family. HD type 1 subfamily. -
翻訳後修飾
Sumoylated in vitro. -
細胞内局在
Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 8841 Human
- Entrez Gene: 15183 Mouse
- Entrez Gene: 84578 Rat
- Omim: 605166 Human
- SwissProt: O15379 Human
- SwissProt: O88895 Mouse
- SwissProt: Q6P6W3 Rat
- Unigene: 519632 Human
see all -
別名
- HD3 antibody
- HDAC 3 antibody
- HDAC3 antibody
see all
画像
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Western blot - Anti-HDAC3 antibody - ChIP Grade (ab7030)All lanes : Anti-HDAC3 antibody (ab7030) at 1/5000 dilution
Lane 1 : HEK-293T whole cell lysate.
Lane 2 : HeLa whole cell lysate.
Lane 3 : K562 whole cell lysate.
Lane 4 : COS-7 whole cell lysate.
Lane 5 : CHO whole cell lysate.
Lane 6 : NIH-3T3 whole cell lysate.
Lane 7 : Neuro-2a whole cell lysate.
Lane 8 : PC-12 whole cell lysate.
Lane 9 : NRK whole cell lysate.
Secondary
All lanes : Goat Anti-Rabbit IgG-Peroxidase and a chemiluminescent substrate.
Predicted band size: 49 kDa -
Immunocytochemistry - Anti-HDAC3 antibody - ChIP Grade (ab7030)Immunocytochemical immunofluorescence analysis of HeLa cells labelling HDAC3 with ab7030. Cells were fixed and permeabilized with cold methanol followed by a cold methanol and acetone solution. Fixed cells were stained with ab7030 at a 1/200 concentration. The secondary used was a Goat Anti-Rabbit IgG, Cy3 conjugate.
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Western blot - Anti-HDAC3 antibody - ChIP Grade (ab7030)This image is courtesy of an anonymous AbreviewAll lanes : Anti-HDAC3 antibody (ab7030) at 1/10000 dilution
Lane 1 : Whole cell lysate from human HEK293 cell line
Lane 2 : Whole cell lysate from human HEK293 cell line treated with HDAC3 gene silencing shRNA
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : HRP-conjugated goat anti-rabbit Ig at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 49 kDa
Exposure time: 10 seconds
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Immunocytochemistry - Anti-HDAC3 antibody (ab7030)This image is courtesy of Michael Mancini, Baylor College of MedicineCells were fixed with 4% formaldehyde in PEM buffer. The coverslip was incubated in blocking buffer of 5% powdered milk in TBS-T plus 0.02% sodium azide for 1 hour at room temperature. Blocking buffer was removed and primary antibody was added at a dilution of 1/5000 and incubated overnight at 4 degrees celsius. The coverslips were then washed 4-5 times with blocking buffer for 5 minutes. Secondary antibody, goat anti-rabbit Alexa 594, was added at a dilution of 1/1000 and incubated at room temperature for one hour. From this point on coverslips were covered with foil to protect them from light. They were washed 5 times with TBS-T and then one time with PEM, for 5 minutes each wash. The coverslips were fixed 10-30 minutes in 4% formaldehyde in PEM buffer, then washed 3 times with PEM buffer for 5 minutes. 0.1M ammonium chloride in PEM buffer was added for 10 minutes to quench auto-florescence, and then slips were washed 2 times for 5 minutes in PEM followed by 3 washes for 5 minutes in
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (113)
ab7030 は 113 報の論文で使用されています。
- Garg M et al. Cardiolipin-mediated PPAR? S112 phosphorylation impairs IL-10 production and inflammation resolution during bacterial pneumonia. Cell Rep 34:108736 (2021). PubMed: 33567272
- Yin H et al. HDAC3 controls male fertility through enzyme-independent transcriptional regulation at the meiotic exit of spermatogenesis. Nucleic Acids Res 49:5106-5123 (2021). PubMed: 33939832
- Liao Y et al. HDAC3 inhibition ameliorates ischemia/reperfusion-induced brain injury by regulating the microglial cGAS-STING pathway. Theranostics 10:9644-9662 (2020). PubMed: 32863951
- Kotwal A & Amere Subbarao S Hsp90 regulates HDAC3-dependent gene transcription while HDAC3 regulates the functions of Hsp90. Cell Signal 76:109801 (2020). PubMed: 33017618
- Sun J et al. N-terminal truncated carboxypeptidase E represses E-cadherin expression in lung cancer by stabilizing the Snail-HDAC complex. Am J Cancer Res 10:925-938 (2020). PubMed: 32266100