Anti-HA tag 抗体 [4C12] (ab1424)

Reviews (12)Q&A (13)References (30)

製品の概要

  • 製品名

    Anti-HA tag antibody [4C12]
    HA tag 一次抗体 製品一覧

  • 製品の詳細

    Mouse monoclonal [4C12] to HA tag

  • 由来種

    Mouse

  • 特異性

    The antibody detects HA-tag fused to the C-terminus or N-terminus of targeted proteins in transfected cells. The antibody works in ChIP but less well than ab9110 (see ChIP data).

  • アプリケーション

    適用あり: ELISA, ICC/IF, ChIP, IP, WBmore details

  • 種交差性

    交差種: Species independent

  • ポジティブ･コントロール

    • WB: Rab6-HA transfected HEK-293T whole cell lysate. IP: Rab6-HA transfected HEK-293T whole cell lysate. ICC/IF: Baby hamster kidney cell expressing HA-tag CFTR protein. ChIP: Xenopus laevis oocytes injected with mRNA for HA-tagged human BORIS.

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

  • バッファー

    PBS pH7.2 with 50% glycerol, 1% BSA and 0.02% sodium azide
  • Concentration information loading...

  • ポリ/モノ

    モノクローナル

  • クローン名

    4C12

  • アイソタイプ

    IgG1

  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab1424 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ELISA 1/1000. PubMed: 17167031
ICC/IF Use a concentration of 10 µg/ml.
ChIP Use a concentration of 3 µg/ml. Use 3 µg per 25 µg chromatin (see figure legend).
IP Use a concentration of 10 µg/ml.
WB Use a concentration of 1 µg/ml.

ターゲット情報

  • 関連性

    Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins.

  • 別名

    • HA epitope tag antibody
    • HA1 antibody
    • HA2 antibody
    • hemagglutinin antibody
    • Hemagglutinin HA1 chain antibody
    • Hemagglutinin HA2 chain antibody
    see all

画像

  • Western blot - Anti-HA tag antibody [4C12] (ab1424)
    Western blot - Anti-HA tag antibody [4C12] (ab1424)This image is courtesy of an anonymous Abreview
    Anti-HA tag antibody [4C12] (ab1424) at 1/1000 dilution + HEK293T whole cell lysate over-expressing HA-tagged Rab6 at 20 µg

    Secondary
    HRP-conjugated goat anti-mouse polyclonal IgG at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Exposure time: 5 seconds


    Blocked with 5% milk for 30 min at 25°C

    See Abreview

  • Immunocytochemistry/ Immunofluorescence - Anti-HA tag antibody [4C12] (ab1424)
    Immunocytochemistry/ Immunofluorescence - Anti-HA tag antibody [4C12] (ab1424)This image was kindly supplied as part of the review submitted by one of our customers.

    This picture shows baby hamster kidney cell expressing HA-tag CFTR protein. This type of cells were grown on coverslip and immunofluorescence was performed. Dilution of 1:200 was used and incubated for 2hrs with antibody.

  • Immunoprecipitation - Anti-HA tag antibody [4C12] (ab1424)
    Immunoprecipitation - Anti-HA tag antibody [4C12] (ab1424)Data courtesy of an anonymous AbReview

    Recombinant HA-tagged Rab6 was immunoprecipitated from 1 mg transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract with 2 μL ab1424. 

    Lane 1: Rab6-HA transfected HEK-293T whole cell extract (Input)

    Lane 2: ab1424 IP in Rab6-HA transfected HEK-293T whole cell extract.

     

  • ChIP - Anti-HA tag antibody [4C12] (ab1424)
    ChIP - Anti-HA tag antibody [4C12] (ab1424)

    Xenopus laevis oocytes were injected with mRNA for HA-tagged human BORIS. Chromatin was prepared according to the Abcam X-ChIP protocol. Oocytes were fixed with formaldehyde for 10min. The  ChIP was performed with 25µg of chromatin, 3µg of ab1424 (anti-HA, light blue)  and 3µg of ab18337 (anti-BORIS, dark blue), and 20µl of Protein A/G sepharose beads. A non-specific antibody was used as a control (yellow). The immunoprecipitated DNA was quantified by real time PCR (Taqman approach).

参考文献

This product has been referenced in:

  • Peng J  et al. Drosophila Fezf coordinates laminar-specific connectivity through cell-intrinsic and cell-extrinsic mechanisms. Elife 7:N/A (2018). Read more (PubMed: 29513217) »
  • Chang YC  et al. Temporal and spatial order of photoreceptor and glia projections into optic lobe in Drosophila. Sci Rep 8:12669 (2018). Read more (PubMed: 30140062) »
See all 30 Publications for this product

ab1424 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

レビューと Q&A

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1-10 of 25 Abreviews or Q&A

Immunoprecipitation abreview for Anti-HA tag antibody [4C12]

 Excellent
Abreviews
abreview image
Application
Immunoprecipitation
Sample
Human Cell lysate - whole cell (Human osteosarcoma)
Total protein in input
1000 µg
Treatment
Plasmid transfection for 24 hrs
Immuno-precipitation step
Protein A/G
Specification
Human osteosarcoma
Read More

Miss. Can Zhou

Verified customer

投稿 Jan 28 2016

Western blot abreview for Anti-HA tag antibody [4C12]

 Excellent
Abreviews
abreview image
Application
Western blot
Sample
Mouse Cell lysate - whole cell (Myoblast)
Gel Running Conditions
Non-reduced Denaturing (10% gel)
Loading amount
40 µg
Treatment
Plasmid transfection
Specification
Myoblast
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 18°C
Read More

Abcam user community

Verified customer

投稿 Jan 28 2016

Immunocytochemistry/ Immunofluorescence abreview for Anti-HA tag antibody [4C12]

 Good
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (vascular smooth muscle cell)
Permeabilization
Yes - NP40
Specification
vascular smooth muscle cell
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 21°C
Fixative
Formaldehyde
Read More

Abcam user community

Verified customer

投稿 Jan 06 2016

Western blot abreview for Anti-HA tag antibody [4C12]

 Average
Abreviews
abreview image
Application
Western blot
Loading amount
20 µg
Gel Running Conditions
Reduced Denaturing (10)
Sample
Human Cell lysate - whole cell (HEK293)
Specification
HEK293
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Read More

Abcam user community

Verified customer

投稿 Mar 03 2015

Immunoprecipitation abreview for Anti-HA tag antibody [4C12] - ChIP Grade

 Good
Abreviews
abreview image
Application
Immunoprecipitation
Sample
Human Cell lysate - whole cell (Retro virus transduction for protein X)
Total protein in input
500 µg
Specification
Retro virus transduction for protein X
Immuno-precipitation step
Protein A/G
Read More

Abcam user community

Verified customer

投稿 Feb 26 2013

Immunoprecipitation abreview for Anti-HA tag antibody [4C12] - ChIP Grade

 Good
Abreviews
abreview image
Application
Immunoprecipitation
Sample
Human Cell lysate - whole cell (HEK 293T cells)
Total protein in input
1000 µg
Specification
HEK 293T cells
Immuno-precipitation step
Protein A
Read More

Abcam user community

Verified customer

投稿 Jan 16 2013

Western blot abreview for Anti-HA tag antibody [4C12] - ChIP Grade

 Excellent
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (HEK293T)
Loading amount
20 µg
Specification
HEK293T
Gel Running Conditions
Reduced Denaturing (12% Tris-glycine gel)
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Read More

Abcam user community

Verified customer

投稿 Sep 17 2012

Question

Thanks very much for your quick reply. I'm interested in the testing discount program you mentioned. Could you please let me know more about it?

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Answer

Thank you for your reply.

You can find out more about the 100% Abreview Testing Discount Program below:

https://www.abcam.com/index.html?pageconfig=resource&rid=11998&viapagetrap=collaborationdiscount



For all the antibodies that you are interested in, we already guarantee them to work in ChIP experiments, regardless of species. Therefore, they would not qualify for the testing discount program, but they are covered under our Abpromise.

If there is anything else I can help you with, or if you are interested in any of our other antibodies that have not been tested in ChIP, but you might be interested in them, then please let me know.

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Question

Dear Sir/Madam, I'm doing a ChIP experiment using drosophila tissue. Your ChIP-grade anti-epitope tag antibodies may be very helpful for me. The Abcam antibodies usually work well for us, so I would like to give it a try. But before we place a order, I'm wondering whether I can get a small aliquot of the antibodies to see whether they would work for my tissue. I'm particularly interested in AbID 9110 (or 1424) and 9132. Please let me know. hank you very much in advance!

Read More
Answer

Thank you for contacting Abcam. Because we carry over 90,000 products, it isn't feasible for us to keep small sample sizes of our products.

We are happy to reassure our customers that all of our products are covered by our Abpromise, which guarantees that the product will work in the applications and species specified on the datasheet, or we will offer a replacement, credit, or refund within 6 months of purchase.

If the product is to be used in an untested species or application, you may be eligible for our testing discount program if the antibody has not yet been purchased. Please contact our Scientific Support team by replying to this email prior to purchase for more information.

Otherwise, we like to encourage all of our customers to submit an Abreview via the online product datasheet. We always appreciate customer feedback, whether positive or negative, and we make all product information available to researchers. Plus, each Abreview earns Abpoints that can be used for discounts on future purchases or rewards such as Amazon.com gift certificates.

To find out more about our Abreview system, please see the following link:

https://www.abcam.com/abreviews

I hope this information is helpful. Please do not hesitate to contact us again with any other questions.

Read More

Question

Our lab is trying to use these antibodies (ab9110 and ab1424) to do immunohistochemistry on sections from various mouse organs. We were wondering if these antibodies are tested and approved for use on paraffin sections only, cryopreserved sections only, or both?

Read More
Answer

Thank you for contacting us. Both antibodies have not yet been tested in any IHC application (neither IHC-P or IHC-Fr). We do have a testing discount program that you can use in that case. Here is some information about how the testing discount program works: The testing discount program is for customers who like to use an antibody/protein on an untested species/application. You would purchase the antibody at full price, test it and submit an Abreview with your data (positive or negative). On your next order you will receive a discount for ONE antibody at the full price (100%) of the antibody you have tested. The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount. I'd be happy to issue you a discount code for one or both antibodies. Please let me know. Also, please do not hesitate to contact us if you need any more advice or information.

Read More

1-10 of 25 Abreviews or Q&A

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