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  1. Link

    hdac1-antibody-ab7028.pdf

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Epigenetics and Nuclear Signaling Chromatin Modifying Enzymes Acetylation
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Anti-HDAC1 抗体 (ab7028)

  • Datasheet
  • SDS
Reviews (24)Q&A (8)References (213)

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Western blot - Anti-HDAC1 antibody - ChIP Grade (ab7028)
  • Immunocytochemistry - Anti-HDAC1 antibody (ab7028)
  • Immunoprecipitation - Anti-HDAC1 antibody - ChIP Grade (ab7028)
  • Immunocytochemistry - Anti-HDAC1 antibody (ab7028)
  • Western blot - Anti-HDAC1 antibody - ChIP Grade (ab7028)
  • Immunoprecipitation - Anti-HDAC1 antibody - ChIP Grade (ab7028)
  • Western blot - Anti-HDAC1 antibody - ChIP Grade (ab7028)

Key features and details

  • Rabbit polyclonal to HDAC1
  • Suitable for: ICC, WB, IP
  • Reacts with: Mouse, Rat, Human, Chinese hamster
  • Isotype: IgG

リコンビナント抗体で、ロット間での高い再現性を実現

Product image
Anti-HDAC1 antibody [RM1004] (ab281585)
  • 異なるロット間での安定した再現性
  • 容易なスケールアップ
  • 評価試験による特異性の確認済み
  • 倫理基準に準拠 - アニマル・フリーの生産

製品の概要

  • 製品名

    Anti-HDAC1 antibody
    HDAC1 一次抗体 製品一覧
  • 製品の詳細

    Rabbit polyclonal to HDAC1
  • 由来種

    Rabbit
  • アプリケーション

    適用あり: ICC, WB, IPmore details
  • 種交差性

    交差種: Mouse, Rat, Human, Chinese hamster
  • 免疫原

    Synthetic peptide corresponding to Human HDAC1 aa 466-482 conjugated to Keyhole Limpet Haemocyanin (KLH).
    Sequence:

    C-KEEKPEAKGVKEEVKLA


    Database link: HDAC1
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • 特記事項

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

  • 製品の状態

    Liquid
  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • バッファー

    pH: 7.40
    Preservative: 0.097% Sodium azide
    Constituent: 0.0268% PBS
  • Concentration information loading...
  • 精製度

    IgG fraction
  • 特記事項(精製)

    Whole antiserum is fractionated and further purified by anion-exchange chromatography to provide the IgG fraction of antiserum that is essentially free of other rabbit serum proteins.
  • ポリ/モノ

    ポリクローナル
  • アイソタイプ

    IgG
  • 研究分野

    • Epigenetics and Nuclear Signaling
    • Chromatin Modifying Enzymes
    • Acetylation
    • Stem Cells
    • Signaling Pathways
    • Wnt
    • HDACs
    • Epigenetics and Nuclear Signaling
    • Chromatin Modifying Enzymes
    • Acetylation
    • HDACs
    • Class I
    • Cardiovascular
    • Heart
    • Hypertrophy
    • Other
    • Isotype/Loading Controls
    • Loading Controls
    • HDAC1

関連製品

  • Assay kits

    • Histone Deacetylase (HDAC) Activity Assay Kit (Fluorometric) (ab156064)
  • ChIP Related Products

    • Rabbit Anti-Mouse IgG H&L (ab46540)
    • ChIP Kit (ab500)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Mouse monoclonal [SB62a] Anti-Rabbit IgG light chain (HRP) (ab99697)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Recombinant Protein

    • Recombinant human HDAC1 protein (ab101661)
  • Related Products

    • Prestained Protein Ladder – Broad molecular weight (10-245 kDa) (ab116028)

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab7028の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
ICC
1/200.
WB (8)
1/2000 - 1/20000. Predicted molecular weight: 55 kDa.
IP (1)
Use at an assay dependent concentration.

Use 5 -10 μL antibody per reaction

特記事項
ICC
1/200.
WB
1/2000 - 1/20000. Predicted molecular weight: 55 kDa.
IP
Use at an assay dependent concentration.

Use 5 -10 μL antibody per reaction

ターゲット情報

  • 機能

    Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Deacetylates SP proteins, SP1 and SP3, and regulates their function. Component of the BRG1-RB1-HDAC1 complex, which negatively regulates the CREST-mediated transcription in resting neurons. Upon calcium stimulation, HDAC1 is released from the complex and CREBBP is recruited, which facilitates transcriptional activation. Deacetylates TSHZ3 and regulates its transcriptional repressor activity. Deacetylates 'Lys-310' in RELA and thereby inhibits the transcriptional activity of NF-kappa-B.
  • 組織特異性

    Ubiquitous, with higher levels in heart, pancreas and testis, and lower levels in kidney and brain.
  • 配列類似性

    Belongs to the histone deacetylase family. HD type 1 subfamily.
  • 翻訳後修飾

    Sumoylated on Lys-444 and Lys-476; which promotes enzymatic activity. Desumoylated by SENP1.
    Phosphorylation on Ser-421 and Ser-423 promotes enzymatic activity and interactions with NuRD and SIN3 complexes.
    Ubiquitinated by CHFR, leading to its degradation by the proteasome.
  • 細胞内局在

    Nucleus.
  • Target information above from: UniProt accession Q13547 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 参照データベース

    • Entrez Gene: 3065 Human
    • Entrez Gene: 433759 Mouse
    • Entrez Gene: 297893 Rat
    • Omim: 601241 Human
    • SwissProt: Q13547 Human
    • SwissProt: O09106 Mouse
    • SwissProt: Q4QQW4 Rat
    • Unigene: 88556 Human
    • Unigene: 202504 Mouse
    • Unigene: 391033 Mouse
    • Unigene: 1863 Rat
    see all
  • 別名

    • DKFZp686H12203 antibody
    • GON 10 antibody
    • HD1 antibody
    • HDAC 1 antibody
    • HDAC1 antibody
    • HDAC1_HUMAN antibody
    • Histone deacetylase 1 antibody
    • Reduced potassium dependency yeast homolog like 1 antibody
    • RPD3 antibody
    • RPD3L1 antibody
    see all

画像

  • Western blot - Anti-HDAC1 antibody - ChIP Grade (ab7028)
    Western blot - Anti-HDAC1 antibody - ChIP Grade (ab7028)
    All lanes : Anti-HDAC1 antibody (ab7028) at 1/10000 dilution

    Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate
    Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate
    Lane 3 : K562 (human chronic myelogenous leukemia cell line from bone marrow ) cell lysate
    Lane 4 : CHO (Chinese hamster ovary cell line) cell lysate
    Lane 5 : NIH/3T3 (mouse embryo fibroblast cell line) cell lysate
    Lane 6 : PC-12 (rat adrenal gland pheochromocytoma cell line) cell lysate

    Secondary
    All lanes : Goat Anti-Rabbit HRP

    Predicted band size: 55 kDa

  • Immunocytochemistry - Anti-HDAC1 antibody (ab7028)
    Immunocytochemistry - Anti-HDAC1 antibody (ab7028)Hanigan, T.W. et al Send to PLoS One. 2017 Oct 18;12(10):e0186620. doi: 10.1371/journal.pone.0186620. eCollection 2017 Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/

    MCF-7 cells were treated with indicated concentrations of panobinostat (optical sections A-D, respectively) or trichostatin A (optical sections E-H, respectively) for 12 hours, fixed, permeabilized and optical sections were obtained by laser scanning confocal microscopy. Fluorescence signal for HDAC1 is shown in green (left panels), DAPI staining is shown in blue (middle panels), and merged optical sections are shown in the right panels. Colocalization analysis of HDAC1 fluorescence signal and the DAPI stain signal was performed with JACoP (ImageJ).

    HDAC1 was detected with ab7028. cells were ficed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100.

    (After Figure 3 of Hanigan et al).

  • Immunoprecipitation - Anti-HDAC1 antibody - ChIP Grade (ab7028)
    Immunoprecipitation - Anti-HDAC1 antibody - ChIP Grade (ab7028)

    HDAC1 was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract with ab7028. 

    Lane 1: 5 μl ab7028 in HeLa whole cell lysate.

    Lane 2: 10 μl ab7028 in HeLa whole cell lysate.

    Lane 3: 10 μl control antibody in HeLa whole cell lysate.

  • Immunocytochemistry - Anti-HDAC1 antibody (ab7028)
    Immunocytochemistry - Anti-HDAC1 antibody (ab7028)

    Immunofluorescence analysis of methanol fixed HeLa cells with ab7028 labelling HDAC1 at 1/200 dilution. The antibody was developed using Goat Anti-Rabbit IgG, Cy3 conjugate.

  • Western blot - Anti-HDAC1 antibody - ChIP Grade (ab7028)
    Western blot - Anti-HDAC1 antibody - ChIP Grade (ab7028)This image is courtesy of an anonymous Abreview
    All lanes : Anti-HDAC1 antibody (ab7028) at 1/1000 dilution

    Lane 1 : Whole cell lysate from human HEK293 cell line
    Lane 2 : Whole cell lysate from human HEK293 cell line treated with HDAC1 gene silencing shRNA

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : HRP-conjugated goat anti-rabbit Ig at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 55 kDa


    Exposure time: 10 seconds

    See Abreview

  • Immunoprecipitation - Anti-HDAC1 antibody - ChIP Grade (ab7028)
    Immunoprecipitation - Anti-HDAC1 antibody - ChIP Grade (ab7028)

    HDAC1 was immunoprecipitated using 0.5mg Hela whole cell extract, 5µg of Rabbit polyclonal to HDAC1 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
    The antibody was incubated under agitation with Protein G beads for 10min, Hela whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
    Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab7028.
    Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).
    Band: 60ka: HDAC1.

  • Western blot - Anti-HDAC1 antibody - ChIP Grade (ab7028)
    Western blot - Anti-HDAC1 antibody - ChIP Grade (ab7028)This image is courtesy of an anonymous Abreview
    Anti-HDAC1 antibody (ab7028) at 1/2000 dilution + Human HuH-7 whole cell lysate at 15 µg

    Secondary
    HRP conjugated Sheet anti-rabbit IgG polyclonal at 1/20000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 55 kDa


    Exposure time: 20 seconds


    Blocked with 5% milk for 1 hour at 20°C

    See Abreview

プロトコール

  • ChIP protocols
  • Immunoprecipitation protocols
  • Immunohistochemistry protocols
  • Western blot protocols

Click here to view the general protocols

データシートおよび資料

  • SDS download

  • Datasheet download

    Download

参考文献 (213)

ab7028 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab7028 は 213 報の論文で使用されています。

  • Wang Y  et al. Epigenetic regulation of intestinal peptide transporter PEPT1 as a potential strategy for colorectal cancer sensitization. Cell Death Dis 12:532 (2021). WB ; Human . PubMed: 34031358
  • Xu H  et al. Choroid plexus NKCC1 mediates cerebrospinal fluid clearance during mouse early postnatal development. Nat Commun 12:447 (2021). PubMed: 33469018
  • Kakiuchi A  et al. HDAC inhibitors suppress the proliferation, migration and invasiveness of human head and neck squamous cell carcinoma cells via p63-mediated tight junction molecules and p21-mediated growth arrest. Oncol Rep 45:N/A (2021). PubMed: 33649777
  • Polavaram NS  et al. Tumor- and osteoclast-derived NRP2 in prostate cancer bone metastases. Bone Res 9:24 (2021). PubMed: 33990538
  • Hao Y  et al. Mesenchymal Stem Cell-Derived Exosomes Carry MicroRNA-125a to Protect Against Diabetic Nephropathy by Targeting Histone Deacetylase 1 and Downregulating Endothelin-1. Diabetes Metab Syndr Obes 14:1405-1418 (2021). PubMed: 33790607
View all Publications for this product

レビューと Q&A

Show All レビュー Q&A
レビューを送る 質問を送る

1-10 of 32 Abreviews or Q&A

ChIP abreview for Anti-HDAC1 antibody - ChIP Grade

Poor
Abreviews
Abreviews
abreview image
Application
ChIP
Sample
Human Cell lysate - nuclear (MCF7 breast cancer cell line)
Negative control
Negative control qPCR primer site used
Specification
MCF7 breast cancer cell line
Detection step
Real-time PCR
Type
Cross-linking (X-ChIP)
Duration of cross-linking step: 30 minute(s) and 0 second(s)
Specification of the cross-linking agent: DSG (30 mins) + Formaldehyde (
Positive control
HDAC1 antibody from another company
Read More

DR. Sankari Nagarajan

Verified customer

投稿 Apr 04 2019

ChIP abreview for Anti-HDAC1 antibody - ChIP Grade

Average
Abreviews
Abreviews
abreview image
Application
ChIP
Sample
Mouse Tissue lysate - other (colonic crypts)
Specification
colonic crypts
Detection step
Real-time PCR
Type
Cross-linking (X-ChIP)
Duration of cross-linking step: 30 minute(s) and 0 second(s)
Specification of the cross-linking agent: formaldhyde
Read More

Abcam user community

Verified customer

投稿 Oct 06 2015

Western blot abreview for Anti-HDAC1 antibody - ChIP Grade

Good
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Mouse Cell lysate - whole cell (Cardiomyocytes)
Gel Running Conditions
Reduced Denaturing
Loading amount
25 µg
Specification
Cardiomyocytes
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Read More

Abcam user community

Verified customer

投稿 Sep 28 2015

Western blot abreview for Anti-HDAC1 antibody - ChIP Grade

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Loading amount
10000 cells
Gel Running Conditions
Reduced Denaturing (4-12% gradient)
Sample
Human Cell lysate - whole cell (Huh7 liver)
Specification
Huh7 liver
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Read More

Abcam user community

Verified customer

投稿 Jan 26 2015

Western blot abreview for Anti-HDAC1 antibody - ChIP Grade

Excellent
Abreviews
Abreviews
Application
Western blot
Sample
Mouse Tissue lysate - whole (Forebrain Tissue)
Loading amount
200 µg
Specification
Forebrain Tissue
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Read More

Abcam user community

Verified customer

投稿 Nov 07 2012

Western blot abreview for Anti-HDAC1 antibody - ChIP Grade

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (huh-7 cell line)
Loading amount
15 µg
Specification
huh-7 cell line
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Read More

Abcam user community

Verified customer

投稿 May 04 2012

Question

I would like to know the exact concentration of these antibodies, since in the datasheet it is not mentioned:
Anti-HDAC1 antibody - ChIP Grade (ab7028)
Anti-BAF53A antibody (ab84486).

Read More

Abcam community

Verified customer

Asked on Jan 31 2013

Answer

The antibody concentrations are:
Anti-HDAC1 antibody - ChIP Grade (ab7028), lot GR9959 - 0.2 mg/ml
Anti-BAF53A antibody (ab84486), lot GR41407 - 10.0 mg/ml.

Read More

Abcam Scientific Support

Answered on Jan 31 2013

Question

The order number this item came from is XXX...If there is going to be a replacement please use all the same shipping information from the original order....


Thank you

Read More

Abcam community

Verified customer

Asked on Aug 30 2012

Answer

Thank you for your reply.


I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement.

To check the status of the order please contact our Customer Service team and reference this number.

Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know.

I wish you the best of luck with your research.

Read More

Abcam Scientific Support

Answered on Aug 30 2012

Question

My lab has previously used ab7028 (Rabbit HDAC1) for ChIP analysis and obtained excellent results. This newer lot is giving us very poor results with very little enrichment over the IgG negative control. We would like to find out if there is a problem with the lot and request a replacement.

Read More

Abcam community

Verified customer

Asked on Aug 28 2012

Answer

Thank you for contacting Abcam regarding ab7028.


I am sorry that you have been experiencing difficutlies with this lot of antibody in ChIP. We do have another lot available that I will be happy to send as a replacement. Can you please provide your order number for this antibody?


Additionally, we would like to investigate this further in our laboratory. Can you please provide a summary of the protocol you followed? This will help us to identify any potential problems with this particular lot.


I look forward to your reply so that I may assist you further. Please do not hesitate to contact me if you have any additional questions or concerns.

Read More

Abcam Scientific Support

Answered on Aug 28 2012

Question

This is the customer who inquired about the HDAC1 antibody. I would like to inform you that we are now having success with the new antibody you sent us. It is working for both IF and WB with our cells and lysates. Thank you for the assistance once again. Abcam customer,

Read More

Abcam community

Verified customer

Asked on Dec 05 2011

Answer

Thank you so much for keeping me updated about the situation, and I am so happy to hear that the replacement from a new lot is working well. Please let me know if there is anything else that you need, and have a great week!  

Read More

Abcam Scientific Support

Answered on Dec 05 2011

1-10 of 32 Abreviews or Q&A

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