Anti-HA tag 抗体 [HA.C5] (ab18181)
![Western blot - Anti-HA tag antibody [HA.C5] (ab18181)](https://www.abcam.co.jp/ps/products/18/ab18181/Images/ab18181-212146-anti-ha-tag-antibody-hac5-western-blot.jpg "Western blot - Anti-HA tag antibody [HA.C5] (ab18181)")
Key features and details
- Mouse monoclonal [HA.C5] to HA tag
- Suitable for: WB, ICC/IF
- Reacts with: Species independent
- Isotype: IgG3
製品の概要
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製品名
Anti-HA tag antibody [HA.C5]
HA tag 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [HA.C5] to HA tag -
由来種
Mouse -
アプリケーション
適用あり: WB, ICC/IFmore details -
種交差性
交差種: Species independent -
免疫原
Synthetic peptide from influenza hemagglutinin epitope:
YPYDVPDYA
conjugated to KLH. -
特記事項
This product was changed from ascites to tissue culture supernatant on 5th February 2018. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
バッファー
pH: 7.20
Preservative: 0.05% Sodium azide
Constituent: PBS -
Concentration information loading... -
精製度
Affinity purified -
特記事項（精製）
Purified from TCS -
ポリ/モノ
モノクローナル -
クローン名
HA.C5 -
アイソタイプ
IgG3 -
研究分野
関連製品
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ChIP Related Products
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Compatible Secondaries
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Isotype control
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Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab18181の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | (12) |
1/1000.
|
| ICC/IF | (4) |
Use at an assay dependent concentration.
|
| 特記事項 |
|---|
|
WB
1/1000. |
|
ICC/IF
Use at an assay dependent concentration. |
ターゲット情報
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関連性
Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins. -
別名
- HA epitope tag antibody
- HA1 antibody
- HA2 antibody
see all
画像
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Western blot - Anti-HA tag antibody [HA.C5] (ab18181)This image is courtesy of an anonymous AbreviewAll lanes : Anti-HA tag antibody [HA.C5] (ab18181) at 1/1000 dilution
Lanes 1-2 : HEK293 whole cell lysate - transfected
Lane 3 : HEK293 whole cell lysate - untransfected
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : IRDye® 800CW Goat anti-mouse IgG polyclonal at 1/10000 dilution
Performed under reducing conditions.
Observed band size: 85 kDa why is the actual band size different from the predicted?
Exposure time: 5 minutes
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![Immunocytochemistry/ Immunofluorescence - Anti-HA tag antibody [HA.C5] (ab18181)](https://www.abcam.co.jp/ps/products/18/ab18181/Images/ab18181-26826-anti-ha-tag-antibody-hac5-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-HA tag antibody [HA.C5] (ab18181)This image is courtesy of an Abreview submitted by Harendra Chaharab18181 staining HA tag (green) in HeLa cells by Immunocytochemistry/ Immunofluorescence.
Cells were fixed with paraformaldehyde, permeabilized with 0.5% Triton X-100 and blocked with 3% BSA for 1 hour at 22°C. Samples were incubated with primary antibody (1/1000 in diluent) for 1 hour at 22°C. A FITC-conjugated goat anti-mouse polyclonal IgG (1/1000) was used as the secondary antibody. Nuclei were stained with DAPI (blue). -
![Western blot - Anti-HA tag antibody [HA.C5] (ab18181)](https://www.abcam.co.jp/ps/products/18/ab18181/Images/ab18181-26829-anti-ha-tag-antibody-hac5-western-blot.jpg)
Western blot - Anti-HA tag antibody [HA.C5] (ab18181)Western blot using ab18181 of 293 cells transfected with HA-tagged vector(2) and untransfected control (1). Western blot using ab18181 of 293 cells transfected with HA-tagged vector(2) and untransfected control (1). -
![Immunocytochemistry/ Immunofluorescence - Anti-HA tag antibody [HA.C5] (ab18181)](https://www.abcam.co.jp/ps/products/18/ab18181/Images/ab18181-26828-anti-ha-tag-antibody-hac5-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-HA tag antibody [HA.C5] (ab18181)Immunofluorescence using ab18181 staining a HA-tag fusion protein (transcription factor) in a stable expressing cell line (right hand panel) and control cell line (left hand panel). -
![Western blot - Anti-HA tag antibody [HA.C5] (ab18181)](https://www.abcam.co.jp/ps/products/18/ab18181/Images/ab18181-26827-anti-ha-tag-antibody-hac5-western-blot.jpg)
Western blot - Anti-HA tag antibody [HA.C5] (ab18181)This image is courtesy of an anonymous AbreviewAll lanes : Anti-HA tag antibody [HA.C5] (ab18181) at 1/2000 dilution
Lane 1 : WCE from cell line transfected for HA-tagged protein
Lane 2 : WCE from a cell line transfected with empty vector
Lysates/proteins at 50 µg per lane.
Secondary
All lanes : HRP conjugated Goat anti-mouse IgG (H+L)
Developed using the ECL technique.
Performed under reducing conditions.
Exposure time: 10 seconds
Incubation with the primary antibody was carried out at 4°C overnight, whilst the secondary antibody was incubated for 1 hour at room temperature.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (192)
ab18181 は 192 報の論文で使用されています。
- Dong H et al. ELONGATED HYPOCOTYL 5 mediates blue light-induced starch degradation in tomato. J Exp Bot 72:2627-2641 (2021). PubMed: 33377142
- Shen M et al. Structural basis for the multi-activity factor Rad5 in replication stress tolerance. Nat Commun 12:321 (2021). PubMed: 33436623
- Malik AU et al. Deciphering the LRRK code: LRRK1 and LRRK2 phosphorylate distinct Rab proteins and are regulated by diverse mechanisms. Biochem J 478:553-578 (2021). PubMed: 33459343
- Eastwood EL et al. Dimerisation of the PICTS complex via LC8/Cut-up drives co-transcriptional transposon silencing in Drosophila. Elife 10:N/A (2021). PubMed: 33538693
- Meng J et al. PFKFB4 promotes lung adenocarcinoma progression via phosphorylating and activating transcriptional coactivator SRC-2. BMC Pulm Med 21:60 (2021). PubMed: 33593309