Anti-GST 抗体 [3G10/1B3] (ab92)
![Western blot - Anti-GST antibody [3G10/1B3] (ab92)](https://www.abcam.co.jp/ps/products/0/ab92/Images/ab92-230826-anti-gst-antibody-3g10-1b3-western-blot.jpg "Western blot - Anti-GST antibody [3G10/1B3] (ab92)")
Key features and details
- Mouse monoclonal [3G10/1B3] to GST
- Suitable for: WB, IP
- Reacts with: Species independent
- Isotype: IgG1
製品の概要
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製品名
Anti-GST antibody [3G10/1B3]
GST 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [3G10/1B3] to GST -
由来種
Mouse -
特異性
Recognizes Glutathione S-Transferase (GST), a 26-kDa protein encoded by the parasitic helminth Schistosoma japonicum. -
アプリケーション
適用あり: WB, IPmore details -
種交差性
交差種: Species independent -
免疫原
Recombinant full length protein within Schistosoma japonicum GST aa 1-218. The exact sequence is proprietary. This is full length GST fusion protein of 425 aa (length of the fusion protein, not GST protein alone). The GST protein is the same GST protein on the pGEX vector ( Schistosoma japonicum).
Database link: P08515 -
特記事項
This product was changed from ascites to tissue culture supernatant on 25th May 2018. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7.40
Constituent: 100% PBS
w/o preservative -
Concentration information loading... -
精製度
Protein G purified -
ポリ/モノ
モノクローナル -
クローン名
3G10/1B3 -
ミエローマ
NS1 -
アイソタイプ
IgG1 -
軽鎖の種類
kappa -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab92の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | (3) |
Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 30 kDa (predicted molecular weight: 26 kDa).
|
| IP |
Use at an assay dependent dilution.
|
| 特記事項 |
|---|
|
WB
Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 30 kDa (predicted molecular weight: 26 kDa). |
|
IP
Use at an assay dependent dilution. |
ターゲット情報
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関連性
GST (Glutathione S-Transferase) is a 26kDa protein encoded by the parasitic helminth Schistosoma japonicum and widely used in the pGEX family of GST plasmid expression vectors as a fusion protein with foreign proteins. -
別名
- Glutathione S Transferase antibody
- Glutathione S-transferase class-mu 26 kDa isozyme antibody
- GST antibody
see all
画像
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Western blot - Anti-GST antibody [3G10/1B3] (ab92)All lanes : Anti-GST antibody [3G10/1B3] (ab92) at 1/5000 dilution
Lane 1 : Non-induced GST-fusion protein expressed in E.coli
Lane 2 : IPTG induced GST-fusion protein expressed in E.coli
Lysates/proteins at 5 µl per lane.
Predicted band size: 26 kDa10% SDS-PAGE
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![Western blot - Anti-GST antibody [3G10/1B3] (ab92)](https://www.abcam.co.jp/ps/products/0/ab92/Images/ab92-3379-anti-gst-antibody-3g10-1b3-western-blot.jpg)
Western blot - Anti-GST antibody [3G10/1B3] (ab92)All lanes : Anti-GST antibody [3G10/1B3] (ab92)
Lane 1 : Total lysate
Lane 2 : Cytoplasmic fraction
Lane 3 : Membrane fraction
Lane 4 : Nuclear fraction
Predicted band size: 26 kDa
Detection of human GST in cytoplasmic fraction by anti-GST 3G10 monoclonal antibody (ab92) in western blot experiment. p84 is also shown indicating the nuclear fraction using anti-p84 monoclonal antibody (ab487).
プロトコール
データシートおよび資料
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Datasheet download
参考文献 (24)
ab92 は 24 報の論文で使用されています。
- Zhang X et al. PTPN22 interacts with EB1 to regulate T-cell receptor signaling. FASEB J 34:8959-8974 (2020). PubMed: 32469452
- Marquardt J et al. The LKB1-like Kinase Elm1 Controls Septin Hourglass Assembly and Stability by Regulating Filament Pairing. Curr Biol 30:2386-2394.e4 (2020). PubMed: 32386534
- Geymonat M et al. Orderly assembly underpinning built-in asymmetry in the yeast centrosome duplication cycle requires cyclin-dependent kinase. Elife 9:N/A (2020). PubMed: 32851976
- Pantier R et al. TET1 Interacts Directly with NANOG via Independent Domains Containing Hydrophobic and Aromatic Residues. J Mol Biol 432:6075-6091 (2020). PubMed: 33058869
- He Y et al. The OsGSK2 Kinase Integrates Brassinosteroid and Jasmonic Acid Signaling by Interacting with OsJAZ4. Plant Cell 32:2806-2822 (2020). PubMed: 32586913
