Anti-GST 抗体 (ab9085)

Reviews (2)Q&A (4)References (49)

Key features and details

  • Rabbit polyclonal to GST
  • Suitable for: ELISA, IP, WB
  • Reacts with: Species independent
  • Isotype: IgG

製品の概要

  • 製品名

    Anti-GST antibody
    GST 一次抗体 製品一覧

  • 製品の詳細

    Rabbit polyclonal to GST

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: ELISA, IP, WBmore details

  • 種交差性

    交差種: Species independent

  • 免疫原

    Rabbits were immunized with Glutathione-S-transferase (GST) from Schistosoma japonicum.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab9085 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ELISA 1/1000.
IP Use at an assay dependent concentration.
WB 1/500.

ターゲット情報

  • 関連性

    GST (Glutathione S-Transferase) is a 26kDa protein encoded by the parasitic helminth Schistosoma japonicum and widely used in the pGEX family of GST plasmid expression vectors as a fusion protein with foreign proteins.

  • 別名

    • Glutathione S Transferase antibody
    • Glutathione S-transferase class-mu 26 kDa isozyme antibody
    • GST antibody
    • Sj26 antigen antibody
    • SjGST antibody
    see all

画像

  • Western blot - Anti-GST antibody (ab9085)
    Western blot - Anti-GST antibody (ab9085)This image is courtesy of an anonymous Abreview
    All lanes : Anti-GST antibody (ab9085) at 1/2000 dilution

    Lane 1 : Recombinant GST protein purified from E. coli XL1-Blue
    Lane 2 : Recombinant GST-tagged p53 protein purified from E. coli XL1-Blue

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP-conjugated Goat anti-Rabbit IgG polyclonal at 1/20000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Observed band size: 26,80 kDa
    why is the actual band size different from the predicted?


    Exposure time: 5 seconds


    Blocked with 5% Milk for 1 hour at 25°C.

    See Abreview

参考文献 (49)

ab9085 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab9085 は 49 報の論文で使用されています。

  • Sugiyama Y  et al. A Rho-actin signaling pathway shapes cell wall boundaries in Arabidopsis xylem vessels. Nat Commun 10:468 (2019). PubMed: 30692538
  • Rao RA  et al. KMT1 family methyltransferases regulate heterochromatin-nuclear periphery tethering via histone and non-histone protein methylation. EMBO Rep 20:N/A (2019). PubMed: 30858340
  • Wojnarowicz PM  et al. A Small-Molecule Pan-Id Antagonist Inhibits Pathologic Ocular Neovascularization. Cell Rep 29:62-75.e7 (2019). PubMed: 31577956
  • Kawakami Y  et al. The Soluble Form of LOTUS inhibits Nogo Receptor-Mediated Signaling by Interfering with the Interaction Between Nogo Receptor Type 1 and p75 Neurotrophin Receptor. J Neurosci 38:2589-2604 (2018). PubMed: 29440387
  • Malik I  et al. Development of a Cell-Based Assay to Assess Binding of the proNGF Prodomain to Sortilin. Cell Mol Neurobiol 38:827-840 (2018). ICC . PubMed: 29067536
View all Publications for this product

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1-6 of 6 Abreviews or Q&A

Western blot abreview for Anti-GST antibody

 Excellent
Abreviews
abreview image
Application
Western blot
Sample
Human Recombinant protein (E. coli-purified GST)
Loading amount
0.05 µg
Specification
E. coli-purified GST
Gel Running Conditions
Reduced Denaturing (10% Acrylamide)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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Abcam user community

Verified customer

投稿 Feb 21 2013

Question

Hi, I am working in UTSW, and try to look for an antibody GST working in IF, could your company antibody Anti-GST antibody (ab6613) work in IF or did you test it in IHC?   Thanks so much.  

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Answer

Thank you for contacting us.

While it is likely that this product will work in IF, we have not yet tested ab6613 in this application and therefore cannot guarantee that it will give the desired results in this application. We do have a number of anti-GST antibodies which have been tested and are covered by our Abpromise guarantee for use in IF. Two of the better characterized are:

ab9085 https://www.abcam.com/GST-antibody-ab9085.html

ab18183https://www.abcam.com/GST-antibody-GST-B6-ab18183.html



I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Use our products? Submit an Abreview. Earn rewards!
https://www.abcam.com/abreviews

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Question

Thank you for your help and prompt attention to this matter.

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Answer

Our product team has created this form which I have attached. This should be live on the website as well. Please let me know if you have any other questions or if there is anything else Abcam might do for you.

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Question

Good morning-   I am trying to locate an MSDS or exemption letter for a product called Anti-GST Antibody; item number ab9085.  If you could send me a copy it would be greatly appreciated.  This product is being used in a VA Medical Center in Minneapolis, MN.   If you have any questions or need any further information please feel free to contact me using the information below.

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Answer

Thank you for contacting us.

I have contacted our products team and they are currently creating an MSDS for this product. This should be on the website soon. I will send you a copy when this has been created as well.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Use our products? Submit an Abreview. Earn rewards!
https://www.abcam.com/abreviews

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Western blot abreview for Anti-GST antibody

 Excellent
Abreviews
abreview image
Application
Western blot
Sample
Escherichia coli Purified protein (Recombinant protein purified from E. coli XL1-Blue)
Loading amount
10 µg
Specification
Recombinant protein purified from E. coli XL1-Blue
Gel Running Conditions
Reduced Denaturing (12 % PAGE gel)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Read More

Abcam user community

Verified customer

投稿 Jun 08 2012

Question

I would like to know if this antibody detects all forms of GST. Thank you.

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Answer

I am sorry for the delay in replying to you. I finally got a confirmation from the originator of the product. According to them, as far as they know, this product will detect all forms of GST. If there is anything else that I can help you with, please do not hesitate to contact me.

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