Anti-HDAC2 抗体 [HDAC2-62] (ab12169)
![Immunoprecipitation - Anti-HDAC2 antibody [HDAC2-62] (ab12169)](https://www.abcam.co.jp/ps/products/12/ab12169/Images/ab12169-213428-anti-hdac2-antibody-hdac2-62-chip-grade-immunoprecipitation.jpg "Immunoprecipitation - Anti-HDAC2 antibody [HDAC2-62] (ab12169)")
Key features and details
- Mouse monoclonal [HDAC2-62] to HDAC2
- Suitable for: IP, WB
- Reacts with: Mouse, Human
- Isotype: IgG2b
製品の概要
-
製品名
Anti-HDAC2 antibody [HDAC2-62]
HDAC2 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [HDAC2-62] to HDAC2 -
由来種
Mouse -
アプリケーション
適用あり: IP, WBmore details -
種交差性
交差種: Mouse, Human -
免疫原
Synthetic peptide:
SGEKTDTKGTKSEQLSNP
with N-terminally added Cysteine conjugated to KLH, corresponding to amino acids 471-488 of Human HDAC2. -
ポジティブ･コントロール
- Whole cell extracts from NIH-3T3 cells.
-
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.097% Sodium azide
Constituent: 0.0268% PBS -
Concentration information loading... -
精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
HDAC2-62 -
アイソタイプ
IgG2b -
研究分野
関連製品
-
ChIP Related Products
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
-
Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab12169の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IP |
Use at an assay dependent concentration.
|
|
| WB | (3) |
Use a concentration of 0.25 - 0.5 µg/ml. Detects a band of approximately 55 kDa.
|
| 特記事項 |
|---|
|
IP
Use at an assay dependent concentration. |
|
WB
Use a concentration of 0.25 - 0.5 µg/ml. Detects a band of approximately 55 kDa. |
ターゲット情報
-
機能
Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes.
Forms transcriptional repressor complexes by associating with MAD, SIN3, YY1 and N-COR. Interacts in the late S-phase of DNA-replication with DNMT1 in the other transcriptional repressor complex composed of DNMT1, DMAP1, PCNA, CAF1. Deacetylates TSHZ3 and regulates its transcriptional repressor activity. -
組織特異性
Widely expressed; lower levels in brain and lung. -
配列類似性
Belongs to the histone deacetylase family. HD type 1 subfamily. -
翻訳後修飾
S-nitrosylated by GAPDH. In neurons, S-Nitrosylation at Cys-262 and Cys-274 does not affect the enzyme activity but abolishes chromatin-binding, leading to increases acetylation of histones and activate genes that are associated with neuronal development. In embryonic cortical neurons, S-Nitrosylation regulates dendritic growth and branching. -
細胞内局在
Nucleus. - Information by UniProt
-
参照データベース
- Entrez Gene: 3066 Human
- Entrez Gene: 15182 Mouse
- Omim: 605164 Human
- SwissProt: Q92769 Human
- SwissProt: P70288 Mouse
- Unigene: 3352 Human
- Unigene: 19806 Mouse
-
別名
- D10Wsu179e antibody
- HD 2 antibody
- HD2 antibody
see all
画像
-
Immunoprecipitation - Anti-HDAC2 antibody [HDAC2-62] (ab12169)
Western Blot using Rabbit anti-HDAC2
Lane 1 : Anti-HDAC2 antibody [HDAC2-62] (ab12169) at 5 µg
Lane 2 : Anti-HDAC2 antibody [HDAC2-62] (ab12169) at 2.5 µg
Lane 3 : Negative Control at 5 µg
All lanes : HeLa whole cell extract -
![Western blot - Anti-HDAC2 antibody [HDAC2-62] (ab12169)](https://www.abcam.co.jp/ps/products/12/ab12169/Images/ab12169-213427-anti-hdac2-antibody-hdac2-62-chip-grade-western-blot.jpg)
Western blot - Anti-HDAC2 antibody [HDAC2-62] (ab12169)All lanes : Anti-HDAC2 antibody [HDAC2-62] (ab12169) at 0.5 µg/ml
Lane 1 : Hek293T cell Lysate
Lane 2 : HeLa cell Lysate
Lane 3 : Jurkat cell Lysate
Lane 4 : K562 cell Lysate
Lane 5 : Neuro-2a cell Lysate
Lane 6 : NIH-3T3 cell Lysate
Secondary
All lanes : Goat Anti-Mouse IgG-Peroxidase -
![Western blot - Anti-HDAC2 antibody [HDAC2-62] (ab12169)](https://www.abcam.co.jp/ps/products/12/ab12169/Images/ab12169-19685-anti-hdac2-antibody-hdac2-62-chip-grade-western-blot.jpg)
Western blot - Anti-HDAC2 antibody [HDAC2-62] (ab12169)This image is courtesy of an anonymous abreview.Anti-HDAC2 antibody [HDAC2-62] (ab12169) at 1/250 dilution + Human glioblastoma cell line at 120 µg
Secondary
HRP-conjugated Goat anti-Mouse at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Observed band size: 55 kDa why is the actual band size different from the predicted?
Additional bands at: 45 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 5 minutes
プロトコール
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (70)
ab12169 は 70 報の論文で使用されています。
- Yao W et al. Activation of BDNF by transcription factor Nrf2 contributes to antidepressant-like actions in rodents. Transl Psychiatry 11:140 (2021). PubMed: 33627628
- Liu Y et al. Long noncoding RNA LINC00518 induces radioresistance by regulating glycolysis through an miR-33a-3p/HIF-1a negative feedback loop in melanoma. Cell Death Dis 12:245 (2021). PubMed: 33664256
- Ecker J et al. Reduced chromatin binding of MYC is a key effect of HDAC inhibition in MYC amplified medulloblastoma. Neuro Oncol 23:226-239 (2021). PubMed: 32822486
- Beaver M et al. Chromatin and transcriptomic profiling uncover dysregulation of the Tip60 HAT/HDAC2 epigenomic landscape in the neurodegenerative brain. Epigenetics N/A:1-22 (2021). PubMed: 34369292
- Jung H & Seo SB Histone lysine demethylase 3B (KDM3B) regulates the propagation of autophagy via transcriptional activation of autophagy-related genes. PLoS One 15:e0236403 (2020). PubMed: 32716961