Anti-GTF2H1 抗体 (ab272022)
Key features and details
- Rabbit polyclonal to GTF2H1
- Suitable for: WB, IP, IHC-P
- Reacts with: Human
- Isotype: IgG
製品の概要
-
製品名
Anti-GTF2H1 antibody
GTF2H1 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to GTF2H1 -
由来種
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanIP HumanWB Human
-
免疫原
Synthetic peptide within Human GTF2H1 aa 250-300. The exact sequence is proprietary. NP_001135779.1
Database link: P32780 -
ポジティブ･コントロール
- WB: HEK-293T, Jurkat and HeLa whole cell lysate. IP: HEK-293T whole cell lysate. IHC-P: Human testis tissue.
-
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
-
Compatible Secondaries
-
Positive Controls
アプリケーション
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab272022 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
| アプリケーション | Species |
|---|---|
| IHC-P |
Human
|
| IP |
Human
|
| WB |
Human
|
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/2000 - 1/10000. Predicted molecular weight: 62 kDa.
|
|
| IP |
Use at 2-10 µg/mg of lysate.
|
|
| IHC-P |
1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
| 特記事項 |
|---|
|
WB
1/2000 - 1/10000. Predicted molecular weight: 62 kDa. |
|
IP
Use at 2-10 µg/mg of lysate. |
|
IHC-P
1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ターゲット情報
-
機能
Component of the core-TFIIH basal transcription factor involved in nucleotide excision repair (NER) of DNA and, when complexed to CAK, in RNA transcription by RNA polymerase II. -
配列類似性
Contains 2 BSD domains. -
細胞内局在
Nucleus. - Information by UniProt
-
参照データベース
- Entrez Gene: 2965 Human
- Omim: 189972 Human
- SwissProt: P32780 Human
- Unigene: 577202 Human
-
別名
- Basic transcription factor 2 62 kDa subunit antibody
- Basic transcription factor 62 kDa subunit antibody
- BTF 2 antibody
see all
画像
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GTF2H1 antibody (ab272022)
Immunohistochemical analysis of formalin-fixed, paraffin-embedded human testis tissue labeling GTF2H1 with ab272022 at a 1/1,000 dilution. Detection: DAB.
-
Western blot - Anti-GTF2H1 antibody (ab272022)All lanes : Anti-GTF2H1 antibody (ab272022) at 0.1 µg/ml
Lane 1 : HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg
Lane 2 : HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 15 µg
Lane 3 : Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate at 50 µg
Lane 4 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg
Predicted band size: 62 kDa
Exposure time: 3 minutes -
Immunoprecipitation - Anti-GTF2H1 antibody (ab272022)GTF2H1 was immunoprecipitated from HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab272022 at 6 μg/mg lysate. WB was performed using ab272022 at 1 µg/ml.
Lane 1: ab272022 IP in HEK-293T whole cell lysate.
Lane 2: Control IgG IP.
Exposure time: 10 seconds.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (0)
ab272022 は論文での使用が確認できていません。