The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/50 - 1/100. Detects a band of approximately 50 kDa.
Use at an assay dependent concentration.
1/10 - 1/50.
Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins.
HA epitope tag antibody
Hemagglutinin HA1 chain antibody
Hemagglutinin HA2 chain antibody
Western blot - Anti-HA tag antibody (ab71113)
All lanes : Anti-HA tag antibody (ab71113)
All lanes : HA-tagged recombinant protein bacterial lysate
Immunocytochemistry/ Immunofluorescence - Anti-HA tag antibody (ab71113)
Immunofluorescent staining of MRC-5 fibroblasts transiently transfected with either wild-type (wt) or a GTPase-deficient dynamin-2 mutant (K44A) with a hemagglutinin (HA) tag using ab71113.
ChIP - Anti-HA tag antibody (ab71113)
ChIP was performed with 35S:HATCL1 plants using anti-HA antibodies. Rabbit preimmune serum was used as a mock control. Primer sets specific for the first intron or the 3-UTR region of GL1 were used in PCR reactions. ACTIN2 was used as a control.