Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (frozen liver sections)
Permeabilization
Yes - Triton X 0.3% - 10 min
Specification
frozen liver sections
Fixative
Paraformaldehyde
Other product details
Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: PBS
Dilution
1/400
Secondary antibody
Dilution
1/400
Name
Non-Abcam antibody was used: Alexa Fluor 488 goat anti-mouse
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Additional data
Additional Notes
The antibody induced unspecific cytoplasmic signals in both positive and negative control
Abcam response
Thank you for submitting an AbReview for ab18181 (Anti-HA tag antibody [HA.C5]). It is unfortunate that the outcome of your experiment using ab18181 is inconclusive. This antibody has not been tested in IHC-Fr before and it was not known if ab18181 is suitable for this application. However, further optimization of the protocol may improve the results obtained.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
投稿 Jun 30 2016