Application
Western blot
Sample
Human Cell lysate - whole cell (Lysate from Insect Cells Expressing RGS9-GST fusio)
Loading amount
100 µg
Specification
Lysate from Insect Cells Expressing RGS9-GST fusio
Gel Running Conditions
Reduced Denaturing (10% Tris Glycine)
Blocking step
1:1 PBS/Licor Blocking Buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 50% · Temperature: 22°C
Other product details
Dilution
1/1000
Incubation time
14 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 1:1 PBS/Licor Blocking Buffer
Secondary antibody
Name
Non-Abcam antibody was used: IRDye 800CW Goat anti-Mouse IgG (H + L)
Host species: Mouse
Clonality: Monoclonal
Conjugation: IRDye® 800CW
Host species: Mouse
Clonality: Monoclonal
Conjugation: IRDye® 800CW
Dilution
1/10000
Detection
Detection method
Odyssey Infrared Imager
Exposure
3 minute(s) and 0 second(s)
Bands
Specific: 80 kDa
Additional data
Additional Notes
Lane 1 MW Ladder, Lane 2 and 3 are identical lysates from Insect Cells Expressing Mouse RGS9 Fused to Human GST at ~80 KD. Any nonspecific bands are too light to consider.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Dr. Chris Tracy
Verified customer
投稿 Sep 20 2011