Anti-HAUSP / USP7 抗体 [EPR4253] (ab108931)
![Western blot - Anti-HAUSP / USP7 antibody [EPR4253] (ab108931)](https://www.abcam.co.jp/ps/products/108/ab108931/Images/ab108931-256361-anti-hausp-usp7-antibody-epr4253-western-blot.jpg "Western blot - Anti-HAUSP / USP7 antibody [EPR4253] (ab108931)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR4253] to HAUSP / USP7
- Suitable for: Flow Cyt (Intra), WB, IHC-P, ICC/IF
- Knockout validated
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
製品の概要
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製品名
Anti-HAUSP / USP7 antibody [EPR4253]
HAUSP / USP7 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR4253] to HAUSP / USP7 -
由来種
Rabbit -
アプリケーション
適用あり: Flow Cyt (Intra), WB, IHC-P, ICC/IFmore details
適用なし: IP -
種交差性
交差種: Mouse, Rat, Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ･コントロール
- WB: HEK293T, Ramos, HeLa, A431, MCF7, C6 and RAW 264.7 cell lysates. IHC-P: Human colon tissue. IF: HeLa cells. Flow Cyt (intra): HeLa cells.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
バッファー
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR4253 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Compatible Secondaries
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Isotype control
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KO cell lines
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KO cell lysates
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab108931の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| Flow Cyt (Intra) |
Use at an assay dependent concentration.
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| WB | (1) |
1/1000 - 1/10000. Predicted molecular weight: 128 kDa.
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| IHC-P |
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Antigen retrieval is recommended. |
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| ICC/IF | (1) |
1/100 - 1/250.
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| 特記事項 |
|---|
|
Flow Cyt (Intra)
Use at an assay dependent concentration. |
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WB
1/1000 - 1/10000. Predicted molecular weight: 128 kDa. |
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IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Antigen retrieval is recommended. |
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ICC/IF
1/100 - 1/250. |
ターゲット情報
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機能
Hydrolase that deubiquitinates target proteins such as FOXO4, p53/TP53, MDM2, ERCC6, DNMT1, UHRF1, PTEN and DAXX (PubMed:11923872, PubMed:15053880, PubMed:16964248, PubMed:18716620, PubMed:25283148). Together with DAXX, prevents MDM2 self-ubiquitination and enhances the E3 ligase activity of MDM2 towards p53/TP53, thereby promoting p53/TP53 ubiquitination and proteasomal degradation. Deubiquitinates p53/TP53, preventing degradation of p53/TP53, and enhances p53/TP53-dependent transcription regulation, cell growth repression and apoptosis (PubMed:25283148). Deubiquitinates p53/TP53 and MDM2 and strongly stabilizes p53/TP53 even in the presence of excess MDM2, and also induces p53/TP53-dependent cell growth repression and apoptosis. Deubiquitination of FOXO4 in presence of hydrogen peroxide is not dependent on p53/TP53 and inhibits FOXO4-induced transcriptional activity. In association with DAXX, is involved in the deubiquitination and translocation of PTEN from the nucleus to the cytoplasm, both processes that are counteracted by PML. Involved in cell proliferation during early embryonic development. Involved in transcription-coupled nucleotide excision repair (TC-NER) in response to UV damage: recruited to DNA damage sites following interaction with KIAA1530/UVSSA and promotes deubiquitination of ERCC6, preventing UV-induced degradation of ERCC6. Contributes to the overall stabilization and trans-activation capability of the herpesvirus 1 trans-acting transcriptional protein ICP0/VMW110 during HSV-1 infection. Involved in maintenance of DNA methylation via its interaction with UHRF1 and DNMT1: acts by mediating deubiquitination of UHRF1 and DNMT1, preventing their degradation and promoting DNA methylation by DNMT1 (PubMed:21745816). Exhibits a preference towards 'Lys-48'-linked ubiquitin chains. Increases regulatory T-cells (Treg) suppressive capacity by deubiquitinating and stabilizing the transcription factor FOXP3 which is crucial for Treg cell function (PubMed:23973222). -
組織特異性
Widely expressed. Overexpressed in prostate cancer. -
配列類似性
Belongs to the peptidase C19 family.
Contains 1 MATH domain.
Contains 1 USP domain. -
ドメイン
The C-terminus plays a role in its oligomerization. -
翻訳後修飾
Isoform 1: Phosphorylated. Isoform 1 is phosphorylated at positions Ser-18 and Ser-963. Isoform 2: Not phosphorylated.
Isoform 1: Polyneddylated. Isoform 2: Not Polyneddylated.
Isoform 1 and isoform 2: Not sumoylated.
Isoform 1 and isoform 2: Polyubiquitinated by herpesvirus 1 trans-acting transcriptional protein ICP0/VMW110; leading to its subsequent proteasomal degradation. Isoform 1: Ubiquitinated at Lys-869. -
細胞内局在
Nucleus. Cytoplasm. Nucleus, PML body. Present in a minority of ND10 nuclear bodies. Association with ICP0/VMW110 at early times of infection leads to an increased proportion of USP7-containing ND10. Colocalizes with ATXN1 in the nucleus. Colocalized with DAXX in speckled structures. Colocalized with PML and PTEN in promyelocytic leukemia protein (PML) nuclear bodies. - Information by UniProt
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参照データベース
- Entrez Gene: 7874 Human
- Entrez Gene: 252870 Mouse
- Entrez Gene: 360471 Rat
- Omim: 602519 Human
- SwissProt: Q93009 Human
- SwissProt: Q6A4J8 Mouse
- SwissProt: Q4VSI4 Rat
- Unigene: 386939 Human
see all -
別名
- Deubiquitinating enzyme 7 antibody
- HAUSP antibody
- Herpes virus associated ubiquitin specific protease antibody
see all
画像
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Western blot - Anti-HAUSP / USP7 antibody [EPR4253] (ab108931)
Lane 1: Wild-type HAP1 cell lysate (20 µg)
Lane 2: HAUSP/USP7 knockout HAP1 cell lysate (20 µg)
Lane 3: HeLa cell lysate (20 µg)
Lane 4: MCF7 cell lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab108931 observed at 115 kDa. Red - loading control, ab8245, observed at 37 kDa.ab108931 was shown to specifically react with HAUSP/USP7 when HAUSP/USP7 knockout samples were used. Wild-type and HAUSP/USP7 knockout samples were subjected to SDS-PAGE. ab108931 and ab8245 (loading control to GAPDH) were diluted 1/1000 and 1/2000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10 000 dilution for 1 h at room temperature before imaging.
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![Flow Cytometry (Intracellular) - Anti-HAUSP / USP7 antibody [EPR4253] (ab108931)](https://www.abcam.co.jp/ps/products/108/ab108931/Images/ab108931-276878-anti-hausp-usp7-antibody-epr4253-flow-cytometry.jpg)
Flow Cytometry (Intracellular) - Anti-HAUSP / USP7 antibody [EPR4253] (ab108931)Intracellular Flow Cytometry analysis of HeLa (human cervix adenocarcinoma) cells labeling HAUSP / USP7 with unpurified ab108931 at 1/30 dilution (10ug/ml) (red). Cells were fixed with 80% methanol and permeabilised with 0.1% Tween-20. A Goat anti rabbit IgG (Alexa Fluor® 488) (ab150077) (1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black) (ab172730) was used as the isotype control, Cell without incubation with primary antibody and secondary antibody (Blue) were used as the unlabeled control.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HAUSP / USP7 antibody [EPR4253] (ab108931)](https://www.abcam.co.jp/ps/products/108/ab108931/Images/ab108931-121425-anti-hausp-usp7-antibody-epr4253-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HAUSP / USP7 antibody [EPR4253] (ab108931)ab108931, at 1/50, staining HAUSP / USP7 in Human colon tissue by Immunohistochemistry, Paraffin-embedded tissue.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Western blot - Anti-HAUSP / USP7 antibody [EPR4253] (ab108931)](https://www.abcam.co.jp/ps/products/108/ab108931/Images/ab108931-409572-anti-hausp-usp7-antibody-epr4253-western-blot-edited-hek293t.jpg)
Western blot - Anti-HAUSP / USP7 antibody [EPR4253] (ab108931)All lanes : Anti-HAUSP / USP7 antibody [EPR4253] (ab108931) at 1/1000 dilution
Lane 1 : Wild-type HEK-293T cell lysate
Lane 2 : USP7 CRISPR/Cas9 edited HEK-293T cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 128 kDa
Observed band size: 128 kDaLanes 1- 2: Merged signal (red and green). Green - ab108931 observed at 128 kDa. Red - Anti-GAPDH antibody [6C5] - Loading Control (ab8245) observed at 37 kDa.
ab108931 was shown to react with HAUSP / USP7 in wild-type HEK-293T cells in western blot. The band observed in CRISPR/Cas9 edited cell line ab266535 (CRISPR/Cas9 edited cell lysate ab257284) lane below 128kDa may represent truncated forms and cleaved fragments. This has not been investigated further. Wild-type HEK-293T and USP7 CRISPR/Cas9 edited HEK-293T cell lysates were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab108931 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye®800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye®680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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![Western blot - Anti-HAUSP / USP7 antibody [EPR4253] (ab108931)](https://www.abcam.co.jp/ps/products/108/ab108931/Images/ab108931-121426-anti-hausp-usp7-antibody-epr4253-western-blot.gif)
Western blot - Anti-HAUSP / USP7 antibody [EPR4253] (ab108931)All lanes : Anti-HAUSP / USP7 antibody [EPR4253] (ab108931) at 1/1000 dilution
Lane 1 : Ramos cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : A431 cell lysate
Lane 4 : MCF7 cell lysate
Lane 5 : C6 cell lysate
Lane 6 : RAW 264.7 cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 128 kDa -
![Immunocytochemistry/ Immunofluorescence - Anti-HAUSP / USP7 antibody [EPR4253] (ab108931)](https://www.abcam.co.jp/ps/products/108/ab108931/Images/ab108931-121424-anti-hausp-usp7-antibody-epr4253-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-HAUSP / USP7 antibody [EPR4253] (ab108931)ab108931, at 1/100, staining HAUSP / USP7 in HeLa cells by Immunofluorescence. -
![Anti-HAUSP / USP7 antibody [EPR4253] (ab108931)](https://www.abcam.co.jp/ps/products/108/ab108931/Images/ab108931-7-benefits-of-recombinant-antibodies.png)
Anti-HAUSP / USP7 antibody [EPR4253] (ab108931)
プロトコール
データシートおよび資料
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Datasheet download
参考文献 (3)
ab108931 は 3 報の論文で使用されています。
- Liu J et al. Arginine methylation-dependent LSD1 stability promotes invasion and metastasis of breast cancer. EMBO Rep 21:e48597 (2020). PubMed: 31833203
- Hu T et al. Targeting the overexpressed USP7 inhibits esophageal squamous cell carcinoma cell growth by inducing NOXA-mediated apoptosis. Mol Carcinog 58:42-54 (2019). PubMed: 30182448
- Xia X et al. Deubiquitination and stabilization of estrogen receptor a by ubiquitin-specific protease 7 promotes breast tumorigenesis. Cancer Lett 465:118-128 (2019). PubMed: 31518603
