Application
Immunoprecipitation
Sample
Human Cell lysate - whole cell (Human osteosarcoma)
Total protein in input
1000 µg
Treatment
Plasmid transfection for 24 hrs
Immuno-precipitation step
Protein A/G
Specification
Human osteosarcoma
Other product details
Incubation time
14 hour(s) and 0 minute(s) · Temperature: 4°C
Concentration
5 µg/mg lysate
Additional data
Additional Notes
For each group, HA-KLC2 was co-transfected with either GFP only, GFP-nesprin WT, or GFP-nesprin mutant.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
MISS. Can Zhou
Verified customer
投稿 Jan 28 2016