Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Lung, lung cancer)
Specification
Lung, lung cancer
Fixative
HOPE (Hepes-glutamic acid buffer mediated Organic solvent Protection Effect Olert et al.,2001 Pathol Res Pract)
Antigen retrieval step
None
Permeabilization
No
Blocking step
Zytomed Systems Blocking Solution as blocking agent for 5 minute(s) · Concentration: 100% · Temperature: 25°C
Other product details
Dilution
1/100
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 25°C · Diluent: Zytomed Systems Antibody Diluent
Secondary antibody
Name
Non-Abcam antibody was used: One Step Polymer System (Zytomed Systems)
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Additional data
PubMed ID
21240070: View
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
投稿 Nov 03 2011