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  1. Link

    ha-tag-antibody-4c12-ab1424.pdf

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Tags & Cell Markers Epitope Tags HA Tag
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Anti-HA tag 抗体 [4C12] (ab1424)

  • Datasheet
  • SDS
Reviews (12)Q&A (13)References (37)

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出荷および受注について

Abpromise

保証された製品品質、優れたカスタマー・サポート。 詳細はこちら。

Western blot - Anti-HA tag antibody [4C12] (ab1424)
  • Immunocytochemistry/ Immunofluorescence - Anti-HA tag antibody [4C12] (ab1424)
  • Immunoprecipitation - Anti-HA tag antibody [4C12] (ab1424)
  • ChIP - Anti-HA tag antibody [4C12] (ab1424)

Key features and details

  • Mouse monoclonal [4C12] to HA tag
  • Suitable for: ICC/IF, ChIP, IP, WB
  • Reacts with: Species independent
  • Isotype: IgG1

こちらの製品もご検討ください

一次抗体
Product image
HRP Anti-HA tag antibody (ab183884)
二次抗体
Product image
Goat Anti-Mouse IgG H&L (HRP) (ab205719)

関連製品

医薬用外毒物

製品の概要

  • 製品名

    Anti-HA tag antibody [4C12]
    HA tag 一次抗体 製品一覧
  • 製品の詳細

    Mouse monoclonal [4C12] to HA tag
  • 由来種

    Mouse
  • 特異性

    The antibody detects HA-tag fused to the C-terminus or N-terminus of targeted proteins in transfected cells. The antibody works in ChIP but less well than ab9110 (see ChIP data).
  • アプリケーション

    適用あり: ICC/IF, ChIP, IP, WBmore details
  • 種交差性

    交差種: Species independent
  • ポジティブ・コントロール

    • WB: Rab6-HA transfected HEK-293T whole cell lysate. IP: Rab6-HA transfected HEK-293T whole cell lysate. ICC/IF: Baby hamster kidney cell expressing HA-tag CFTR protein. ChIP: Xenopus laevis oocytes injected with mRNA for HA-tagged human BORIS.
  • 特記事項

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

製品の特性

  • 製品の状態

    Liquid
  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファー

    Preservative: 0.02% Thimerosal (merthiolate)
    Constituents: 50% Glycerol (glycerin, glycerine), 1% BSA, PBS
  • Concentration information loading...
  • ポリ/モノ

    モノクローナル
  • クローン名

    4C12
  • アイソタイプ

    IgG1
  • 研究分野

    • Tags & Cell Markers
    • Epitope Tags
    • HA Tag

関連製品

  • ChIP Related Products

    • Rabbit Anti-Mouse IgG H&L (ab46540)
    • ChIP Kit (ab500)
  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
  • Isotype control

    • Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)
  • Related Products

    • HA tag peptide (ab13835)

アプリケーション

Our Abpromise guarantee covers the use of ab1424 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use a concentration of 10 µg/ml.
ChIP Use a concentration of 3 µg/ml. Use 3 µg per 25 µg chromatin (see figure legend).
IP Use a concentration of 10 µg/ml.
WB Use a concentration of 1 µg/ml.

ターゲット情報

  • 関連性

    Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins.
  • 別名

    • HA epitope tag antibody
    • HA1 antibody
    • HA2 antibody
    • hemagglutinin antibody
    • Hemagglutinin HA1 chain antibody
    • Hemagglutinin HA2 chain antibody
    see all

画像

  • Western blot - Anti-HA tag antibody [4C12] (ab1424)
    Western blot - Anti-HA tag antibody [4C12] (ab1424)This image is courtesy of an anonymous Abreview
    Anti-HA tag antibody [4C12] (ab1424) at 1/1000 dilution + HEK293T whole cell lysate over-expressing HA-tagged Rab6 at 20 µg

    Secondary
    HRP-conjugated goat anti-mouse polyclonal IgG at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Exposure time: 5 seconds


    Blocked with 5% milk for 30 min at 25°C

    See Abreview

  • Immunocytochemistry/ Immunofluorescence - Anti-HA tag antibody [4C12] (ab1424)
    Immunocytochemistry/ Immunofluorescence - Anti-HA tag antibody [4C12] (ab1424)This image was kindly supplied as part of the review submitted by one of our customers.

    This picture shows baby hamster kidney cell expressing HA-tag CFTR protein. This type of cells were grown on coverslip and immunofluorescence was performed. Dilution of 1:200 was used and incubated for 2hrs with antibody.

  • Immunoprecipitation - Anti-HA tag antibody [4C12] (ab1424)
    Immunoprecipitation - Anti-HA tag antibody [4C12] (ab1424)Data courtesy of an anonymous AbReview

    Recombinant HA-tagged Rab6 was immunoprecipitated from 1 mg transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract with 2 μL ab1424. 

    Lane 1: Rab6-HA transfected HEK-293T whole cell extract (Input)

    Lane 2: ab1424 IP in Rab6-HA transfected HEK-293T whole cell extract.

     

  • ChIP - Anti-HA tag antibody [4C12] (ab1424)
    ChIP - Anti-HA tag antibody [4C12] (ab1424)

    Xenopus laevis oocytes were injected with mRNA for HA-tagged human BORIS. Chromatin was prepared according to the Abcam X-ChIP protocol. Oocytes were fixed with formaldehyde for 10min. The  ChIP was performed with 25µg of chromatin, 3µg of ab1424 (anti-HA, light blue)  and 3µg of ab18337 (anti-BORIS, dark blue), and 20µl of Protein A/G sepharose beads. A non-specific antibody was used as a control (yellow). The immunoprecipitated DNA was quantified by real time PCR (Taqman approach).

プロトコール

  • ChIP protocols
  • Immunoprecipitation protocols
  • Immunocytochemistry & immunofluorescence protocols
  • Western blot protocols

Click here to view the general protocols

データシートおよび資料

    • Datasheet
    • SDS
  • 参考文献 (37)

    ab1424 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab1424 は 37 報の論文で使用されています。

    • Wang L  et al. Nuclear hnRNPA2B1 initiates and amplifies the innate immune response to DNA viruses. Science 365:N/A (2019). PubMed: 31320558
    • Wang Y  et al. p300 Acetylates JHDM1A to inhibit osteosarcoma carcinogenesis. Artif Cells Nanomed Biotechnol 47:2891-2899 (2019). PubMed: 31307234
    • Wang H  et al. Deletion of OSBPL2 in auditory cells increases cholesterol biosynthesis and drives reactive oxygen species production by inhibiting AMPK activity. Cell Death Dis 10:627 (2019). PubMed: 31427568
    • Xiang T  et al. Tumor suppressive BTB/POZ zinc-finger protein ZBTB28 inhibits oncogenic BCL6/ZBTB27 signaling to maintain p53 transcription in multiple carcinogenesis. Theranostics 9:8182-8195 (2019). PubMed: 31754389
    • James Theoga Raj C  et al. The copper-sensing transcription factor Mac1, the histone deacetylase Hst1, and nicotinic acid regulate de novo NAD+ biosynthesis in budding yeast. J Biol Chem 294:5562-5575 (2019). PubMed: 30760525
    View all Publications for this product

    レビューと Q&A

    Show All レビュー Q&A
    レビューを送る 質問を送る

    1-10 of 25 Abreviews or Q&A

    Immunoprecipitation abreview for Anti-HA tag antibody [4C12]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunoprecipitation
    Sample
    Human Cell lysate - whole cell (Human osteosarcoma)
    Total protein in input
    1000 µg
    Treatment
    Plasmid transfection for 24 hrs
    Immuno-precipitation step
    Protein A/G
    Specification
    Human osteosarcoma
    Read More

    Miss. Can Zhou

    Verified customer

    投稿 Jan 28 2016

    Western blot abreview for Anti-HA tag antibody [4C12]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Cell lysate - whole cell (Myoblast)
    Gel Running Conditions
    Non-reduced Denaturing (10% gel)
    Loading amount
    40 µg
    Treatment
    Plasmid transfection
    Specification
    Myoblast
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 18°C
    Read More

    Abcam user community

    Verified customer

    投稿 Jan 28 2016

    Immunocytochemistry/ Immunofluorescence abreview for Anti-HA tag antibody [4C12]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (vascular smooth muscle cell)
    Permeabilization
    Yes - NP40
    Specification
    vascular smooth muscle cell
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 21°C
    Fixative
    Formaldehyde
    Read More

    Abcam user community

    Verified customer

    投稿 Jan 06 2016

    Western blot abreview for Anti-HA tag antibody [4C12]

    Average
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Loading amount
    20 µg
    Gel Running Conditions
    Reduced Denaturing (10)
    Sample
    Human Cell lysate - whole cell (HEK293)
    Specification
    HEK293
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
    Read More

    Abcam user community

    Verified customer

    投稿 Mar 03 2015

    Immunoprecipitation abreview for Anti-HA tag antibody [4C12] - ChIP Grade

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunoprecipitation
    Sample
    Human Cell lysate - whole cell (Retro virus transduction for protein X)
    Total protein in input
    500 µg
    Specification
    Retro virus transduction for protein X
    Immuno-precipitation step
    Protein A/G
    Read More

    Abcam user community

    Verified customer

    投稿 Feb 26 2013

    Immunoprecipitation abreview for Anti-HA tag antibody [4C12] - ChIP Grade

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunoprecipitation
    Sample
    Human Cell lysate - whole cell (HEK 293T cells)
    Total protein in input
    1000 µg
    Specification
    HEK 293T cells
    Immuno-precipitation step
    Protein A
    Read More

    Abcam user community

    Verified customer

    投稿 Jan 16 2013

    Western blot abreview for Anti-HA tag antibody [4C12] - ChIP Grade

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (HEK293T)
    Loading amount
    20 µg
    Specification
    HEK293T
    Gel Running Conditions
    Reduced Denaturing (12% Tris-glycine gel)
    Blocking step
    Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More

    Abcam user community

    Verified customer

    投稿 Sep 17 2012

    Question

    Thanks very much for your quick reply. I'm interested in the testing discount program you mentioned. Could you please let me know more about it?

    Read More

    Abcam community

    Verified customer

    Asked on Aug 07 2012

    Answer

    Thank you for your reply.

    You can find out more about the 100% Abreview Testing Discount Program below:

    https://www.abcam.com/index.html?pageconfig=resource&rid=11998&viapagetrap=collaborationdiscount



    For all the antibodies that you are interested in, we already guarantee them to work in ChIP experiments, regardless of species. Therefore, they would not qualify for the testing discount program, but they are covered under our Abpromise.

    If there is anything else I can help you with, or if you are interested in any of our other antibodies that have not been tested in ChIP, but you might be interested in them, then please let me know.

    Read More

    Abcam Scientific Support

    Answered on Aug 07 2012

    Question

    Dear Sir/Madam, I'm doing a ChIP experiment using drosophila tissue. Your ChIP-grade anti-epitope tag antibodies may be very helpful for me. The Abcam antibodies usually work well for us, so I would like to give it a try. But before we place a order, I'm wondering whether I can get a small aliquot of the antibodies to see whether they would work for my tissue. I'm particularly interested in AbID 9110 (or 1424) and 9132. Please let me know. hank you very much in advance!

    Read More

    Abcam community

    Verified customer

    Asked on Aug 06 2012

    Answer

    Thank you for contacting Abcam. Because we carry over 90,000 products, it isn't feasible for us to keep small sample sizes of our products.

    We are happy to reassure our customers that all of our products are covered by our Abpromise, which guarantees that the product will work in the applications and species specified on the datasheet, or we will offer a replacement, credit, or refund within 6 months of purchase.

    If the product is to be used in an untested species or application, you may be eligible for our testing discount program if the antibody has not yet been purchased. Please contact our Scientific Support team by replying to this email prior to purchase for more information.

    Otherwise, we like to encourage all of our customers to submit an Abreview via the online product datasheet. We always appreciate customer feedback, whether positive or negative, and we make all product information available to researchers. Plus, each Abreview earns Abpoints that can be used for discounts on future purchases or rewards such as Amazon.com gift certificates.

    To find out more about our Abreview system, please see the following link:

    https://www.abcam.com/abreviews

    I hope this information is helpful. Please do not hesitate to contact us again with any other questions.

    Read More

    Abcam Scientific Support

    Answered on Aug 06 2012

    Question

    Our lab is trying to use these antibodies (ab9110 and ab1424) to do immunohistochemistry on sections from various mouse organs. We were wondering if these antibodies are tested and approved for use on paraffin sections only, cryopreserved sections only, or both?

    Read More

    Abcam community

    Verified customer

    Asked on Sep 06 2011

    Answer

    Thank you for contacting us. Both antibodies have not yet been tested in any IHC application (neither IHC-P or IHC-Fr). We do have a testing discount program that you can use in that case. Here is some information about how the testing discount program works: The testing discount program is for customers who like to use an antibody/protein on an untested species/application. You would purchase the antibody at full price, test it and submit an Abreview with your data (positive or negative). On your next order you will receive a discount for ONE antibody at the full price (100%) of the antibody you have tested. The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount. I'd be happy to issue you a discount code for one or both antibodies. Please let me know. Also, please do not hesitate to contact us if you need any more advice or information.

    Read More

    Abcam Scientific Support

    Answered on Sep 06 2011

    1-10 of 25 Abreviews or Q&A

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