Anti-hCG 抗体 (ab9376)
Key features and details
- Rabbit polyclonal to hCG
- Suitable for: IHC-P, IHC-Fr, WB
- Reacts with: Human
- Isotype: IgG
製品の概要
-
製品名
Anti-hCG antibody
hCG 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to hCG -
由来種
Rabbit -
特異性
This antibody reacts with hCG. It stains strongly with the human placenta and placental trophoblasts. No non-specific stainingwith any other tissue has been observed. -
アプリケーション
適用あり: IHC-P, IHC-Fr, WBmore details -
種交差性
交差種: Human -
免疫原
Beta chain of human chorionic gonadotropin.
-
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.3 -
Concentration information loading... -
精製度
Protein A purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
-
Compatible Secondaries
-
Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab9376の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IHC-P |
Use at an assay dependent concentration.
This antibody may be diluted to a titer of 1:100-1:500 in an ABC method. Staining Protocol: We suggest an incubation period of 30-60 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation conditions and antibody dilutions should be determined by the user. Although not required, proteolytic treatment may enhance the immunostaining in overfixed tissues. |
|
| IHC-Fr |
Use at an assay dependent concentration.
|
|
| WB | (1) |
Use at an assay dependent concentration. Predicted molecular weight: 18 kDa.
|
| 特記事項 |
|---|
|
IHC-P
Use at an assay dependent concentration. This antibody may be diluted to a titer of 1:100-1:500 in an ABC method. Staining Protocol: We suggest an incubation period of 30-60 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation conditions and antibody dilutions should be determined by the user. Although not required, proteolytic treatment may enhance the immunostaining in overfixed tissues. |
|
IHC-Fr
Use at an assay dependent concentration. |
|
WB
Use at an assay dependent concentration. Predicted molecular weight: 18 kDa. |
ターゲット情報
-
関連性
Human chorionic gonadotropin (hCG) is a glycoprotein hormone produced by trophoblastic cells of the placenta beginning 10 to 12 days after conception. Maintenance of the fetus in the first trimester of pregnancy requires the production of hCG, which binds to the corpus luteum of the ovary which is stimulated to produce progesterone which in turn maintains the secretory endometrium. hCG is present only in trace amounts in non pregnant urine and sera. It rises sharply during pregnancy. HCG is composed of two non identical, non covalently linked polypeptide chains designated as the a and b subunits. The a subunit of HCG is nearly identical to that of thyroid stimulating hormone (TSH), follicle stimulating hormone (FSH) and luteinizing hormone (LH). A germ cell tumor which is positive for cytokeratin, placental alkaline phosphatase (PLAP) and HCG but negative for EMA and AFP is probably a choriocarcinoma. -
細胞内局在
Secreted -
参照データベース
- Entrez Gene: 1081 Human
- Entrez Gene: 1082 Human
- Omim: 118850 Human
- Omim: 118860 Human
- SwissProt: P01215 Human
- SwissProt: P01233 Human
-
別名
- anterior pituitary glycoprotein hormones common subunit alpha antibody
- CG alpha antibody
- CG-ALPHA antibody
see all
画像
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-hCG antibody (ab9376)Human normal placenta. Staining is localised extracellular and in the cytoplasm. Left panel: with primary antibody at 2 ug/ml. Right panel: isotype control. Sections were stained using an automated system DAKO Autostainer Plus , at room temperature: sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffers citrate EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for rabbit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-hCG antibody (ab9376)ab9376 staining hCG in formalin-fixed, paraffin-embedded Human placenta by Immunohistochemistry.
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (6)
ab9376 は 6 報の論文で使用されています。
- Azar C et al. RNA-Seq identifies genes whose proteins are upregulated during syncytia development in murine C2C12 myoblasts and human BeWo trophoblasts. Physiol Rep 9:e14671 (2021). PubMed: 33403800
- Kojima J et al. Efficient production of trophoblast lineage cells from human induced pluripotent stem cells. Lab Invest 97:1188-1200 (2017). PubMed: 28287635
- Peng B et al. Gonadotropin-releasing hormone and gonadotropin-releasing hormone receptor are expressed at tubal ectopic pregnancy implantation sites. Fertil Steril 105:1620-1627.e3 (2016). PubMed: 26920257
- Wang JM et al. The human leukocyte antigen G promotes trophoblast fusion and ß-hCG production through the Erk1/2 pathway in human choriocarcinoma cell lines. Biochem Biophys Res Commun 434:460-5 (2013). PubMed: 23583402
- Dukic-Stefanovic S et al. Chorionic gonadotropin and its receptor are both expressed in human retina, possible implications in normal and pathological conditions. PLoS One 7:e52567 (2012). IHC-Fr ; Human . PubMed: 23285091
- Kane N et al. Proliferation of uterine natural killer cells is induced by human chorionic gonadotropin and mediated via the mannose receptor. Endocrinology 150:2882-8 (2009). ICC/IF ; Human . PubMed: 19196802