Anti-HCN2 抗体 [S71] (ab84817)
Key features and details
- Mouse monoclonal [S71] to HCN2
- Suitable for: IHC-Fr, IHC-P, ICC, WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG1
製品の概要
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製品名
Anti-HCN2 antibody [S71]
HCN2 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [S71] to HCN2 -
由来種
Mouse -
特異性
Does not cross react with HCN1. -
アプリケーション
適用あり: IHC-Fr, IHC-P, ICC, WBmore details -
種交差性
交差種: Mouse, Rat, Human -
免疫原
Fusion protein corresponding to Rat HCN2 aa 750 to the C-terminus (C terminal).
Database link: Q9JKA9 -
ポジティブ・コントロール
- IHC-Fr: Mouse brain tissue; IHC-P: Human hippocampus tissue; WB: Rat brain membrane and mouse brain tissue lysates; ICC: Differentiated SH-SH5Y cells.
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特記事項
The clone number S71 has previously been called S71-37 and N71/37. These clone numbers all refer to the same antibody clone.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
バッファー
Preservative: 0.09% Sodium azide
Constituents: 50% Glycerol, PBS -
Concentration information loading...
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精製度
Protein G purified -
ポリ/モノ
モノクローナル -
クローン名
S71 -
アイソタイプ
IgG1 -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab84817の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
IHC-Fr |
1/1000.
|
|
IHC-P |
1/100.
|
|
ICC |
1/100.
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|
WB | (3) |
1/1000. Predicted molecular weight: 95 kDa.
|
特記事項 |
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IHC-Fr
1/1000. |
IHC-P
1/100. |
ICC
1/100. |
WB
1/1000. Predicted molecular weight: 95 kDa. |
ターゲット情報
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機能
Hyperpolarization-activated ion channel exhibiting weak selectivity for potassium over sodium ions. Contributes to the native pacemaker currents in heart (If) and in neurons (Ih). Produces a large instantaneous current. Activated by cAMP. Modulated by intracellular chloride ions and pH; acidic pH shifts the activation to more negative voltages. -
組織特異性
Highly expressed throughout the brain. Detected at low levels in heart. -
配列類似性
Belongs to the potassium channel HCN family.
Contains 1 cyclic nucleotide-binding domain. -
ドメイン
The segment S4 is probably the voltage-sensor and is characterized by a series of positively charged amino acids at every third position. -
細胞内局在
Membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 610 Human
- Entrez Gene: 15166 Mouse
- Entrez Gene: 114244 Rat
- Omim: 602781 Human
- SwissProt: Q9UL51 Human
- SwissProt: O88703 Mouse
- SwissProt: Q9JKA9 Rat
- Unigene: 124161 Human
see all -
別名
- BCNG-2 antibody
- BCNG2 antibody
- Brain cyclic nucleotide gated channel 2 antibody
see all
画像
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Immunocytochemistry analysis of differentiated SH-SY5Y (human neuroblastoma cell line from bone marrow) cells labelling HCN2 with ab84817 at 1/100 dilution followed by secondary antibody (Alexa Fluor® 488) (green). Cells were counterstained with phalloidin (Alexa Fluor® 647) (red), and with Anti-beta III Tubulin antibody (Alexa Fluor® 555) (magenta). Nuclear DNA was labelled with Hoechst (blue).
(A) Phalloidin
(B) Anti-beta III Tubulin antibody
(C) HCN2 Antibody (ab84817) and secondary antibody
(D) Hoechst
(E) Composite
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Immunohistochemistry (Bouin-fixed paraffin-embedded sections) analysis of human hippocampus tissue labelling HCN2 with ab84817 at 1/100 dilution (1 hour at room temperature). FITC Goat Anti-Mouse (green) was used as the secondary antibody at 1/50 dilution (1 hour at room temperature).
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Immunohistochemistry (Frozen sections) analysis of mouse brain tissue labelling HCN2 with ab84817 at 1/1000 dilution (1 hour at room temperature). Tissue fixed with 10% Formalin Solution for 12-24 hours at room temperature. Secondary Antibody: HRP/DAB Detection System: Biotinylated Goat Anti-Mouse, Streptavidin Peroxidase, DAB Chromogen (brown) for 30 minutes at room temperature. Counterstained with Mayer hematoxylin (purple/blue) nuclear stain at 250-500 µL for 5 minutes at room temperature.
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Anti-HCN2 antibody [S71] (ab84817) at 1/1000 dilution + Mouse brain tissue lysate at 15 µg
Secondary
Goat anti-mouse IgG, HRP at 1/4000 dilution
Predicted band size: 95 kDa
Observed band size: 95 kDaChemiluminescent for HRP (Moss) for 5 min in RT.
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Anti-HCN2 antibody [S71] (ab84817) at 1/1000 dilution + Rat brain membrane lysates at 15 µg
Secondary
HRP-Sheep Anti-Mouse IgG antibody
Predicted band size: 95 kDa
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (10)
ab84817 は 10 報の論文で使用されています。
- Lei X et al. Activation of HCN channels caused by elevated cAMP levels in periaqueductal gray promotes bone cancer pain. Neurochem Int 162:105437 (2023). PubMed: 36336089
- Zhong W & Darmani NA The HCN Channel Blocker ZD7288 Induces Emesis in the Least Shrew (Cryptotis parva). Front Pharmacol 12:647021 (2021). PubMed: 33995059
- Zhang X et al. Shank3 contributes to neuropathic pain by facilitating the SNI-dependent increase of HCN2 and the expression of PSD95. Neurosci Res N/A:N/A (2020). PubMed: 32454040
- Zhao H et al. Overexpression of the medium-conductance calcium-activated potassium channel (SK4) and the HCN2 channel to generate a biological pacemaker. Mol Med Rep 20:3406-3414 (2019). PubMed: 31432175
- Mader F et al. Hyperpolarization-Activated Cyclic Nucleotide-Gated Non-selective (HCN) Ion Channels Regulate Human and Murine Urinary Bladder Contractility. Front Physiol 9:753 (2018). PubMed: 29971015
- Ding W et al. Increased HCN Channel Activity in the Gasserian Ganglion Contributes to Trigeminal Neuropathic Pain. J Pain 19:626-634 (2018). PubMed: 29366880
- Guo T et al. Association between hyperpolarization-activated channel in interstitial cells of Cajal and gastrointestinal dysmotility induced by malignant ascites. Oncol Lett 13:1601-1608 (2017). IHC-Fr ; Mouse . PubMed: 28454297
- Pitcairn E et al. Coordinating heart morphogenesis: A novel role for hyperpolarization-activated cyclic nucleotide-gated (HCN) channels during cardiogenesis inXenopus laevis. Commun Integr Biol 10:e1309488 (2017). PubMed: 28702127
- Ding W et al. Inhibition of HCN channel activity in the thalamus attenuates chronic pain in rats. Neurosci Lett 631:97-103 (2016). PubMed: 27542339
- Feng QF et al. The effects of acupuncture on bladder interstitial cells of cajal excitability in rats with overactive bladder. Evid Based Complement Alternat Med 2013:261217 (2013). IHC . PubMed: 24194780