HA tag peptide (ab13835)

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Western blot - HA tag peptide (ab13835)

    Key features and details

    • Suitable for: Blocking

    製品の詳細

    • 製品名

      HA tag peptide

    • Animal free

      No

    • 由来

      Synthetic

      関連製品

      特性

      Our Abpromise guarantee covers the use of ab13835 in the following tested applications.

      The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

      • アプリケーション

        Blocking - Blocking peptide for Anti-HA tag antibody (ab13834)

      • 製品の状態

        Liquid

      • 備考

        - First try to dissolve a small amount of peptide in either water or buffer. The more charged residues on a peptide, the more soluble it is in aqueous solutions.
        - If the peptide doesn’t dissolve try an organic solvent e.g. DMSO, then dilute using water or buffer.
        - Consider that any solvent used must be compatible with your assay. If a peptide does not dissolve and you need to recover it, lyophilise to remove the solvent.
        - Gentle warming and sonication can effectively aid peptide solubilisation. If the solution is cloudy or has gelled the peptide may be in suspension rather than solubilised.
        - Peptides containing cysteine are easily oxidised, so should be prepared in solution just prior to use.

      • Concentration information loading...

      前処理および保存

      • 保存方法および安定性

        Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

        Information available upon request.

      関連情報

      • 別名

        • HA epitope tag
        • HA1
        • HA2
        • hemagglutinin
        • Hemagglutinin HA1 chain
        • Hemagglutinin HA2 chain
        see all

      • 関連性

        Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins.

      画像

      • Western blot - HA tag peptide (ab13835)
        Western blot - HA tag peptide (ab13835)
        Western blot using ab13834 on 293 cells transfected with a 55 kDa HA-tagged minigene (ab20896). Lane 1: ab13834 Lane 2: ab13834 with blocking peptide ab13835. Primary: Rabbit polyclonal to HA tag (1/500) Secondary: Alexa Fluor 680 Goat anti Rabbit IgG (1/5000). Lysates at 10µg/lane.

      プロトコール

      To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

      Click here to view the general protocols

      参考文献 (2)

      ab13835 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

      ab13835 は 2 報の論文で使用されています。

      • Zhao Y  et al. Arabidopsis Duodecuple Mutant of PYL ABA Receptors Reveals PYL Repression of ABA-Independent SnRK2 Activity. Cell Rep 23:3340-3351.e5 (2018). PubMed: 29898403
      • Zhao Y  et al. ABA receptor PYL9 promotes drought resistance and leaf senescence. Proc Natl Acad Sci U S A 113:1949-54 (2016). PubMed: 26831097

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      Question


      I am attempting to find a product that would be used to create a standard curve for a quantitative western. My protein of interest has an HA-tag but cannot really be purified, would product ab13835 be suitable for creating a standard curve in a western?

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      Answer

      Thank you for contacting Abcam regarding ab13835.


      This product is the HA peptide used as the immunogen to generate the antibody ab13834. Since it is only 9aa, it cannot be run on a gel to use as a positive control or standard. Instead, I would recommend a purified HA-tagged protein or a cell lysate containing an HA tagged protein, such as ab20896.


      I hope this information is helpful. Please do not hesitate to contact me if you have any additional questions.

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      Question

      Dear Tech Support Team,

      Our customer wishes to use ab13835 in IP with anti HA from Santa Cruz SC-805. Kindly advise if ab13835 is suitable for this purpose. Will the mentioned above antibody recognize ab13835?

      Thanks in advance for your assistance and reply.

      Kind Regards,

      Read More
      Answer

      Thank you for contacting us.
      Yes, we expect that antibody to bind this peptide. The peptide ab13835 does contain the classical HA tag (amino acid 98 to 106 of the surface hemagglutinin glycoprotein of Human influenza virus). The cited antibody is directed against the HA tag and should therefore be able to recongize this peptide ab13835.
      I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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      Question

      Thank you very much for your important information. I'm wondering do you have some experience of using this HA peptide (ab13835) for elution, such as the quantity needed for elution, etc? I did not find such information on the data sheet.

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      Answer

      Here is a general protocol for eluting your HA-tagged protein with ab13835, our HA tag peptide: The peptide arrives at a 1mg/ml concentration. After the last wash, remove all of the wash buffer and add a sufficient volume of the peptide for your downstream use. For example, 30-50ul of the peptide might be a useful volume. Elute for at least 1 hour at 4 degrees C with gentle agitation. Pellet the beads and remove the supernatant. Confirm elution of target protein via coomassie staining and/or western blotting.

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      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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