Application
Western blot
Sample
Human Cell lysate - nuclear (HeLa cells)
Loading amount
10 µg
Specification
HeLa cells
Gel Running Conditions
Reduced Denaturing (7)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Other product details
Dilution
1/500
Incubation time
24 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% BSA in 1X PBS + 0.1% Tween-20
Secondary antibody
Name
Non-Abcam antibody was used: ECL Anti-rabbit IgG, Horseradish Peroxidase linked
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/5000
Detection
Detection method
ECL
Exposure
30 second(s)
Bands
Specific: 70 kDa Non-specific: 35 kDa
Additional data
Additional Notes
The same HeLa nuclear extract sample was loaded in each lane with Lanes 1, 2, and 3 each containing approximately 10, 5, and 2.5 ug of protein, respectively.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Mar 08 2008