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Anti-GSDMC2 + GSDMC3 抗体 [EPR20890-48] - BSA and Azide free (ab269859)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GSDMC2+GSDMC3 antibody [EPR20890-48] - BSA and Azide free (ab269859)
  • Western blot - Anti-GSDMC2+GSDMC3 antibody [EPR20890-48] - BSA and Azide free (ab269859)
  • Immunohistochemistry (Frozen sections) - Anti-GSDMC2+GSDMC3 antibody [EPR20890-48] - BSA and Azide free (ab269859)
  • Western blot - Anti-GSDMC2+GSDMC3 antibody [EPR20890-48] - BSA and Azide free (ab269859)
  • Immunohistochemistry (Frozen sections) - Anti-GSDMC2+GSDMC3 antibody [EPR20890-48] - BSA and Azide free (ab269859)
  • Western blot - Anti-GSDMC2+GSDMC3 antibody [EPR20890-48] - BSA and Azide free (ab269859)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GSDMC2+GSDMC3 antibody [EPR20890-48] - BSA and Azide free (ab269859)
  • Anti-GSDMC2 + GSDMC3 antibody [EPR20890-48] - BSA and Azide free (ab269859)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR20890-48] to GSDMC2 + GSDMC3 - BSA and Azide free
  • Suitable for: IHC-Fr, WB, IHC-P
  • Knockout validated
  • Reacts with: Mouse

製品の概要

  • 製品名

    Anti-GSDMC2 + GSDMC3 antibody [EPR20890-48] - BSA and Azide free
    GSDMC2+GSDMC3 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR20890-48] to GSDMC2 + GSDMC3 - BSA and Azide free

  • 由来種

    Rabbit

  • Tested Applications & Species

    Application Species
    IHC-Fr
    Mouse
    IHC-P
    Mouse
    WB
    Mouse
    See all applications and species data

  • 免疫原

    This product was produced with the following immunogens:
    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • WB: Mouse stomach, small intestine, colon and caecum tissue lysates; HEK-293T transfected with mGSDMC2 expression vector, whole cell lysate; HEK-293T transfected with mGSDMC3 expression vector, whole cell lysate. IHC-P: Mouse stomach tissue. IHC-Fr: Mouse stomach and colon tissue.

  • 特記事項

    ab269859 is the carrier-free version of ab229896. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab269859 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Store at +4°C.

  • バッファー

    Constituent: 100% PBS

  • キャリア・フリー

    はい
  • Concentration information loading...

  • 精製度

    Protein A purified

  • ポリ/モノ

    モノクローナル

  • クローン名

    EPR20890-48

  • アイソタイプ

    IgG

アプリケーション

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab269859 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

アプリケーション Species
IHC-Fr
Mouse
IHC-P
Mouse
WB
Mouse
アプリケーション Abreviews 特記事項
IHC-Fr
Use at an assay dependent concentration.

Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).
WB
Use at an assay dependent concentration. Detects a band of approximately 54, 56 kDa (predicted molecular weight: 54 kDa).
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
特記事項
IHC-Fr
Use at an assay dependent concentration.

Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).
WB
Use at an assay dependent concentration. Detects a band of approximately 54, 56 kDa (predicted molecular weight: 54 kDa).
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
  • 追加情報
    Is unsuitable for Flow Cyt or IP.

    • ターゲット情報

    画像

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GSDMC2+GSDMC3 antibody [EPR20890-48] - BSA and Azide free (ab269859)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GSDMC2+GSDMC3 antibody [EPR20890-48] - BSA and Azide free (ab269859)

      This data was developed using ab229896, the same antibody clone in a different buffer formulation.

      Immunohistochemical analysis of paraffin-embedded mouse stomach tissue labeling GSDMC2+GSDMC3 with abab229896 at 1/2000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Cytoplamic staining on epithelial cells in mouse stomach (PMID: 17350798). The section was incubated with ab229896 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

      Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

      Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

    • Western blot - Anti-GSDMC2+GSDMC3 antibody [EPR20890-48] - BSA and Azide free (ab269859)
      Western blot - Anti-GSDMC2+GSDMC3 antibody [EPR20890-48] - BSA and Azide free (ab269859)
      All lanes : Anti-GSDMC2 + GSDMC3 antibody [EPR20890-48] (ab229896) at 1/1000 dilution

      Lane 1 : Wild-type mouse esophagus tissue lysate
      Lane 2 : mGSDMC1-4 knockout mouse esophagus tissue lysate
      Lane 3 : Wild-type mouse stomach tissue lysate
      Lane 4 : mGSDMC1-4 knockout mouse stomach tissue lysate
      Lane 5 : Wild-type mouse small intestine tissue lysate
      Lane 6 : mGSDMC1-4 knockout mouse small intestine tissue lysate
      Lane 7 : Wild-type mouse colon tissue lysate
      Lane 8 : mGSDMC1-4 knockout mouse colon tissue lysate
      Lane 9 : Wild-type mouse caecum tissue lysate
      Lane 10 : mGSDMC1-4 knockout mouse caecum tissue lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100000 dilution

      Predicted band size: 54 kDa
      Observed band size: 56 kDa
      why is the actual band size different from the predicted?



      This data was developed using ab229896, the same antibody clone in a different buffer formulation. 

      Blocking and diluting buffer and concentration: 5% NFDM/TBST.

      The image was kindly provided by Dr. Jiahuai Han (Xiamen University, China).

      Exposure time: 60 seconds.

    • Immunohistochemistry (Frozen sections) - Anti-GSDMC2+GSDMC3 antibody [EPR20890-48] - BSA and Azide free (ab269859)
      Immunohistochemistry (Frozen sections) - Anti-GSDMC2+GSDMC3 antibody [EPR20890-48] - BSA and Azide free (ab269859)

      This data was developed using ab229896, the same antibody clone in a different buffer formulation.

      Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen mouse colon tissue labeling GSDMC2+GSDMC3 with ab229896 at 1/200 dilution followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution (Green). Positive staining on epithelial cells in mouse colon is observed. The nuclear counterstain was DAPI (Blue).

      Secondary antibody control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)at 1/1000 dilution.

      Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

    • Western blot - Anti-GSDMC2+GSDMC3 antibody [EPR20890-48] - BSA and Azide free (ab269859)
      Western blot - Anti-GSDMC2+GSDMC3 antibody [EPR20890-48] - BSA and Azide free (ab269859)
      All lanes : Anti-GSDMC2 + GSDMC3 antibody [EPR20890-48] (ab229896) at 1/1000 dilution

      Lane 1 : Mouse stomach tissue lysate
      Lane 2 : Mouse spleen tissue lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100000 dilution

      Predicted band size: 54 kDa
      Observed band size: 54 kDa



      This data was developed using ab229896, the same antibody clone in a different buffer formulation. 

      Blocking and diluting buffer and concentration: 5% NFDM/TBST.

      Negative control: spleen (PMID: 17350798).

      Exposure time: 54 seconds.

    • Immunohistochemistry (Frozen sections) - Anti-GSDMC2+GSDMC3 antibody [EPR20890-48] - BSA and Azide free (ab269859)
      Immunohistochemistry (Frozen sections) - Anti-GSDMC2+GSDMC3 antibody [EPR20890-48] - BSA and Azide free (ab269859)

      This data was developed using ab229896, the same antibody clone in a different buffer formulation.

      Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen mouse stomach tissue labeling GSDMC2+GSDMC3 with ab229896 at 1/200 dilution followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution (Green). Positive staining on epithelial cells in mouse stomach is observed. The nuclear counterstain was DAPI (Blue).

      Secondary antibody control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)at 1/1000 dilution.

      Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

    • Western blot - Anti-GSDMC2+GSDMC3 antibody [EPR20890-48] - BSA and Azide free (ab269859)
      Western blot - Anti-GSDMC2+GSDMC3 antibody [EPR20890-48] - BSA and Azide free (ab269859)
      All lanes : Anti-GSDMC2 + GSDMC3 antibody [EPR20890-48] (ab229896) at 1/1000 dilution

      Lane 1 : HEK-293T (human embryonic kidney) transfected with an empty vector (vector control), whole cell lysate
      Lane 2 : HEK-293T transfected with mGSDMC1 expression vector, whole cell lysate
      Lane 3 : HEK-293T transfected with mGSDMC2 expression vector, whole cell lysate
      Lane 4 : HEK-293T transfected with mGSDMC3 expression vector, whole cell lysate
      Lane 5 : HEK-293T transfected with mGSDMC4 expression vector, whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100000 dilution

      Predicted band size: 54 kDa
      Observed band size: 56 kDa why is the actual band size different from the predicted?



      This data was developed using ab229896, the same antibody clone in a different buffer formulation. 

      Blocking and diluting buffer and concentration: 5% NFDM/TBST.

      The image was kindly provided by Dr. Jiahuai Han (Xiamen University, China).

      Exposure time: 60 seconds.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GSDMC2+GSDMC3 antibody [EPR20890-48] - BSA and Azide free (ab269859)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GSDMC2+GSDMC3 antibody [EPR20890-48] - BSA and Azide free (ab269859)

      This data was developed using ab229896, the same antibody clone in a different buffer formulation.

      Immunohistochemical analysis of paraffin-embedded mouse spleen tissue labeling GSDMC2+GSDMC3 with abab229896 at 1/2000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Negative control: No staining on mouse spleen (PMID: 17350798). The section was incubated with ab229896 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

      Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

      Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

    • Anti-GSDMC2 + GSDMC3 antibody [EPR20890-48] - BSA and Azide free (ab269859)
      Anti-GSDMC2 + GSDMC3 antibody [EPR20890-48] - BSA and Azide free (ab269859)

    プロトコール

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    参考文献 (0)

    ab269859 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab269859 は論文での使用が確認できていません。

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