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    gsk3-alpha--beta-phospho-y216--y279-antibody-ab4797.pdf

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Anti-GSK3 (alpha + beta) (phospho Y216 + Y279) 抗体 (ab4797)

  • Datasheet
Reviews (4) Submit a question References (9)

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Western blot - Anti-GSK3 (alpha + beta) (phospho Y216 + Y279) antibody (ab4797)

    Key features and details

    • Rabbit polyclonal to GSK3 (alpha + beta) (phospho Y216 + Y279)
    • Suitable for: WB
    • Reacts with: Mouse
    • Isotype: IgG

    リコンビナント抗体で、ロット間での高い再現性を実現

    Product image
    Anti-GSK3 beta (phospho Y216) + GSK3 alpha (phospho Y279) antibody [EPR933Y] (ab68476)
    • 異なるロット間での安定した再現性
    • 容易なスケールアップ
    • 評価試験による特異性の確認済み
    • 倫理基準に準拠 - アニマル・フリーの生産

    製品の概要

    • 製品名

      Anti-GSK3 (alpha + beta) (phospho Y216 + Y279) antibody
      GSK3 (alpha + beta) 一次抗体 製品一覧
    • 製品の詳細

      Rabbit polyclonal to GSK3 (alpha + beta) (phospho Y216 + Y279)
    • 由来種

      Rabbit
    • アプリケーション

      適用あり: WBmore details
    • 種交差性

      交差種: Mouse
    • 免疫原

      Synthetic peptide corresponding to GSK3 (alpha + beta) (phospho Y216 + Y279). The sequence is conserved in rat and zebrafish GSK-3a and mouse, rat, frog and zebrafish GSK-3b.

    • 特記事項


      Glycogen synthase kinase-3 (GSK 3) is a proline-directed serine-threonine kinase that was initially identified as a phosphorylating and inactivating glycogen synthase. Two isoforms, alpha (GSK 3A) and beta, show a high degree of amino acid homology. GSK 3B is involved in energy metabolism, neuronal cell development, and body pattern formation.

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    製品の特性

    • 製品の状態

      Liquid
    • 保存方法

      Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
    • バッファー

      pH: 7.3
      Constituent: PBS
    • Concentration information loading...
    • 精製度

      Immunogen affinity purified
    • 特記事項(精製)

      Purified from rabbit serum by epitope specific affinity chromatography. Any reactivity towards the non-tyrosine phosphorylated GSK 3 alpha + beta protein has been eliminated through a series of preabsorption steps.
    • 一次抗体 備考

      Glycogen synthase kinase-3 (GSK 3) is a proline-directed serine-threonine kinase that was initially identified as a phosphorylating and inactivating glycogen synthase. Two isoforms, alpha (GSK 3A) and beta, show a high degree of amino acid homology. GSK 3B is involved in energy metabolism, neuronal cell development, and body pattern formation.
    • ポリ/モノ

      ポリクローナル
    • アイソタイプ

      IgG
    • 研究分野

      • Neuroscience
      • Neurology process
      • Neurodegenerative disease
      • Alzheimer's disease
      • Tangles & Tau
      • Signal Transduction
      • Protein Phosphorylation
      • Ser / Thr Kinases
      • Other Kinases
      • Stem Cells
      • Signaling Pathways
      • Wnt
      • Cytoplasmic
      • Neuroscience
      • Development

    関連製品

    • Compatible Secondaries

      • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
      • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Isotype control

      • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)

    アプリケーション

    The Abpromise guarantee

    Abpromise保証は、 次のテスト済みアプリケーションにおけるab4797の使用に適用されます

    アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

    アプリケーション Abreviews 特記事項
    WB (4)
    Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 51,47 kDa. Use at a concentration of 0.5 - 1.0 µg/ml. Predicted molecular weight: 51kDa for GSK 3 alpha and 47 kDa for GSK 3 beta.
    特記事項
    WB
    Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 51,47 kDa. Use at a concentration of 0.5 - 1.0 µg/ml. Predicted molecular weight: 51kDa for GSK 3 alpha and 47 kDa for GSK 3 beta.

    ターゲット情報

    • 関連性

      Glycogen synthase kinase 3 (GSK3) is a proline directed serine threonine kinase that was initially identified as a phosphorylating and inactivating glycogen synthase, a key enzyme in glycogen metabolism. Since then, it has been shown to be involved in the regulation of a diverse array of cellular functions, including protein synthesis, cell proliferation, cell differentiation, microtubule assembly/disassembly, and apoptosis. GSK3s substrate specificity is unique in that phosphorylation of substrate only occurs if a phosphoserine or phosphotyrosine is present four residues C terminal to the site of GSK phosphorylation. There exists two isoforms of GSK3, alpha and beta, and they show a high degree of amino acid homology. The two isoforms of GSK3 are strictly regulated via phosphorylation. Phosphorylation of GSK3 beta on Ser9 (Ser21 in GSK3 alpha) by protein kinase B (PKB) causes its inactivation is the primary mechanism responsible for growth factor inhibition of this kinase. Activation of GSK3 beta is dependent upon the phosphorylation of Tyr216 (Tyr279 in GSK3 alpha). Upon activation, it has been shown to phosphorylate a number of different cellular proteins, including p53, c-Myc, c-Jun, heat shock factor 1 (HSF1), and cyclin D1. GSK3 beta also has been shown to phosphorylate aberrant sites on the microtubule associated protein tau, which is critical for the progression of Alzheimer's disease. GSK3B is involved in energy metabolism, neuronal cell development, and body pattern formation.
    • 細胞内局在

      Cytoplasmic and Nuclear
    • 参照データベース

      • Entrez Gene: 56637 Mouse
      • Entrez Gene: 606496 Mouse
      • SwissProt: Q2NL51 Mouse
      • SwissProt: Q9WV60 Mouse
      • 別名

        • Factor A antibody
        • Glycogen synthase kinase 3 alpha antibody
        • Glycogen synthase kinase 3 beta antibody
        • GSK3 alpha antibody
        • GSK3 beta antibody
        • GSK3B antibody
        see all

      画像

      • Western blot - Anti-GSK3 (alpha + beta) (phospho Y216 + Y279) antibody (ab4797)
        Western blot - Anti-GSK3 (alpha + beta) (phospho Y216 + Y279) antibody (ab4797)

        Extracts of 3T3L1 cells stimulated with 100 nM insulin for 10 minutes were resolved by SDS-PAGE on a 10% Tris-glycine gel and transferred to PVDF. The membrane was blocked with a 5% BSA-TBST buffer for one hour at room temperature and either left untreated (1-4) or treated with lambda (λ) phosphatase (5), then incubated with the GSK-3 α [pY279] / β [pY216] antibody for two hours at room temperature in a 1% BSATBST buffer, following its prior incubation with: the phosphopeptide immunogen (1), no peptide (2), the non-hosphopeptide corresponding to the phosphopeptide immunogen (3), or a generic phosphotyrosine-containing peptide (4). After washing, the membrane was incubated with goat F(ab’) 2 anti-rabbit IgG HRP conjugate, and signals were detected. The data show that only the phosphopeptide corresponding to GSK-3α [pY279] /β[pY216] blocks the antibody signal, demonstrating the specificity of the antibody. The data also show that phosphatase stripping eliminates the signal, further verifying that the antibody is phospho-specific.

      プロトコール

      • Peptide Competition Experiment
      • Western blotting protocol

      Click here to view the general protocols

      データシートおよび資料

      • Datasheet download

        Download

      参考文献 (9)

      ab4797 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

      ab4797 は 9 報の論文で使用されています。

      • Rossi A  et al. Defective Mitochondrial Pyruvate Flux Affects Cell Bioenergetics in Alzheimer's Disease-Related Models. Cell Rep 30:2332-2348.e10 (2020). PubMed: 32075767
      • Vincentz JW  et al. Hand factor ablation causes defective left ventricular chamber development and compromised adult cardiac function. PLoS Genet 13:e1006922 (2017). PubMed: 28732025
      • Li XC  et al. Role of the Na+/H+ exchanger 3 in angiotensin II-induced hypertension in NHE3-deficient mice with transgenic rescue of NHE3 in small intestines. Physiol Rep 3:N/A (2015). WB . PubMed: 26564064
      • Li XC  et al. Role of the Na+/H+ exchanger 3 in angiotensin II-induced hypertension. Physiol Genomics 47:479-87 (2015). PubMed: 26242933
      • Elali A  et al. Mild chronic cerebral hypoperfusion induces neurovascular dysfunction, triggering peripheral beta-amyloid brain entry and aggregation. Acta Neuropathol Commun 1:75 (2013). WB ; Mouse . PubMed: 24252187
      • Cheng X  et al. Gestational diabetes mellitus impairs Nrf2-mediated adaptive antioxidant defenses and redox signaling in fetal endothelial cells in utero. Diabetes 62:4088-97 (2013). PubMed: 23974919
      • Jiang Y  et al. Diabetes induces changes in ILK, PINCH and components of related pathways in the spinal cord of rats. Brain Res 1332:100-9 (2010). WB ; Rat . PubMed: 20347724
      • Hartz AM  et al. Estrogen receptor beta signaling through phosphatase and tensin homolog/phosphoinositide 3-kinase/Akt/glycogen synthase kinase 3 down-regulates blood-brain barrier breast cancer resistance protein. J Pharmacol Exp Ther 334:467-76 (2010). WB ; Rat . PubMed: 20460386
      • Jamsa A  et al. The retinoic acid and brain-derived neurotrophic factor differentiated SH-SY5Y cell line as a model for Alzheimer's disease-like tau phosphorylation. Biochem Biophys Res Commun 319:993-1000 (2004). Human . PubMed: 15184080

      レビューと Q&A

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      1-4 of 4 Abreviews or Q&A

      Western blot abreview for Anti-GSK3 (alpha + beta) (phospho Y216 + Y279) antibody

      Poor
      Abreviews
      Abreviews
      abreview image
      Application
      Western blot
      Sample
      Human Cell lysate - whole cell (ARPE-19)
      Gel Running Conditions
      Reduced Denaturing
      Loading amount
      15 µg
      Treatment
      125 ug/ml Cigarette Smoke Extract
      Specification
      ARPE-19
      Blocking step
      BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
      Read More

      DR. Lei Wang

      Verified customer

      投稿 Jul 25 2017

      Western blot abreview for Anti-GSK3 (alpha + beta) (phospho Y216 + Y279) antibody

      Excellent
      Abreviews
      Abreviews
      Application
      Western blot
      Sample
      Rat Tissue lysate - other (brain capillary membranes)
      Loading amount
      5 µg
      Specification
      brain capillary membranes
      Treatment
      Roche Diagnostics Complete Protease Inhibitor
      Gel Running Conditions
      Reduced Denaturing (Invitrogen NuPage Bis-Tris 4-12%, 1 mm x 15 wells)
      Blocking step
      Pierce Protein-free T20 (TBS) Blocking Buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 23°C
      Read More

      Abcam user community

      Verified customer

      投稿 Mar 02 2012

      Western blot abreview for Anti-GSK3 (alpha + beta) (phospho Y216 + Y279) antibody

      Good
      Abreviews
      Abreviews
      abreview image
      Application
      Western blot
      Sample
      Human Cell lysate - whole cell (huh-7)
      Loading amount
      20 µg
      Specification
      huh-7
      Gel Running Conditions
      Reduced Denaturing
      Blocking step
      Milk as blocking agent for 20 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
      Read More

      Abcam user community

      Verified customer

      投稿 Sep 29 2009

      Western blot abreview for Anti-GSK3 (alpha + beta) (phospho Y216 + Y279) antibody

      Excellent
      Abreviews
      Abreviews
      abreview image
      Application
      Western blot
      Sample
      Human Cell lysate - whole cell (HEK293T)
      Loading amount
      40 µg
      Specification
      HEK293T
      Treatment
      X gene transfection for 24hrs
      Gel Running Conditions
      Non-reduced Denaturing (10%)
      Blocking step
      Milk as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
      Read More

      Chien Hsin Lee

      Verified customer

      投稿 Sep 09 2009

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
      For licensing inquiries, please contact partnerships@abcam.com

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