Anti-GST 抗体 (ab9085)
Key features and details
- Rabbit polyclonal to GST
- Suitable for: WB
- Reacts with: Species independent
- Isotype: IgG
製品の概要
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製品名
Anti-GST antibody
GST 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to GST -
由来種
Rabbit -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Species independent -
免疫原
The details of the immunogen for this antibody are not available.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7.00
Constituent: 50% Glycerol -
Concentration information loading... -
精製度
Immunogen affinity purified -
特記事項（精製）
Affinity purified against bacterially expressed GST -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab9085の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | (2) |
1/500.
|
| 特記事項 |
|---|
|
WB
1/500. |
ターゲット情報
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関連性
GST (Glutathione S-Transferase) is a 26kDa protein encoded by the parasitic helminth Schistosoma japonicum and widely used in the pGEX family of GST plasmid expression vectors as a fusion protein with foreign proteins. -
別名
- Glutathione S Transferase antibody
- Glutathione S-transferase class-mu 26 kDa isozyme antibody
- GST antibody
see all
画像
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Western blot - Anti-GST antibody (ab9085)This image is courtesy of an anonymous AbreviewAll lanes : Anti-GST antibody (ab9085) at 1/2000 dilution
Lane 1 : Recombinant GST protein purified from E. coli XL1-Blue
Lane 2 : Recombinant GST-tagged p53 protein purified from E. coli XL1-Blue
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP-conjugated Goat anti-Rabbit IgG polyclonal at 1/20000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Observed band size: 26,80 kDa why is the actual band size different from the predicted?
Exposure time: 5 seconds
Blocked with 5% Milk for 1 hour at 25°C.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (56)
ab9085 は 56 報の論文で使用されています。
- Peng H et al. Kinetic Characterization of ASXL1/2-Mediated Allosteric Regulation of the BAP1 Deubiquitinase. Mol Cancer Res 19:1099-1112 (2021). PubMed: 33731362
- Park SY et al. Reduction in MLKL-mediated endosomal trafficking enhances the TRAIL-DR4/5 signal to increase cancer cell death. Cell Death Dis 11:744 (2020). PubMed: 32917855
- Cloutier P et al. Upstream ORF-Encoded ASDURF Is a Novel Prefoldin-like Subunit of the PAQosome. J Proteome Res 19:18-27 (2020). PubMed: 31738558
- Amano G et al. SCYL1 arginine methylation by PRMT1 is essential for neurite outgrowth via Golgi morphogenesis. Mol Biol Cell 31:1963-1973 (2020). PubMed: 32583741
- Kato S et al. Gain-of-Function Genetic Alterations of G9a Drive Oncogenesis. Cancer Discov 10:980-997 (2020). PubMed: 32269030