Application
Western blot
Sample
Human Cell lysate - whole cell (human glioblastoma cell line)
Loading amount
120 µg
Specification
human glioblastoma cell line
Gel Running Conditions
Reduced Denaturing (10%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Other product details
Dilution
1/250
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: MMP/TBS-T
Secondary antibody
Name
Non-Abcam antibody was used: A-3682 anti-mouse
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/10000
Detection
Detection method
ECL+
Exposure
5 minute(s) and 0 second(s)
Bands
Specific: 55 kDa Non-specific: 45 kDa
Additional data
Additional Notes
we observed a band at aprox. 55kDA and a slight band at 45kDa
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 May 05 2011