Anti-HAUSP / USP7 抗体 (ab4080)
Key features and details
- Rabbit polyclonal to HAUSP / USP7
- Suitable for: IHC-P, IP, ELISA, WB, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-HAUSP / USP7 antibody
HAUSP / USP7 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to HAUSP / USP7 -
由来種
Rabbit -
特異性
By Western blot, this antibody detects a single clean band of about 130kD representing HAUSP in various cell lysates (see picture). The band is strongly depleted using peptide blocking with the immunising peptide. -
アプリケーション
適用あり: IHC-P, IP, ELISA, WB, ICC/IFmore details -
種交差性
交差種: Mouse, Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
Preservative: 0.01% Sodium azide
Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab4080の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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IHC-P | (1) |
Use at an assay dependent concentration. PubMed: 16450335
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IP | (1) |
Use at an assay dependent concentration.
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ELISA |
1/15000 - 1/60000.
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WB | (1) |
1/500 - 1/2000. Detects a band of approximately 130 kDa (predicted molecular weight: 128 kDa).
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ICC/IF |
Use a concentration of 1 µg/ml.
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特記事項 |
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IHC-P
Use at an assay dependent concentration. PubMed: 16450335 |
IP
Use at an assay dependent concentration. |
ELISA
1/15000 - 1/60000. |
WB
1/500 - 1/2000. Detects a band of approximately 130 kDa (predicted molecular weight: 128 kDa). |
ICC/IF
Use a concentration of 1 µg/ml. |
ターゲット情報
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機能
Hydrolase that deubiquitinates target proteins such as FOXO4, p53/TP53, MDM2, ERCC6, DNMT1, UHRF1, PTEN and DAXX (PubMed:11923872, PubMed:15053880, PubMed:16964248, PubMed:18716620, PubMed:25283148). Together with DAXX, prevents MDM2 self-ubiquitination and enhances the E3 ligase activity of MDM2 towards p53/TP53, thereby promoting p53/TP53 ubiquitination and proteasomal degradation. Deubiquitinates p53/TP53, preventing degradation of p53/TP53, and enhances p53/TP53-dependent transcription regulation, cell growth repression and apoptosis (PubMed:25283148). Deubiquitinates p53/TP53 and MDM2 and strongly stabilizes p53/TP53 even in the presence of excess MDM2, and also induces p53/TP53-dependent cell growth repression and apoptosis. Deubiquitination of FOXO4 in presence of hydrogen peroxide is not dependent on p53/TP53 and inhibits FOXO4-induced transcriptional activity. In association with DAXX, is involved in the deubiquitination and translocation of PTEN from the nucleus to the cytoplasm, both processes that are counteracted by PML. Involved in cell proliferation during early embryonic development. Involved in transcription-coupled nucleotide excision repair (TC-NER) in response to UV damage: recruited to DNA damage sites following interaction with KIAA1530/UVSSA and promotes deubiquitination of ERCC6, preventing UV-induced degradation of ERCC6. Contributes to the overall stabilization and trans-activation capability of the herpesvirus 1 trans-acting transcriptional protein ICP0/VMW110 during HSV-1 infection. Involved in maintenance of DNA methylation via its interaction with UHRF1 and DNMT1: acts by mediating deubiquitination of UHRF1 and DNMT1, preventing their degradation and promoting DNA methylation by DNMT1 (PubMed:21745816). Exhibits a preference towards 'Lys-48'-linked ubiquitin chains. Increases regulatory T-cells (Treg) suppressive capacity by deubiquitinating and stabilizing the transcription factor FOXP3 which is crucial for Treg cell function (PubMed:23973222). -
組織特異性
Widely expressed. Overexpressed in prostate cancer. -
配列類似性
Belongs to the peptidase C19 family.
Contains 1 MATH domain.
Contains 1 USP domain. -
ドメイン
The C-terminus plays a role in its oligomerization. -
翻訳後修飾
Isoform 1: Phosphorylated. Isoform 1 is phosphorylated at positions Ser-18 and Ser-963. Isoform 2: Not phosphorylated.
Isoform 1: Polyneddylated. Isoform 2: Not Polyneddylated.
Isoform 1 and isoform 2: Not sumoylated.
Isoform 1 and isoform 2: Polyubiquitinated by herpesvirus 1 trans-acting transcriptional protein ICP0/VMW110; leading to its subsequent proteasomal degradation. Isoform 1: Ubiquitinated at Lys-869. -
細胞内局在
Nucleus. Cytoplasm. Nucleus, PML body. Present in a minority of ND10 nuclear bodies. Association with ICP0/VMW110 at early times of infection leads to an increased proportion of USP7-containing ND10. Colocalizes with ATXN1 in the nucleus. Colocalized with DAXX in speckled structures. Colocalized with PML and PTEN in promyelocytic leukemia protein (PML) nuclear bodies. - Information by UniProt
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参照データベース
- Entrez Gene: 7874 Human
- Entrez Gene: 252870 Mouse
- Omim: 602519 Human
- SwissProt: Q93009 Human
- SwissProt: Q6A4J8 Mouse
- Unigene: 386939 Human
- Unigene: 295330 Mouse
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別名
- Deubiquitinating enzyme 7 antibody
- HAUSP antibody
- Herpes virus associated ubiquitin specific protease antibody
see all
画像
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Western blot using ab4080 at 1/500. Secondary antibody anti-rabbit HRP ab6721 at 1/5000. Exposure time 30s.
Lane 1: Hela nuclear
Lane 2: Hela
Lane 3: A431
Lane 4: MCF7
Lane 5: 3T3
Lane 6: Hela nuclear + ab11707 HAUSP peptide (aa30-41)
Lane 7: Hela + ab11707 HAUSP peptide (aa30-41)
Lane 8: A431 + ab11707 HAUSP peptide (aa30-41)
Lane 9: MCF7 + ab11707 HAUSP peptide (aa30-41)
Lane 10: 3T3 + ab11707 HAUSP peptide (aa30-41)Western blot using ab4080 at 1/500. Secondary ab6721 at 1/5000. Exposure time 30s.
Lane 1: Hela nuclear
Lane 2: Hela
Lane 3: A431
Lane 4: MCF7
Lane 5: 3T3
Lane 6: Hela nuclear + ab11707 HAUSP peptide (aa30-41)
Lane 7: Hela + ab11707 HAUSP peptide (aa30-41)
Lane 8: A431 + ab11707 HAUSP peptide (aa30-41)
Lane 9: MCF7 + ab11707 HAUSP peptide (aa30-41)
Lane 10: 3T3 + ab11707 HAUSP peptide (aa30-41) -
ICC/IF image of ab4080 stained Mcf7 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab4080, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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ELISA results of purified ab4080 tested against BSA-conjugated peptide of immunizing peptide. Each well was coated in duplicate with 0.1 µg of conjugate. The starting dilution of antibody was 5 µg/mL and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using 3% fish gelatin as blocking buffer, Goat anti-Rabbit IgG Peroxidase Conjugated secondary antibody and TMB substrate.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (33)
ab4080 は 33 報の論文で使用されています。
- Molkentine DP et al. p16 Represses DNA Damage Repair via a Novel Ubiquitin-Dependent Signaling Cascade. Cancer Res 82:916-928 (2022). PubMed: 34965932
- Schaefer EJ et al. BCOR and BCORL1 Mutations Drive Epigenetic Reprogramming and Oncogenic Signaling by Unlinking PRC1.1 from Target Genes. Blood Cancer Discov 3:116-135 (2022). PubMed: 35015684
- Saha S et al. Proteomic analysis reveals USP7 as a novel regulator of palmitic acid-induced hepatocellular carcinoma cell death. Cell Death Dis 13:563 (2022). PubMed: 35732625
- Yi J et al. Parthenolide and arsenic trioxide co-trigger autophagy-accompanied apoptosis in hepatocellular carcinoma cells. Front Oncol 12:988528 (2022). PubMed: 36353537
- Xiang M et al. Pharmacological inhibition of USP7 suppresses growth and metastasis of melanoma cells in vitro and in vivo. J Cell Mol Med 25:9228-9240 (2021). PubMed: 34469054