Application
ChIP
Detection step
Real-time PCR
Sample
Mouse Cell lysate - whole cell (Embryonic Stem Cells)
Specification
Embryonic Stem Cells
Negative control
The parent cell line.
Type
Cross-linking (X-ChIP)
Duration of cross-linking step: 0 second(s)
Specification of the cross-linking agent: Disuccinimidyl glutarate
Duration of cross-linking step: 0 second(s)
Specification of the cross-linking agent: Disuccinimidyl glutarate
Positive control
A cell line, which stably express an HA-tagged RARgamma protein.
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: RIPA
Dilution
1/200
Additional data
PubMed ID
20857416: View
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Mr. Dan Stummer
Verified customer
投稿 Mar 28 2014