Application
Western blot
Sample
Human Cell lysate - whole cell (HEK293)
Gel Running Conditions
Reduced Denaturing (4-20)
Loading amount
10 µg
Treatment
transfected with HA-tagged protein
Specification
HEK293
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C
Dilution
1/2000
Secondary antibody
Dilution
1/5000
Name
Non-Abcam antibody was used: santa cruz anti-mouse
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL prime
Negative control
cell transfected with empty vector (lane 1)
Exposure
1 second(s)
Bands
Specific: 70 kDa Non-specific: 150 kDa
Positive control
cell transfected with HA-tag protein (lane 2, 3)
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投稿 Jul 02 2021