Anti-GSDMD 抗体 - C-terminal (ab228824)
Key features and details
- Rabbit polyclonal to GSDMD - C-terminal
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-GSDMD antibody - C-terminal
GSDMD 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to GSDMD - C-terminal -
由来種
Rabbit -
アプリケーション
適用あり: WB, IPmore details -
種交差性
交差種: Human -
免疫原
Synthetic peptide within Human GSDMD aa 434-484 (C terminal). The exact sequence is proprietary.
Database link: P57764 -
ポジティブ・コントロール
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
バッファー
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA -
Concentration information loading...
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精製度
Immunogen affinity purified -
特記事項(精製)
ab228824 was affinity purified using an epitope specific to GSDMD immobilized on solid support. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab228824の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/1000. Predicted molecular weight: 52 kDa.
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IP |
Use at an assay dependent concentration.
Use at 20 µl/mg lysate. |
特記事項 |
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WB
1/1000. Predicted molecular weight: 52 kDa. |
IP
Use at an assay dependent concentration. Use at 20 µl/mg lysate. |
ターゲット情報
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配列類似性
Belongs to the gasdermin family. - Information by UniProt
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参照データベース
- Entrez Gene: 79792 Human
- Omim: 617042 Human
- SwissProt: P57764 Human
- Unigene: 118983 Human
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別名
- 1810036L03Rik antibody
- DF 5L antibody
- DF5L antibody
see all
画像
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GSDMD was immunoprecipitated from Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab228824 at 20 µl per reaction. Western blot was performed from the immunoprecipitate using ab228824 at 1/100 dilution.
Lane 1: ab228824 IP in Jurkat whole cell lysate.
Lane 2: Control IgG IP in Jurkat whole cell lysate.Detection:Chemiluminescence with exposure time of 30 seconds.
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All lanes : Anti-GSDMD antibody - C-terminal (ab228824) at 1/1000 dilution
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 52 kDa
Exposure time: 3 minutesLysates were prepared using NETN lysis buffer.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab228824 は 1 報の論文で使用されています。
- Liu Z et al. NLRP3 inflammasome of renal tubular epithelial cells induces kidney injury in acute hemolytic transfusion reactions. Clin Transl Med 11:e373 (2021). PubMed: 33783986