遺伝子ノックアウト細胞株 検証済リコンビナントRabMAb

Anti-GSDMC 抗体 [EPR20887-50] - BSA and Azide free (ab269858)

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Western blot - Anti-GSDMC antibody [EPR20887-50] - BSA and Azide free (ab269858)
  • Western blot - Anti-GSDMC antibody [EPR20887-50] - BSA and Azide free (ab269858)
  • Western blot - Anti-GSDMC antibody [EPR20887-50] - BSA and Azide free (ab269858)
  • Anti-GSDMC antibody [EPR20887-50] - BSA and Azide free (ab269858)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR20887-50] to GSDMC - BSA and Azide free
  • Suitable for: WB
  • Knockout validated
  • Reacts with: Mouse, Human

製品の概要

  • 製品名

    Anti-GSDMC antibody [EPR20887-50] - BSA and Azide free
    GSDMC 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR20887-50] to GSDMC - BSA and Azide free

  • 由来種

    Rabbit

  • Tested Applications & Species

    Application Species
    WB
    Mouse
    See all applications and species data

  • 免疫原

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • WB: Mouse stomach and esophagus tissue lysates; HEK-293T transfected with mGSDMC1 expression vector, whole cell lysate.

  • 特記事項

    The gene for mouse GSDMC is also known as GSDMC1

    ab269858 is the carrier-free version of ab225635. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab269858 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Store at +4°C.

  • バッファー

    Constituent: 100% PBS

  • キャリア・フリー

    はい
  • Concentration information loading...

  • 精製度

    Protein A purified

  • ポリ/モノ

    モノクローナル

  • クローン名

    EPR20887-50

  • アイソタイプ

    IgG

  • 研究分野

関連製品

アプリケーション

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab269858 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

アプリケーション Species
WB
Mouse
アプリケーション Abreviews 特記事項
WB
Use at an assay dependent concentration. Detects a band of approximately 52, 54 kDa (predicted molecular weight: 53 kDa).
特記事項
WB
Use at an assay dependent concentration. Detects a band of approximately 52, 54 kDa (predicted molecular weight: 53 kDa).
  • 追加情報
    Is unsuitable for Flow Cyt,ICC,IHC-Fr,IHC-P or IP.

    • ターゲット情報

    画像

    • Western blot - Anti-GSDMC antibody [EPR20887-50] - BSA and Azide free (ab269858)
      Western blot - Anti-GSDMC antibody [EPR20887-50] - BSA and Azide free (ab269858)
      All lanes : Anti-GSDMC antibody [EPR20887-50] (ab225635) at 1/1000 dilution

      Lane 1 : Mouse stomach tissue lysate
      Lane 2 : Mouse esophagus tissue lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100000 dilution

      Predicted band size: 53 kDa
      Observed band size: 52 kDa why is the actual band size different from the predicted?



      This data was developed using ab225635, the same antibody clone in a different buffer formulation. 

      Blocking and diluting buffer and concentration: 5% NFDM/TBST.

      Exposure times: Lane 1: 15 secondsLane 2: 3.25 seconds.

    • Western blot - Anti-GSDMC antibody [EPR20887-50] - BSA and Azide free (ab269858)
      Western blot - Anti-GSDMC antibody [EPR20887-50] - BSA and Azide free (ab269858)
      All lanes : Anti-GSDMC antibody [EPR20887-50] (ab225635) at 1/1000 dilution

      Lane 1 : Wild-type Mouse esophagus tissue lysate
      Lane 2 : mGSDMC1-4 knockout mouse esophagus tissue lysate
      Lane 3 : Wild-type Mouse stomach tissue lysate
      Lane 4 : mGSDMC1-4 knockout mouse stomach tissue lysate
      Lane 5 : Wild-type Mouse small intestine tissue lysate
      Lane 6 : mGSDMC1-4 knockout mouse small intestine tissue lysate
      Lane 7 : Wild-type Mouse colon tissue lysate
      Lane 8 : mGSDMC1-4 knockout mouse colon tissue lysate
      Lane 9 : Wild-type Mouse caecum tissue lysate
      Lane 10 : mGSDMC1-4 knockout mouse caecum tissue lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100000 dilution

      Predicted band size: 53 kDa
      Observed band size: 54 kDa why is the actual band size different from the predicted?



      This data was developed using ab225635, the same antibody clone in a different buffer formulation. 

      Blocking and diluting buffer and concentration: 5% NFDM/TBST.

      The image was kindly provided by Dr. Jiahuai Han (Xiamen University, China).

      Exposure time: 60 seconds.

    • Western blot - Anti-GSDMC antibody [EPR20887-50] - BSA and Azide free (ab269858)
      Western blot - Anti-GSDMC antibody [EPR20887-50] - BSA and Azide free (ab269858)
      All lanes : Anti-GSDMC antibody [EPR20887-50] (ab225635) at 1/1000 dilution

      Lane 1 : HEK-293T (human embryonic kidney) transfected with an empty vector (vector control), whole cell lysate
      Lane 2 : HEK-293T transfected with mGSDMC1 expression vector, whole cell lysate
      Lane 3 : HEK-293T transfected with mGSDMC2 expression vector, whole cell lysate
      Lane 4 : HEK-293T transfected with mGSDMC3 expression vector, whole cell lysate
      Lane 5 : HEK-293T transfected with mGSDMC4 expression vector, whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100000 dilution

      Predicted band size: 53 kDa
      Observed band size: 54 kDa why is the actual band size different from the predicted?



      This data was developed using ab225635, the same antibody clone in a different buffer formulation. 

      Blocking and diluting buffer and concentration: 5% NFDM/TBST.

      The image was kindly provided by Dr. Jiahuai Han (Xiamen University, China).

      Exposure time: 60 seconds.

    • Anti-GSDMC antibody [EPR20887-50] - BSA and Azide free (ab269858)
      Anti-GSDMC antibody [EPR20887-50] - BSA and Azide free (ab269858)

    プロトコール

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    参考文献 (0)

    ab269858 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab269858 は論文での使用が確認できていません。

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