Anti-GSK3 beta (phospho S9) 抗体 (ab131097)
Key features and details
- Rabbit polyclonal to GSK3 beta (phospho S9)
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-GSK3 beta (phospho S9) antibody
GSK3 beta 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to GSK3 beta (phospho S9) -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-P, ICC/IFmore details -
種交差性
交差種: Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ・コントロール
- 293 cell extract treated with serum; HeLa cells; Human breast carcinoma and lung carcinoma tissues.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 49% PBS, 50% Glycerol, 0.88% Sodium chloride
PBS is without Mg2+ and Ca2+ -
Concentration information loading...
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精製度
Immunogen affinity purified -
特記事項(精製)
ab131097 was purified by affinity chromatography using epitope specific phosphopetide. Non phospho specific antibodies were removed by chromatography using non phosphopetide. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab131097の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/500 - 1/1000. Predicted molecular weight: 46 kDa.
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IHC-P |
1/50 - 1/100.
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ICC/IF |
1/100 - 1/200.
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特記事項 |
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WB
1/500 - 1/1000. Predicted molecular weight: 46 kDa. |
IHC-P
1/50 - 1/100. |
ICC/IF
1/100 - 1/200. |
ターゲット情報
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機能
Participates in the Wnt signaling pathway. Implicated in the hormonal control of several regulatory proteins including glycogen synthase, MYB and the transcription factor JUN. Phosphorylates JUN at sites proximal to its DNA-binding domain, thereby reducing its affinity for DNA. Phosphorylates MUC1 in breast cancer cells, and decreases the interaction of MUC1 with CTNNB1/beta-catenin. Phosphorylates CTNNB1/beta-catenin. Phosphorylates SNAI1. Plays an important role in ERBB2-dependent stabilization of microtubules at the cell cortex. Prevents the phosphorylation of APC and CLASP2, allowing its association with the cell membrane. In turn, membrane-bound APC allows the localization of MACF1 to the cell membrane, which is required for microtubule capture and stabilization. Phosphorylates MACF1 and this phosphorylation inhibits the binding of MACF1 to microtubules which is critical for its role in bulge stem cell migration and skin wound repair. -
組織特異性
Expressed in testis, thymus, prostate and ovary and weakly expressed in lung, brain and kidney. -
配列類似性
Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. GSK-3 subfamily.
Contains 1 protein kinase domain. -
翻訳後修飾
Phosphorylated by AKT1 and ILK1. Activated by phosphorylation at Tyr-216. -
細胞内局在
Cytoplasm. Nucleus. Cell membrane. The phosphorylated form shows localization to cytoplasm and cell membrane. The MEMO1-RHOA-DIAPH1 signaling pathway controls localization of the phosophorylated form to the cell membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 2932 Human
- Omim: 605004 Human
- SwissProt: P49841 Human
- Unigene: 445733 Human
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別名
- Glycogen Synthase Kinase 3 Beta antibody
- Glycogen synthase kinase-3 beta antibody
- GSK 3 beta antibody
see all
画像
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All lanes : Anti-GSK3 beta (phospho S9) antibody (ab131097) at 1/500 dilution
Lane 1 : 293 cell extract, untreated
Lane 2 : 293 cell extract treated with serum
Predicted band size: 46 kDa -
Immunohistochemical staining of GSK3 beta (phospho S9) in paraffin embedded Human breast carcinoma tissue, using ab131097 at a 1/50 dilution.
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Immunofluorescence analysis of methanol fixed HeLa cells staining GSK3 beta (phospho S9) with ab131097 at a dilution of 1/100.
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Methanol-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for GSK3 beta (phospho S9) (red) using ab131097 at 1/100 dilution in ICC/IF.
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Paraffin-embedded human lung carcinoma tissue stained for GSK3 beta (phospho S9) using ab131097 at 1/50 dilution in immunohistochemical analysis.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (74)
ab131097 は 74 報の論文で使用されています。
- Sun Y et al. CDK14/β-catenin/TCF4/miR-26b positive feedback regulation modulating pancreatic cancer cell phenotypes in vitro and tumor growth in mice model in vivo. J Gene Med 24:e3343 (2022). PubMed: 33871149
- Silva-Aguiar RP et al. Albumin Expands Albumin Reabsorption Capacity in Proximal Tubule Epithelial Cells through a Positive Feedback Loop between AKT and Megalin. Int J Mol Sci 23:N/A (2022). PubMed: 35055044
- Wang P et al. MicroRNA-1246 suppresses the metastasis of breast cancer cells by targeting the DYRK1A/PGRN axis to prevent the epithelial-mesenchymal transition. Mol Biol Rep 49:2711-2721 (2022). PubMed: 35059968
- Shi YL et al. Trilobatin, a Natural Food Additive, Exerts Anti-Type 2 Diabetes Effect Mediated by Nrf2/ARE and IRS-1/GLUT2 Signaling Pathways. Front Pharmacol 13:828473 (2022). PubMed: 35153796
- Bukovac A et al. Bilateral Meningioma: A Case Report and Review of the Literature. Int J Mol Sci 23:N/A (2022). PubMed: 35163107