Anti-HDAC1 抗体 - ChIP Grade (ab1767)

Submit a review Q&A (3)References (12)

製品の概要

  • 製品名

    Anti-HDAC1 antibody - ChIP Grade
    HDAC1 一次抗体 製品一覧

  • 製品の詳細

    Rabbit polyclonal to HDAC1 - ChIP Grade

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: ICC/IF, IHC - Wholemount, ChIP, IP, WBmore details

  • 種交差性

    交差種: Drosophila melanogaster

  • 免疫原

    Synthetic peptide:

    C-SGSGTGSGAGAKGAKENNI

    , corresponding to amino acids 503-521 of Fruit fly (Drosophila melanogaster) HDAC1

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • 特記事項

    The SIN3/RPD3 deacetylase complex is essential for G(2) phase cell cycle progression and regulation of SMRTER corepressor levels.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab1767 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use at an assay dependent concentration.
IHC - Wholemount Use at an assay dependent concentration. PubMed: 22195015
ChIP Use at an assay dependent concentration.
IP Use at an assay dependent concentration.
WB 1/400. Predicted molecular weight: 65 kDa.

ターゲット情報

  • 機能

    Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Deacetylates SP proteins, SP1 and SP3, and regulates their function. Component of the BRG1-RB1-HDAC1 complex, which negatively regulates the CREST-mediated transcription in resting neurons. Upon calcium stimulation, HDAC1 is released from the complex and CREBBP is recruited, which facilitates transcriptional activation. Deacetylates TSHZ3 and regulates its transcriptional repressor activity. Deacetylates 'Lys-310' in RELA and thereby inhibits the transcriptional activity of NF-kappa-B.

  • 組織特異性

    Ubiquitous, with higher levels in heart, pancreas and testis, and lower levels in kidney and brain.

  • 配列類似性

    Belongs to the histone deacetylase family. HD type 1 subfamily.

  • 翻訳後修飾

    Sumoylated on Lys-444 and Lys-476; which promotes enzymatic activity. Desumoylated by SENP1.
    Phosphorylation on Ser-421 and Ser-423 promotes enzymatic activity and interactions with NuRD and SIN3 complexes.
    Ubiquitinated by CHFR, leading to its degradation by the proteasome.

  • 細胞内局在

    Nucleus.
  • Information by UniProt

  • 参照データベース

    • 別名

      • DKFZp686H12203 antibody
      • GON 10 antibody
      • HD1 antibody
      • HDAC 1 antibody
      • HDAC1 antibody
      • HDAC1_HUMAN antibody
      • Histone deacetylase 1 antibody
      • Reduced potassium dependency yeast homolog like 1 antibody
      • RPD3 antibody
      • RPD3L1 antibody
      see all

    画像

    • IHC - Wholemount - Anti-HDAC1 antibody - ChIP Grade (ab1767)
      IHC - Wholemount - Anti-HDAC1 antibody - ChIP Grade (ab1767)Image from Fitzsimons HL et al., PLoS One. 2011;6(12):e29171. Epub 2011 Dec 15. Fig 1.; doi:10.1371/journal.pone.0029171 December 15, 2011, PLoS ONE 6(12): e29171.
      Immunohistochemical analysis of whole mount Drosophila melanogaster brain, staining HDAC1 with ab1767, at 1/1000 dilution.

    参考文献

    This product has been referenced in:

    • Panikker P  et al. Restoring Tip60 HAT/HDAC2 Balance in the Neurodegenerative Brain Relieves Epigenetic Transcriptional Repression and Reinstates Cognition. J Neurosci 38:4569-4583 (2018). Read more (PubMed: 29654189) »
    • Cabezas-Cruz A  et al. Anaplasma phagocytophilum increases the levels of histone modifying enzymes to inhibit cell apoptosis and facilitate pathogen infection in the tick vector Ixodes scapularis. Epigenetics 11:303-19 (2016). Read more (PubMed: 27019326) »
    See all 12 Publications for this product

    ab1767 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    レビューと Q&A

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    1-3 of 3 Abreviews or Q&A

    Question

    This antibody says that it works in ChIP, but there is no concentration listed. How can I use this and compare with rabbit IgG of the same concentration?

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    Answer

    Thank you for your enquiry. This antibody is sold as whole antiserum. The IgG concentration has not been determined. However, I would expect it to be within a range of 10-16mgml-1 given that it is rabbit whole serum. We have not received reviews of this product. Please can you detail where you have seen details of the concentration of this antibody? This antibody has been applied by chromatin IP in the following publication; Breiling A et al. General transcription factors bind promoters repressed by Polycomb group proteins. Nature 412:651-5 (2001). PubMed: 11493924 I hope this information helps, please do not hesitate to contact us if you need any more advice or information.

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    Question

    I want to do ChIP with the HDAC1 antibody. I would like to know if western results show non specific bands being recognised. Is there a reference for this antibody, has it been used in any published papers? Could you send me some of your test data for western and ChIP with this antibody? Dilutions at which it has worked for you? conditions for western and ChIP? Could you send me a small aliquot to test. I have brought histone antibodies from you before. One of them works great, the other doesnt. Anyway, any information would be of great help.Thanks

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    Answer

    Thank you for your enquiry. All the information that we have regarding ab1767 is located on the online datasheet. Unfortunately, we are not aware of any publications that feature the use of this antibody. To our knowledge, customized protocols are not required for this antibody. For Western blotting, I would suggest starting at a dilution of 1:100. We do not routinely offer free or trial sized samples for testing purposes. Our policy at Abcam is that if an antibody does not work as specified on the datasheet, we will offer a replacement or reimbursement.

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    Question

    I ordered the anti-HDAC1 antibody (ab1767) that is supposed to be reactive in Drosophila. I performed western blotting on whole Drosophila heads and, at three different dillutions, and obtained multiple strong bands. I would like to know what is the size that has been determined to be HDAC1 (Rpd3) and what are the conditions used for performing such a western blot. Thank you.

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    Answer

    The expected band size is 65 kDa. I would suggest that perhaps you should try using a dilution at 1:100.

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