Anti-GST 抗体 [EPR4236] - BSA and Azide free (ab235384)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR4236] to GST - BSA and Azide free
- Suitable for: WB, IP, ICC/IF
- Reacts with: Species independent
Related conjugates and formulations
製品の概要
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製品名
Anti-GST antibody [EPR4236] - BSA and Azide free
GST 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR4236] to GST - BSA and Azide free -
由来種
Rabbit -
アプリケーション
適用あり: WB, IP, ICC/IFmore details
適用なし: Flow Cyt or IHC-P -
種交差性
交差種: Species independent -
免疫原
Synthetic peptide (GST-tag) within GST aa 300-450. The exact sequence is proprietary. GST tag was fused to ATG16L1 to produce a recombinant protein.
Database link: Q676U5 -
特記事項
ab235384 is the carrier-free version of ab111947.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
バッファー
pH: 7.2
Constituent: PBS -
キャリア・フリー
はい -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR4236 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Conjugation kits
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab235384の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
WB |
Use at an assay dependent concentration. Predicted molecular weight: 26 kDa.
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IP |
Use at an assay dependent concentration.
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ICC/IF |
Use at an assay dependent concentration.
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特記事項 |
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WB
Use at an assay dependent concentration. Predicted molecular weight: 26 kDa. |
IP
Use at an assay dependent concentration. |
ICC/IF
Use at an assay dependent concentration. |
ターゲット情報
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関連性
GST (Glutathione S-Transferase) is a 26kDa protein encoded by the parasitic helminth Schistosoma japonicum and widely used in the pGEX family of GST plasmid expression vectors as a fusion protein with foreign proteins. -
別名
- Glutathione S Transferase antibody
- Glutathione S-transferase class-mu 26 kDa isozyme antibody
- GST antibody
see all
画像
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(Cross IP) GST immunoprecipitation from transfected 293T whole cell lysate using ab111947. 1 mg of cell lysate was incubated with capture antibody (ab111947) at 1/20 dilution.
Lane 1: Primary: ab180123, 1/1000 dilution
Sample: 293T transfected with GST tagged protein vector cell lysate 10 ug
Lane 2: Primary: ab180123, 1/1000 dilution
Sample: 293T transfected with GST tagged protein vector cell lysate
Secondary: ab131366, Veriblot, 1/10000 dilution
Lane 3: Primary: ab172730, Rabbit monoclonal IgG
Sample: 293T transfected with GST tagged protein vector cell lysateAll lanes: VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10,000 dilution
Blocking and diluting buffer: 5% NFDM/TBSTThis data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab111947).
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GST immunoprecipitation from transfected 293T whole cell lysate using ab111947. 1 mg of cell lysate was incubated with capture antibody (ab111947) at 1/20 dilution.
Lane 1: Primary: ab111947, 1/1000 dilution
Sample: 293T transfected with GST tagged protein vector cell lysate 10 ug
Lane 2: Primary: ab111947, 1/1000 dilution
Sample: 293T transfected with GST tagged protein vector cell lysate
Lane 3: Primary: ab172730, Rabbit monoclonal IgG
Sample: 293T transfected with GST tagged protein vector cell lysateAll lanes: VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10,000 dilution
Blocking and diluting buffer: 5% NFDM/TBSTThis data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab111947).
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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Datasheet download
Certificate of Compliance
参考文献 (0)
ab235384 は論文での使用が確認できていません。