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Anti-Haptoglobin 抗体 [HG-36] (ab13429)

Reviews (10)Q&A (5)References (17)

Key features and details

  • Mouse monoclonal [HG-36] to Haptoglobin
  • Suitable for: ELISA, Dot blot, ICC/IF, WB, IHC-P
  • Reacts with: Human
  • Isotype: IgG1

製品の概要

  • 製品名

    Anti-Haptoglobin antibody [HG-36]
    Haptoglobin 一次抗体 製品一覧

  • 製品の詳細

    Mouse monoclonal [HG-36] to Haptoglobin

  • 由来種

    Mouse

  • アプリケーション

    適用あり: ELISA, Dot blot, ICC/IF, WB, IHC-Pmore details

  • 種交差性

    交差種: Human

  • 免疫原

    Full length native protein (Purified haptoglobin types 1-1, 2-1, and 2-2 from pooled human plasma).

  • 特記事項

    This product was changed from ascites to tissue culture supernatant on 17 May 2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

  • バッファー

    pH: 7.40
    Preservative: 0.098% Sodium azide
    Constituent: 99% PBS
  • Concentration information loading...

  • 精製度

    Proprietary Purification

  • 特記事項（精製）

    Purified from hybridoma cell culture

  • ポリ/モノ

    モノクローナル

  • クローン名

    HG-36

  • アイソタイプ

    IgG1

  • 研究分野

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab13429の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
ELISA
Use at an assay dependent concentration.
Dot blot
Use at an assay dependent concentration.
ICC/IF (2)
Use at an assay dependent concentration.
WB (2)
Use at an assay dependent concentration. Predicted molecular weight: 45 kDa.
IHC-P (4)
Use at an assay dependent concentration.

See Abreview.
特記事項
ELISA
Use at an assay dependent concentration.
Dot blot
Use at an assay dependent concentration.
ICC/IF
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration. Predicted molecular weight: 45 kDa.
IHC-P
Use at an assay dependent concentration.

See Abreview.

ターゲット情報

  • 機能

    As a result of hemolysis, hemoglobin is found to accumulate in the kidney and is secreted in the urine. Haptoglobin captures, and combines with free plasma hemoglobin to allow hepatic recycling of heme iron and to prevent kidney damage. Haptoglobin also acts as an Antimicrobial; Antioxidant, has antibacterial activity and plays a role in modulating many aspects of the acute phase response. Hemoglobin/haptoglobin complexes are rapidely cleared by the macrophage CD163 scavenger receptor expressed on the surface of liver Kupfer cells through an endocytic lysosomal degradation pathway.
    Uncleaved haptoglogin, also known as zonulin, plays a role in intestinal permeability, allowing intercellular tight junction disassembly, and controlling the equilibrium between tolerance and immunity to non-self antigens.

  • 組織特異性

    Expressed by the liver and secreted in plasma.

  • 関連疾患

    Anhaptoglobinemia

  • 配列類似性

    Belongs to the peptidase S1 family.
    Contains 1 peptidase S1 domain.
    Contains 2 Sushi (CCP/SCR) domains.

  • 細胞内局在

    Secreted.
  • Information by UniProt

  • 参照データベース

    • 別名

      • Binding peptide antibody
      • BP antibody
      • Haptoglobin alpha chain antibody
      • Haptoglobin alpha(1S) beta antibody
      • Haptoglobin alpha(2FS) beta antibody
      • Haptoglobin beta chain antibody
      • Haptoglobin, alpha polypeptide antibody
      • Haptoglobin, beta polypeptide antibody
      • HP antibody
      • HP2 ALPHA2 antibody
      • HP2ALPHA2 antibody
      • HPA1S antibody
      • HPT antibody
      • HPT_HUMAN antibody
      • MGC111141 antibody
      • Zonulin antibody
      see all

    参考文献 (17)

    ab13429 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab13429 は 17 報の論文で使用されています。

    • Cao J  et al. Identification of an activation-related protein in B cells in the ABO incompatible condition. Exp Ther Med 19:741-747 (2020). PubMed: 31897108
    • Arya R  et al. Serum Small Extracellular Vesicles Proteome of Tuberculosis Patients Demonstrated Deregulated Immune Response. Proteomics Clin Appl 14:e1900062 (2020). PubMed: 31532894
    • Calvo E  et al. Why Does COVID-19 Affect Patients with Spinal Cord Injury Milder? A Case-Control Study: Results from Two Observational Cohorts. J Pers Med 10:N/A (2020). PubMed: 33096722
    • Gallo JRB  et al. Skin Protein Profile after Major Weight Loss and Its Role in Body Contouring Surgery. Plast Reconstr Surg Glob Open 7:e2339 (2019). PubMed: 31592376
    • Zhu J  et al. Differential Quantitative Determination of Site-Specific Intact N-Glycopeptides in Serum Haptoglobin between Hepatocellular Carcinoma and Cirrhosis Using LC-EThcD-MS/MS. J Proteome Res 18:359-371 (2019). PubMed: 30370771
    View all Publications for this product

    レビューと Q&A

    Show All レビュー Q&A
    レビューを送る 質問を送る

    1-10 of 15 Abreviews or Q&A

    Western blot abreview for Anti-Haptoglobin antibody [HG-36]

     Excellent
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (Liver cell)
    Gel Running Conditions
    Reduced Denaturing
    Loading amount
    20 µg
    Specification
    Liver cell
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
    Read More

    Abcam user community

    Verified customer

    投稿 Sep 29 2021

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Haptoglobin antibody [HG-36]

     Excellent
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (Cancer tissue)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: pH = 8
    Permeabilization
    No
    Specification
    Cancer tissue
    Blocking step
    Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 20°C
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    投稿 Jan 26 2021

    Immunohistochemistry (Frozen sections) abreview for Anti-Haptoglobin antibody [HG-36]

     Good
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Human Tissue sections (Liver)
    Permeabilization
    No
    Specification
    Liver
    Blocking step
    Serum as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 21°C
    Fixative
    Acetone
    Read More

    Abcam user community

    Verified customer

    投稿 Jul 30 2020

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Haptoglobin antibody [HG-36]

     Good
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (Liver cells)
    Permeabilization
    No
    Specification
    Liver cells
    Blocking step
    Serum as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: RT°C
    Fixative
    Acetone
    Read More

    Abcam user community

    Verified customer

    投稿 Jul 23 2020

    Immunocytochemistry abreview for Anti-Haptoglobin antibody [HG-36]

     Excellent
    Abreviews
    abreview image
    Application
    Immunocytochemistry
    Sample
    Human Cultured Cells (Liver cells)
    Permeabilization
    No
    Specification
    Liver cells
    Blocking step
    Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 20°C
    Fixative
    Acetone
    Read More

    Abcam user community

    Verified customer

    投稿 Jun 10 2020

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Haptoglobin antibody [HG-36]

     Excellent
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (Liver)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: EDTA pH = 8
    Permeabilization
    No
    Specification
    Liver
    Blocking step
    Serum as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 30°C
    Fixative
    Paraformaldehyde
    Read More

    HERR DR. Christian Ostalecki

    Verified customer

    投稿 May 25 2018

    Western blot abreview for Anti-Haptoglobin antibody [HG-36]

     Excellent
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Tissue lysate - whole (Lung, lung cancer and liver)
    Loading amount
    30 µg
    Specification
    Lung, lung cancer and liver
    Gel Running Conditions
    Reduced Denaturing (4%stacking, 12%resolving)
    Blocking step
    DIG Wash & Block Buffer, Roche as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 25°C
    Read More

    Abcam user community

    Verified customer

    投稿 Nov 08 2011

    Question

    I think it was a backgrond signal problem. I was used to obtaining a
    high background signal, so when I got such clean slides and I learnt
    about that this product can contain proteasas I logically got
    concerned about the antibody. So I think the antibody is in good
    condition.
    But, anyway, what about this general paragraph?:
    "In most cases, with the possible exception of ascites fluids which
    may contain proteases and should be frozen as soon as possible,
    storage at 4oC upon receipt of the antibody is acceptable for one to
    two weeks, followed by freezing for long-term storage. Again, it is
    important to follow the recommendations on the datasheet."
    There is a contradiction between that paragraph and this one:
    "This antibody can be stored at +4°C short term (1-2 weeks) but we
    would suggest aliquoting and keeping at -20°C or -80°C for longer
    period of time."
    The logic conclusion is that if I keep an antibody coming as ascitic
    fluid and cointaining proteases at +4ºC, as the datasheet says,
    proteases could digest the antibody.
    Regards.

    Read More
    Answer

    Thank you for your email.

    Generally antibodies are stable for 1 year at 4C and for 10 years at -20C or -80C. We however recommend storing the antibodies at 4C for short term (1-2 weeks) because some antibodies lose activity if they kept at 4C for longer period of time. We unfortunately can't run stability test for each >70,000 antibody products available in catalogue. We however do experiments for checking the stability with a range of antibodies every year. The data is available on request. Based on the data we have, we choose the statement which is best suitable for all of the products.

    Finally, I suggest not to worry about the stability; the product is guaranteed for 6 months if stored as recommended on the datasheet.

    I hope this info will be helpful.

    Read More

    Question

    Thank you for your answer.
    I have read the information shown at this site:
    https://www.abcam.com/index.html?pageconfig=resource&rid=10795
    and I have a critical question after reading this paragraph:
    "In most cases, with the possible exception of ascites fluids which
    may contain proteases and should be frozen as soon as possible,
    storage at 4oC upon receipt of the antibody is acceptable for one to
    two weeks, followed by freezing for long-term storage. Again, it is
    important to follow the recommendations on the datasheet."
    The Anti-Haptoglobin antibody [HG-36] (ab13429) comes as ascitic
    fluid. However, in its datasheet nothing is said about the possibility
    of containing proteases, so that it should be frozen as soon as
    possible. As I didn't know about this problem I kept it for
    approximately 26 hours at 4ºC. Moreover, the antibody was shipped
    refrigerated, so I don't really know how long it was not frozen.
    After running a immunohistochemical test using this antiboy and
    observing an unexpected result I'm seriously thinking about the
    possibility the antibody could be damaged.
    Could you put some light on this matter?
    Regards

    Read More
    Answer

    Thank you for your response and your comment.
    The on-line document contains general information/guidelines for our antibodies for different purifications. Each product has specific storage instructions which can be found on the public website (on-line product datasheet).
    This antibody can be stored at +4°C short term (1-2 weeks) but we would suggest aliquoting and keeping at -20°C or -80°C for longer period of time.
    If you are happy to share some further details of the IHC protocol; I could certainly take a look at it. There may be some tips/optimization I could perhaps offer if it is necessary and appropriate.
    It would be appreciated if you could complete the following form (attached as a word document) and attach an image to the response.
    I look forward to hearing from you soon and resolving this issue as soon as possible.

    Read More

    Question

    I recently bought Anti-Haptoglobin antibody [HG-36] (ab13429) from
    your company and I wonder how to make dilutions of the antibody.
    Taking into account that this product comes with sodium azide as a
    preservative, my question is: is PBS a proper diluter?
    Regards

    Read More
    Answer

    Thank you for your enquiry and your interest in our products.
    This antibody is ascitic fluid and the storage buffer contains 15mM sodium azide. You could use PBS for diluting the antibody. We have some tips/recommendations at this site, you may find it useful.
    https://www.abcam.com/index.html?pageconfig=resource&rid=10795
    https://www.abcam.com/ps/pdf/protocols/antibody_storage.pdf
    I hope this helps and if I can assist further, please do not hesitate to contact me.

    Read More

    1-10 of 15 Abreviews or Q&A

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