Anti-HA tag 抗体 [EPR22819-101] (ab236632)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR22819-101] to HA tag
- Suitable for: Flow Cyt (Intra), WB, IP, ICC/IF
- Reacts with: Species independent
Related conjugates and formulations
製品の概要
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製品名
Anti-HA tag antibody [EPR22819-101]
HA tag 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR22819-101] to HA tag -
由来種
Rabbit -
アプリケーション
適用あり: Flow Cyt (Intra), WB, IP, ICC/IFmore details
適用なし: ChIP -
種交差性
交差種: Species independent -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ・コントロール
- WB: HEK-293T and HEK-293T transfected with TIM 4 lysates. ICC/IF: HEK-293T cells transfected with HA-tagged TATA-box-binding protein (WT) expression vector.Flow Cyt (intra): HEK-293T transfected with TATA-box-binding protein (WT) expression vector containing HA-tag cells. IP: HEK-293T transfected with TIM 4 (WT) expression vector containing a HA-GFP-tag and PDGFR TM domain whole cell lysate.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR22819-101 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab236632の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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Flow Cyt (Intra) |
1/600.
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WB |
1/1000.
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IP |
1/30.
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ICC/IF |
1/1000.
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特記事項 |
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Flow Cyt (Intra)
1/600. |
WB
1/1000. |
IP
1/30. |
ICC/IF
1/1000. |
ターゲット情報
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関連性
Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins. -
別名
- HA epitope tag antibody
- HA1 antibody
- HA2 antibody
see all
画像
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All lanes : Anti-HA tag antibody [EPR22819-101] (ab236632) at 1/1000 dilution
Lane 1 : HEK-293T (human embryonic kidney) transfected with an empty vector (vector control), containing a HA-myc-His-GFP-tag and PDGFR TM domain, whole cell lysate
Lane 2 : HEK-293T transfected with TIM 4 (WT) expression vector containing a HA-myc-His-GFP-tag and PDGFR TM domain, whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Observed band size: 37,78 kDa why is the actual band size different from the predicted?Antibodies used in the control panels are (Anti-TIM 4 antibody), ab9110 (Anti-HA tag antibody - ChIP Grade) and ab181602 (Anti-GAPDH antibody [EPR16891] - Loading Control).
Exposure time: 3 minutes.
Blocking and Diluting Buffer and concentration: 5% NFDM/TBST.
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HA tag was immunoprecipitated from 0.35 mg HEK-293T (human embryonic kidney) transfected with TIM 4 (WT) expression vector containing a HA-GFP-tag and PDGFR TM domain whole cell lysate 10µg with ab236632 at 1/30 dilution (2µg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab236632. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1/2000 dilution.
Lane 1: HEK-293T (human embryonic kidney) transfected with TIM 4 (WT) expression vector containing a HA-GFP-tag and PDGFR TM domain whole cell lysate 10µg.
Lane 2: ab236632 IP in HEK-293T transfected with TIM 4 (WT) expression vector containing a HA-GFP-tag and PDGFR TM domain whole cell lysate.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab236632 in HEK-293T transfected with TIM 4 (WT) expression vector containing a HA-GFP-tag and PDGFR TM domain whole cell lysate.
Blocking and dilution buffer and concentration/ 5% NFDM/TBST.
Exposure time: 3 seconds.
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Intracellular flow cytometric analysis of 4% paraformaldehyde fixed, 90% methanol permeabilized HEK-293T (human embryonic kidney) transfected with TATA-box-binding protein (WT) expression vector containing HA-tag (Right) / HEK-293T (Left) cells labeling HA tag with ab236632 at 1/600 dilution. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.
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Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HEK-293T (human embryonic kidney epithelial cell) transfected with HA-tagged TATA-box-binding protein (WT) expression vector labeling HA tag with ab236632 at 1/1000 (0.6 µg/ml) dilution, followed by ab150077 AlexaFluor®488 Goat anti-Rabbit secondary antibody at 1/1000, (2 µg/ml) dilution (Green). Confocal image showing nuclear staining in HEK-293T cells transfected with HA-tagged TATA-box-binding protein (WT) expression vector is observed. Anti-HA.11 Epitope Tag antibody (Alexa Fluor® 647) was used to counterstain tubulin at 1/200 dilution (Red). The nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 (2 µg/ml) dilution.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
Certificate of Compliance
参考文献 (13)
ab236632 は 13 報の論文で使用されています。
- Chen S et al. The Role of REC8 in the Innate Immune Response to Viral Infection. J Virol 96:e0217521 (2022). PubMed: 35107381
- Wang Z et al. Oleanolic Acid Derivative AXX-18 Exerts Antiviral Activity by Inhibiting the Expression of HSV-1 Viral Genes UL8 and UL52. Viruses 14:N/A (2022). PubMed: 35746758
- Lu C et al. Phosphorylation of PFKFB4 by PIM2 promotes anaerobic glycolysis and cell proliferation in endometriosis. Cell Death Dis 13:790 (2022). PubMed: 36109523
- Fei X et al. TRIM22 orchestrates the proliferation of GBMs and the benefits of TMZ by coordinating the modification and degradation of RIG-I. Mol Ther Oncolytics 26:413-428 (2022). PubMed: 36159777
- Wei G et al. Prion-like low complexity regions enable avid virus-host interactions during HIV-1 infection. Nat Commun 13:5879 (2022). PubMed: 36202818