Application
Western blot
Loading amount
50 µg
Gel Running Conditions
Reduced Denaturing (15%)
Sample
Human Cell lysate - whole cell (Renal cancer cell line)
Specification
Renal cancer cell line
Treatment
Doxicycline induction of Protein_HA tag
Blocking step
Li-cor Blocking Buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Other product details
Incubation time
2 hour(s) and 0 minute(s) · Temperature: 25°C
Dilution
1/1000
Secondary antibody
Name
Abcam antibody:Donkey Anti-Rabbit IgG H&L (Alexa Fluor® 680) preadsorbed
Dilution
1/5000
Detection
Detection method
LICOR
Bands
Specific: 25 kDa
Exposure
30 second(s)
Negative control
Non treated cells. Line 1
Positive control
Doxicycline treated cells. Line 2
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Jul 28 2014