Anti-HA tag 抗体 (ab13834)
Key features and details
- Rabbit polyclonal to HA tag
- Suitable for: WB
- Reacts with: Species independent
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-HA tag antibody
HA tag 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to HA tag -
由来種
Rabbit -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Species independent -
免疫原
Synthetic peptide within HA tag conjugated to keyhole limpet haemocyanin. The exact sequence is proprietary.
(Peptide available asab13835) -
ポジティブ･コントロール
- WB: HEK-293 (Human) Whole Cell Lysate - expressing HA tag protein.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Compatible Secondaries
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Immunizing Peptide (Blocking)
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab13834の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | (2) |
1/500. Can be blocked with HA tag peptide (ab13835).
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| 特記事項 |
|---|
|
WB
1/500. Can be blocked with HA tag peptide (ab13835). |
ターゲット情報
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関連性
Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins. -
別名
- HA epitope tag antibody
- HA1 antibody
- HA2 antibody
see all
画像
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Western blot - Anti-HA tag antibody (ab13834)All lanes : Anti-HA tag antibody (ab13834) at 1/500 dilution
Lane 1 : HEK-293 (Human) Whole Cell Lysate - expressing HA tag protein (ab20896)
Lane 2 : HEK-293 (Human) Whole Cell Lysate - expressing HA tag protein (ab20896) withHA tag peptide (ab13835) at 1 µg/ml
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Alexa Fluor 680 Goat-anti-Rabbit IgG at 1/5000 dilution
Performed under reducing conditions.
データシートおよび資料
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Datasheet download
参考文献 (7)
ab13834 は 7 報の論文で使用されています。
- Wang C et al. Heat shock protein DNAJA1 stabilizes PIWI proteins to support regeneration and homeostasis of planarian Schmidtea mediterranea. J Biol Chem 294:9873-9887 (2019). PubMed: 31076507
- Chen X et al. Deficiency or inhibition of lysophosphatidic acid receptor 1 protects against hyperoxia-induced lung injury in neonatal rats. Acta Physiol (Oxf) 216:358-75 (2016). PubMed: 26495902
- Myers SA et al. SOX2 O-GlcNAcylation alters its protein-protein interactions and genomic occupancy to modulate gene expression in pluripotent cells. Elife 5:e10647 (2016). PubMed: 26949256
- Huang Y et al. UXT-V1 protects cells against TNF-induced apoptosis through modulating complex II formation. Mol Biol Cell 22:1389-97 (2011). WB ; Human . PubMed: 21307340
- Alhamidi M et al. Fukutin-Related Protein Resides in the Golgi Cisternae of Skeletal Muscle Fibres and Forms Disulfide-Linked Homodimers via an N-Terminal Interaction. PLoS One 6:e22968 (2011). WB . PubMed: 21886772
- Reszka N et al. Simian TRIM5alpha proteins reduce replication of herpes simplex virus. Virology 398:243-50 (2010). PubMed: 20060996
- Ichimura K et al. IDH1 mutations are present in the majority of common adult gliomas but rare in primary glioblastomas. Neuro Oncol 11:341-7 (2009). WB . PubMed: 19435942
