Application
IHC - Wholemount
Sample
Mouse Tissue (Olfactory Bulb)
Specification
Olfactory Bulb
Other product details
Incubation time
18 hour(s) and 0 minute(s)
Dilution
1/200
Secondary antibody
Dilution
1/750
Name
Non-Abcam antibody was used: donkey anti-rabbit
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Additional data
Additional Notes
The tissue was fixed with PFA. During immuhistochemistry the tissue was blocked with 5% NDS. Under the confocal microscope, the antibody did not show specific staining.
Abcam response
Thank you so much for testing this antibody in IHC whole mount and mouse.
We appreciate very much your efforts, but are sorry to hear that the antibody did not work well in this application and species. The customer received a discount on a future antibody purchase for testing this product in IHC whole mount and mouse. We would welcome additional feedback from other customers as well.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
投稿 Jun 17 2013